Ion channel localization methods and protocols / edited by Anatoli N. Lopatin and Colin G. Nichols.

Contributor(s): Lopatin, Anatoli N | Nichols, Colin G
Material type: TextTextSeries: Methods in pharmacology and toxicology: Publisher: Totowa, NJ : Humana Press, ©2001Description: 1 online resource (xv, 502 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 0896038335; 9780896038332; 1592591183; 9781592591183; 1280820934; 9781280820939; 9786610820931; 6610820937Subject(s): Ion channels -- Research -- Methodology | Clinical Laboratory Techniques | Membrane Glycoproteins | Membrane Transport Proteins | Investigative Techniques | Membrane Proteins | Carrier Proteins | Proteins | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Amino Acids, Peptides, and Proteins | Chemicals and Drugs | Cytological Techniques | Ion Channels | SCIENCE -- Life Sciences -- Cell Biology | Ion channels -- Research -- Methodology | Fluoreszenzmikroskopie | Grün fluoreszierendes Protein | Ionenkanal | Methode | PharmakologieGenre/Form: Electronic books. Additional physical formats: Print version:: Ion channel localization methods and protocols.; Online version:: Ion channel localization methods and protocols.DDC classification: 571.6/4 LOC classification: QH603.I54 | I54 2001Other classification: WC 5100 Online resources: Click here to access online
Contents:
""Ion Channel Localization""; ""Preface""; ""Contents""; ""Contributors""; ""COLOR PLATES""; ""Part I. PHARMACOLOGICAL LABELING OF ION CHANNELS AND RECEPTORS""; ""1 Fluorescent Calcium Antagonists: Tools for Imaging of L-Type Calcium Channels in Living Cells""; ""2 Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies""; ""3 GABA-A Receptors: Autoradiographic Localization of Different Ligand Sites and Identification of Subtypes of the Benzodiazepine Binding Site""; ""4 Localization of ATP P2X Receptors""
""5 Small Conductance Calcium-Activated Potassium Channels in Rat Brain: Autoradiographic Localization Using Two Specific Toxins, Apamin and Scyllatoxin""""6 Mapping N-Type Calcium Channel Distributions with omega-Conotoxins""; ""7 Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development""; ""8 High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels""; ""Part II. APPLICATIONS OF GREEN FLUORESCENT PROTEIN (GFP) TECHNOLOGY""; ""9 Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells""
""10 Confocal Imaging of GFP-Labeled Ion Channels""""11 Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes""; ""12 Applications of Green Fluorescent Protein (GFP) Technology: Watching Ion Channel Biogenesis in Living Cells Using GFP Fusion Constructs""; ""13 Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models""; ""14 Mutant GFP-Based FRET Analysis of K+ Channel Organization""; ""15 Delivering Ion Channels to Mammalian Cells by Membrane Fusion""
""Part III. FUNCTIONAL ASSAYS IN ION CHANNEL LOCALIZATION""""16 Detection of Neurons Expressing Calcium-Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake""; ""17 Crystallization Technique for Localizing Ion Channels in Living Cells""; ""18 Imaging of Localized Neuronal Calcium Influx""; ""19 Using Caged Compounds to Map Functional Neurotransmitter Receptors""; ""Part IV. ATOMIC FORCE MICROSCOPY""; ""20 Atomic Force Microscopy of Reconstituted Ion Channels""; ""21 Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy""
""22 Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy""""23 Dynamic AFM of Patch Clamped Membranes""; ""24 Localizing Ion Channels with Scanning Probe Microscopes: A Perspective""; ""Index""
Action note: digitized 2010 committed to preserveSummary: Although there are many useful books on virtually every aspect of ion channel structure and function, work on channel localization-determining the specific sites of ion channels and their receptors on cells-has long been neglected. In Ion Channel Localization: Methods and Protocols, active researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact cell sites of such channels and receptors. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies. Comprehensive and rich in practical detail, Ion Channel Localization: Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms.
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Includes bibliographical references and index.

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Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. MiAaHDL

Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL

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Although there are many useful books on virtually every aspect of ion channel structure and function, work on channel localization-determining the specific sites of ion channels and their receptors on cells-has long been neglected. In Ion Channel Localization: Methods and Protocols, active researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact cell sites of such channels and receptors. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies. Comprehensive and rich in practical detail, Ion Channel Localization: Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms.

""Ion Channel Localization""; ""Preface""; ""Contents""; ""Contributors""; ""COLOR PLATES""; ""Part I. PHARMACOLOGICAL LABELING OF ION CHANNELS AND RECEPTORS""; ""1 Fluorescent Calcium Antagonists: Tools for Imaging of L-Type Calcium Channels in Living Cells""; ""2 Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies""; ""3 GABA-A Receptors: Autoradiographic Localization of Different Ligand Sites and Identification of Subtypes of the Benzodiazepine Binding Site""; ""4 Localization of ATP P2X Receptors""

""5 Small Conductance Calcium-Activated Potassium Channels in Rat Brain: Autoradiographic Localization Using Two Specific Toxins, Apamin and Scyllatoxin""""6 Mapping N-Type Calcium Channel Distributions with omega-Conotoxins""; ""7 Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development""; ""8 High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels""; ""Part II. APPLICATIONS OF GREEN FLUORESCENT PROTEIN (GFP) TECHNOLOGY""; ""9 Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells""

""10 Confocal Imaging of GFP-Labeled Ion Channels""""11 Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes""; ""12 Applications of Green Fluorescent Protein (GFP) Technology: Watching Ion Channel Biogenesis in Living Cells Using GFP Fusion Constructs""; ""13 Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models""; ""14 Mutant GFP-Based FRET Analysis of K+ Channel Organization""; ""15 Delivering Ion Channels to Mammalian Cells by Membrane Fusion""

""Part III. FUNCTIONAL ASSAYS IN ION CHANNEL LOCALIZATION""""16 Detection of Neurons Expressing Calcium-Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake""; ""17 Crystallization Technique for Localizing Ion Channels in Living Cells""; ""18 Imaging of Localized Neuronal Calcium Influx""; ""19 Using Caged Compounds to Map Functional Neurotransmitter Receptors""; ""Part IV. ATOMIC FORCE MICROSCOPY""; ""20 Atomic Force Microscopy of Reconstituted Ion Channels""; ""21 Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy""

""22 Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy""""23 Dynamic AFM of Patch Clamped Membranes""; ""24 Localizing Ion Channels with Scanning Probe Microscopes: A Perspective""; ""Index""

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