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Embryonic stem cell protocols. Volume 1, Isolation and characterization / edited by Kursad Turksen.

Contributor(s): Turksen, KursadMaterial type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; volume 329, pt. 1.Publication details: Totowa, N.J. : Humana Press, ©2006. Edition: 2nd edDescription: 1 online resource (1 volume) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1597450375; 9781597450379; 1588294986; 9781588294982; 1588297845; 9781588297846; 1280358505; 9781280358500; 9786610358502; 6610358508Subject(s): Stem cells -- Laboratory manuals | Embryos -- Laboratory manuals | Stem Cells | Embryo Research | Models, Animal | Cris (Indiens) -- Religion | MEDICAL -- Research | Embryos | Stem cells | Biology | Health & Biological Sciences | Cytology | embryonale stamcellen | embryonic stem cells | isolatie | isolation | karakterisering | characterization | verjonging | regeneration | biologische technieken | biological techniques | celbiologie | cellular biology | moleculaire biologie | molecular biology | geneeskunde | medicine | protocollen | protocols | Cellular Biology | Medicine (General) | Celbiologie | Geneeskunde (algemeen)Genre/Form: Electronic books. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Embryonic stem cell protocols. Volume 1, Isolation and characterization.DDC classification: 616/.02774 LOC classification: QH588.S83 | E46eb vol. 1NLM classification: W1 | QU 325Online resources: Click here to access online
Contents:
v. 1. Isolation and characterization -- v. 2. Differentiation models.
Isolation and differentiation of medaka embryonic stem cells / Y. Hong and M. Schartl -- Maintenance of chicken embryonic stem cells in vitro / H. Horiuchi, S. Furusawa and H. Matsuda -- Derivation and culture of mouse trophoblast stem cells in vitro / S. Tanaka -- Derivation, maintenance, and characterization of rat embryonic stem cells in vitro / M. Schulze [and others] -- Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells / S. Saito [and others] -- Generation and characterization of monkey embryonic stem cells / H. Suemori and N. Nakatsuji -- Derivation and propagation of embryonic stem cells in serum- and feeder-free culture / J. Nichols and Q.L. Ying -- Internal standards in differentiating embryonic stem cells in vitro / C.L. Murphy -- Matrix assembly, cell polarization, and cell survival: Analysis of peri-implantation development with cultured embryonic stem cells / S. Li and P.D. Yurchenco -- Phosphoinositides, inositol phosphates, and phospholipase c in embryonic stem cells / L.R. Quinlan -- Cripto signaling in differentiating embryonic stem cells / G. Minchiotti, S. Parisi and M.G. Persico -- The use of embryonic stem cells to study hedgehog signaling / S. Becker and L. Grabel -- Transfection and promoter analysis in embryonic stem cells / S. Chung and K.S. Kim -- Sage analysis to identify embryonic stem cell-predominant transcripts / K.R. Boheler and K.V. Tarasov -- Utilization of digital differential display to identify novel targets of oct3/4 / Y. Tokuzawa, M. Maruyama and S. Yamanaka -- Gene silencing using rna interference in embryonic stem cells / J.M. Velkey [and others] -- Efficient transfer of hsv-1 amplicon vectors into embryonic stem cells and their derivatives / D. Riethmacher, F. Lim and T. Schimmang -- Lentiviral vector-mediated gene transfer in embryonic stem cells / M. Oka [and others] -- Use of the cytomegalovirus promoter for transient and stable transgene expression in mouse embryonic stem cells / K.M. Barrow, F.M. Perez-Campo and C.M. Ward -- Use of simian immunodeficiency virus vectors for simian embryonic stem cells / T. Asano, H. Shibata and Y. Hanazono -- Generation of green fluorescent protein-expressing monkey embryonic stem cells / T. Takada [and others] -- DNA damage response and mutagenesis in mouse embryonic stem cells / Y. Hong, R.B. Cervantes and P.J. Stambrook -- Ultraviolet-induced apoptosis in embryonic stem cells in vitro / D. Xu, T.J. Wilson and P.J. Hertzog -- Use of differentiating embryonic stem cells in pharmacological studies / B. Wdziekonski [and others] -- Embryonic stem cells as a source of differentiated neural cells for pharmacological screens / P.J. Mee [and others] -- Use of murine embryonic stem cells in embryotoxicity assays: The embryonic stem cell test / A.E. Seiler [and others] -- Use of chemical mutagenesis in mouse embryonic stem cells / S. Becker, M.H. de Angelis and J. Beckers -- Nuclear reprogramming of somatic nucleus hybridized with embryonic stem cells by electrofusion / M. Tada and T. Tada -- Methylation in embryonic stem cells in vitro / K. Nishino [and others] -- Identification of genes involved in tumor-like properties of embryonic stem cells / K. Takahashi, T. Ichisaka and S. Yamanaka -- In vivo tumor formation from primate embryonic stem cells / T. Asano [and others] -- Directed differentiation and characterization of genetically modified embryonic stem cells for therapy / A.A. Lau [and others] -- Use of differentiating embryonic stem cells in the parkinsonian mouse model / F. Nishimura [and others].
Summary: Drawing on the dramatically increasing research on embryonic stem (ES) cell biology and differentiation, Kursad Turksen has completely updated and expanded his highly acclaimed first edition of Embryonic Stem Cells: Methods and Protocols into two volumes. Focusing on ES cells recently isolated from different nonhuman species, volume one of Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. A companion second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, the two volumes of Embryonic Stem Cells illuminate for both novices and experts not only our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications, but also provide detailed accounts of the tools required for successful work in the area.
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v. 1. Isolation and characterization -- v. 2. Differentiation models.

Isolation and differentiation of medaka embryonic stem cells / Y. Hong and M. Schartl -- Maintenance of chicken embryonic stem cells in vitro / H. Horiuchi, S. Furusawa and H. Matsuda -- Derivation and culture of mouse trophoblast stem cells in vitro / S. Tanaka -- Derivation, maintenance, and characterization of rat embryonic stem cells in vitro / M. Schulze [and others] -- Derivation, maintenance, and induction of the differentiation in vitro of equine embryonic stem cells / S. Saito [and others] -- Generation and characterization of monkey embryonic stem cells / H. Suemori and N. Nakatsuji -- Derivation and propagation of embryonic stem cells in serum- and feeder-free culture / J. Nichols and Q.L. Ying -- Internal standards in differentiating embryonic stem cells in vitro / C.L. Murphy -- Matrix assembly, cell polarization, and cell survival: Analysis of peri-implantation development with cultured embryonic stem cells / S. Li and P.D. Yurchenco -- Phosphoinositides, inositol phosphates, and phospholipase c in embryonic stem cells / L.R. Quinlan -- Cripto signaling in differentiating embryonic stem cells / G. Minchiotti, S. Parisi and M.G. Persico -- The use of embryonic stem cells to study hedgehog signaling / S. Becker and L. Grabel -- Transfection and promoter analysis in embryonic stem cells / S. Chung and K.S. Kim -- Sage analysis to identify embryonic stem cell-predominant transcripts / K.R. Boheler and K.V. Tarasov -- Utilization of digital differential display to identify novel targets of oct3/4 / Y. Tokuzawa, M. Maruyama and S. Yamanaka -- Gene silencing using rna interference in embryonic stem cells / J.M. Velkey [and others] -- Efficient transfer of hsv-1 amplicon vectors into embryonic stem cells and their derivatives / D. Riethmacher, F. Lim and T. Schimmang -- Lentiviral vector-mediated gene transfer in embryonic stem cells / M. Oka [and others] -- Use of the cytomegalovirus promoter for transient and stable transgene expression in mouse embryonic stem cells / K.M. Barrow, F.M. Perez-Campo and C.M. Ward -- Use of simian immunodeficiency virus vectors for simian embryonic stem cells / T. Asano, H. Shibata and Y. Hanazono -- Generation of green fluorescent protein-expressing monkey embryonic stem cells / T. Takada [and others] -- DNA damage response and mutagenesis in mouse embryonic stem cells / Y. Hong, R.B. Cervantes and P.J. Stambrook -- Ultraviolet-induced apoptosis in embryonic stem cells in vitro / D. Xu, T.J. Wilson and P.J. Hertzog -- Use of differentiating embryonic stem cells in pharmacological studies / B. Wdziekonski [and others] -- Embryonic stem cells as a source of differentiated neural cells for pharmacological screens / P.J. Mee [and others] -- Use of murine embryonic stem cells in embryotoxicity assays: The embryonic stem cell test / A.E. Seiler [and others] -- Use of chemical mutagenesis in mouse embryonic stem cells / S. Becker, M.H. de Angelis and J. Beckers -- Nuclear reprogramming of somatic nucleus hybridized with embryonic stem cells by electrofusion / M. Tada and T. Tada -- Methylation in embryonic stem cells in vitro / K. Nishino [and others] -- Identification of genes involved in tumor-like properties of embryonic stem cells / K. Takahashi, T. Ichisaka and S. Yamanaka -- In vivo tumor formation from primate embryonic stem cells / T. Asano [and others] -- Directed differentiation and characterization of genetically modified embryonic stem cells for therapy / A.A. Lau [and others] -- Use of differentiating embryonic stem cells in the parkinsonian mouse model / F. Nishimura [and others].

Print version record.

Includes bibliographical references and indexes.

Drawing on the dramatically increasing research on embryonic stem (ES) cell biology and differentiation, Kursad Turksen has completely updated and expanded his highly acclaimed first edition of Embryonic Stem Cells: Methods and Protocols into two volumes. Focusing on ES cells recently isolated from different nonhuman species, volume one of Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. A companion second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, the two volumes of Embryonic Stem Cells illuminate for both novices and experts not only our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications, but also provide detailed accounts of the tools required for successful work in the area.

English.

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