Germ cell protocols. Part 2, Molecular embryo analysis, live imaging, transgenesis, and cloning / edited by Heide Schatten.

Contributor(s): Schatten, Heide
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): volume 254, pt. 2.Publisher: Totowa, N.J. : Humana Press, ©2004Description: 1 online resource (1 volume) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 1592597416; 9781592597413; 9781588292575; 1588292576Other title: Molecular embryo analysis, live imaging, transgenesis, and cloningSubject(s): Veterinary embryology -- Research -- Technique | Germ cells -- Research -- Technique | Cytological Techniques | Germ Cells | Genetic Techniques | MEDICAL -- Veterinary Medicine -- Food Animal | kiemcellen | germ cells | embryos | transgenen | transgenics | embryokloneren | embryo cloning | genetische analyse | genetic analysis | laboratoriummethoden | laboratory methods | technieken | techniques | celbiologie | cellular biology | moleculaire biologie | molecular biology | protocollen | protocols | Reproduction and Fertility | Cellular Biology | Voortplanting en vruchtbaarheid | CelbiologieGenre/Form: Electronic books. Additional physical formats: Print version:: Germ cell protocols. Volume 2, Molecular embryo analysis, live imaging, transgenesis, and cloning.DDC classification: 636.089/264 LOC classification: SF767.5Online resources: Click here to access online
Contents:
v. 1. Sperm and oocyte analysis -- v. 2. Molecular embryo analysis, live imaging, transgenesis, and cloning.
Activity of the germline-specific oct4-GFP transgene in normal and clone mouse embryos / M. Boiani, J. Kehler and H.R. Scholer -- Analysis of germline chromatin silencing by double-stranded RNA-mediated interference (RNAi) in caenorhabditis elegans / M.A. Jedrusik and E. Schulze -- cDNA subtraction and cloning in the field of trophoblast/placental development / M. Hemberger -- PCR-based cloning and differential screening of rnas from xenopus primordial germ cells: Cloning uniquely expressed rnas from rare cells / T. Venkataraman, E. Dancausse and M.L. King -- Differentiating embryonic stem cells into embryoid bodies / G. Hopfl, M. Gassmann and I. Desbaillets -- The derivation of murine embryonic stem and embryonic germ cells by selective ablation / E.J. Gallagher and J. McWhir -- Using multiphoton excitation to explore the murky depths of developing embryos / J.M. Squirrell and J.G. White -- Dual-channel confocal ratioing of calcium dynamics in living eggs and oocytes / S.A. Stricker -- A method for producing cloned pigs by using somatic cells as donors / L. Lai and R.S. Prather -- Pronuclear microinjection of mouse zygotes / T. Rulicke -- Parthenogenesis and nuclear transfer in rabbit oocytes / Y. Tsunoda and Y. Kato -- Nuclear transfer in cattle / G. Brem and M.A. Nowshari -- In vitro fertilization and embryo transfer in felids / C.E. Pope -- Production of chimeric chickens / M. Naito and T. Kuwana -- Production of haploid and diploid androgenetic zebrafish / B.P. Brandhorst and G.E. Corley-Smith -- Construction and detection of fluorescent, germline transgenic zebrafish / E. Linney and A.J. Udvadia -- Production of zebrafish germline chimeras from cultured cells / L. Fan [and others] -- Studying nuclear reprogramming with cell hybrids / S. Pells and J. McWhir -- Cytoplasmic transfer in mammalian eggs and embryos / H.E. Malter -- Conventional technologies for cryopreservation of human oocytes and embryos / M.J. Tucker, S. Shipley and J. Liebermann -- Vitrifying and warming of human oocytes, embryos, and blastocysts: Vitrification procedures as an alternative to conventional cryopreservation / J. Liebermann and M.J. Tucker -- Microfluidic removal of cumulus cells from mammalian zygotes / H.C. Zeringue and D.J. Beebe -- Mammalian embryo culture in a microfluidic device / E.M. Walters [and others] -- Application of alginate gels to the study of mammalian development / R.L. Gardner -- Using tunel in combination with an active caspase-3 immunoassay to identify cells undergoing apoptosis in preimplantation mammalian embryos / S.F. Mullen and J.K. Critser.
Summary: Germ cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a series of powerful techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Chosen for their proven success in advancing our understanding of germ cells, these methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, the imaging of cell structures, and the cryopreservation of germ cells. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. There are also methods for PCR-based cloning, differentiating embryonic stem cells into embryonic bodies, live imaging of mammalian embryos using multiphoton microscopy, and the cloning of pig, cattle, rabbit, and mouse oocytes. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. An accompanying earlier volume, Sperm and Oocyte Analysis, focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods for high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.
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Includes bibliographical references and indexes.

v. 1. Sperm and oocyte analysis -- v. 2. Molecular embryo analysis, live imaging, transgenesis, and cloning.

Activity of the germline-specific oct4-GFP transgene in normal and clone mouse embryos / M. Boiani, J. Kehler and H.R. Scholer -- Analysis of germline chromatin silencing by double-stranded RNA-mediated interference (RNAi) in caenorhabditis elegans / M.A. Jedrusik and E. Schulze -- cDNA subtraction and cloning in the field of trophoblast/placental development / M. Hemberger -- PCR-based cloning and differential screening of rnas from xenopus primordial germ cells: Cloning uniquely expressed rnas from rare cells / T. Venkataraman, E. Dancausse and M.L. King -- Differentiating embryonic stem cells into embryoid bodies / G. Hopfl, M. Gassmann and I. Desbaillets -- The derivation of murine embryonic stem and embryonic germ cells by selective ablation / E.J. Gallagher and J. McWhir -- Using multiphoton excitation to explore the murky depths of developing embryos / J.M. Squirrell and J.G. White -- Dual-channel confocal ratioing of calcium dynamics in living eggs and oocytes / S.A. Stricker -- A method for producing cloned pigs by using somatic cells as donors / L. Lai and R.S. Prather -- Pronuclear microinjection of mouse zygotes / T. Rulicke -- Parthenogenesis and nuclear transfer in rabbit oocytes / Y. Tsunoda and Y. Kato -- Nuclear transfer in cattle / G. Brem and M.A. Nowshari -- In vitro fertilization and embryo transfer in felids / C.E. Pope -- Production of chimeric chickens / M. Naito and T. Kuwana -- Production of haploid and diploid androgenetic zebrafish / B.P. Brandhorst and G.E. Corley-Smith -- Construction and detection of fluorescent, germline transgenic zebrafish / E. Linney and A.J. Udvadia -- Production of zebrafish germline chimeras from cultured cells / L. Fan [and others] -- Studying nuclear reprogramming with cell hybrids / S. Pells and J. McWhir -- Cytoplasmic transfer in mammalian eggs and embryos / H.E. Malter -- Conventional technologies for cryopreservation of human oocytes and embryos / M.J. Tucker, S. Shipley and J. Liebermann -- Vitrifying and warming of human oocytes, embryos, and blastocysts: Vitrification procedures as an alternative to conventional cryopreservation / J. Liebermann and M.J. Tucker -- Microfluidic removal of cumulus cells from mammalian zygotes / H.C. Zeringue and D.J. Beebe -- Mammalian embryo culture in a microfluidic device / E.M. Walters [and others] -- Application of alginate gels to the study of mammalian development / R.L. Gardner -- Using tunel in combination with an active caspase-3 immunoassay to identify cells undergoing apoptosis in preimplantation mammalian embryos / S.F. Mullen and J.K. Critser.

Print version record.

Germ cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a series of powerful techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Chosen for their proven success in advancing our understanding of germ cells, these methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, the imaging of cell structures, and the cryopreservation of germ cells. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. There are also methods for PCR-based cloning, differentiating embryonic stem cells into embryonic bodies, live imaging of mammalian embryos using multiphoton microscopy, and the cloning of pig, cattle, rabbit, and mouse oocytes. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. An accompanying earlier volume, Sperm and Oocyte Analysis, focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods for high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.

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