Glycoprotein methods and protocols : the mucins / edited by Anthony P. Corfield.

Contributor(s): Corfield, Anthony P
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 125.Publisher: Totowa, N.J. : Humana Press, ©2000Description: 1 online resource (xvii, 506 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592590483; 0896037207; 9780896037205; 1592590489; 1280820403; 9781280820403; 9786610820405; 6610820406Subject(s): Mucins -- Laboratory manuals | Mucoproteins | Glycoproteins | Proteins | Amino Acids, Peptides, and Proteins | Chemicals and Drugs | Mucins | SCIENCE -- Life Sciences -- Biochemistry | Mucines -- analyse | Glycoprotéines | Mucins | Wetenschappelijke technieken | Molekularbiologie | Methode | Mucine | Mucines | GlycoprotéinesGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Glycoprotein methods and protocols.DDC classification: 572/.68 LOC classification: QP552.M8 | G59 2000Online resources: Click here to access online
Contents:
Glycoprotein Methods and Protocols: The Mucins -- Preface -- Contents -- Contributors -- PART I PURIFICATION OF MUCIN -- 1 Isolation of Large Gel-Forming Mucins -- 2 Preparation of Membrane Mucin -- PART II DETECTION AND QUANTITATION OF MUCIN -- 3 Histologically Based Methods for Detection of Mucin -- 4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods -- 5 The Gastrointestinal Adherent Mucous Gel Barrier -- 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling
PART III SEPARATION, IDENTIFICATION, AND PHYSICAL CHARACTERIZATION OF MUCINS7 Separation and Identification of Mucins and Their Glycoforms -- 8 Heterogeneity and Size Distribution of Gel-Forming Mucins -- PART IV RHEOLOGY OF MUCIN -- 9 Rheology of Mucin -- PART V MUCIN PEPTIDE ANALYSIS -- 10 Amino Acid Analysis of Mucins -- 11 Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation -- 12 Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies -- 13 Mucin Domains to Explore Disulfide-Dependent Dimer Formation
PART VI CARBOHYDRATE STRUCTURAL ANALYSIS OF MUCINS14 Monosaccharide Composition of Mucins -- 15 O-Linked Oligosaccharide Chain Release and Fractionation -- 16 Structural Analysis of Mucin-Type O-Linked Oligosaccharides -- 17 Measurement of Sulfate in Mucins -- PART VII MUCIN BIOSYNTHESIS -- 18 Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study -- 19 Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin -- 20 Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis
21 Mucin Precursors: Identification and Analysis of Their Intracellular Processing22 Inhibition of Mucin Glycosylation -- 23 O-Linked Chain Glycosyltransferases -- PART VIII MUCIN GENE DETECTION -- 24 Mucin cDNA Cloning -- 25 Northern Blot Analysis of Large mRNAs -- 26 Southern Blot Analysis of Large DNA Fragments -- 27 In Situ Hybridization Techniques for Localizing Mucin mRNA -- 28 Detection of Mucin Gene Polymorphism -- PART IX PREPARATION OF ANTIMUCIN ANTIBODIES -- 29 Polyclonal and Monoclonal Techniques -- 30 Monoclonal Antibodies to Mucin VNTR Peptides
PART X ENZYMATIC DEGRADATION OF MUCINS31 Mucinase Activity -- 32 Proteinase Activity -- 33 Glycosidase Activity -- 34 Assays for Bacterial Mucin-Desulfating Sulfatases -- PART XI MUCIN-BACTERIAL INTERACTIONS -- 35 Mucin-Bacterial Binding Assays -- 36 Growth of Mucin Degrading Bacteria in Biofilms -- PART XII CELLULAR AND HUMORAL RESPONSES TO MUCINS -- 37 Generation of MUC1 Cytotoxic T-Cells in Mice and Epitope Mapping -- 38 Analysis of the Frequency of MHC-Unrestricted MUC1-Specific Cytotoxic T-Cells in Peripheral Blood by Limiting Dilution Assay
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: Anthony P. Corfield and a group of hands-on expermentalists bring together in Glycoprotein Methods and Protocols: The Mucins a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular biological and biochemical reagents. Also included are methods for the assessment of mucin peptide and carbohydrate, of mucin biosynthesis and degradation, and of mucin bacteriology and cell biology. The techniques take advantage of the latest improvements in sensitivity and specificity of detection, and in the preparation of new reagents for specific biochemical detection. Glycoprotein Methods and Protocols: The Mucins offers today's researchers a complete range of readily reproducible analytical techniques for studying mucins. Its powerful techniques constitute a launching pad for those entering the field for the first time and a state-of-the-art collection for those already active in mucin research.
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Includes bibliographical references and index.

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Anthony P. Corfield and a group of hands-on expermentalists bring together in Glycoprotein Methods and Protocols: The Mucins a state-of-the-art collection of reliable and tested methods for the study of all aspects of mucins. These powerful methods range from the preparation of mucins to their detection and quantitation with molecular biological and biochemical reagents. Also included are methods for the assessment of mucin peptide and carbohydrate, of mucin biosynthesis and degradation, and of mucin bacteriology and cell biology. The techniques take advantage of the latest improvements in sensitivity and specificity of detection, and in the preparation of new reagents for specific biochemical detection. Glycoprotein Methods and Protocols: The Mucins offers today's researchers a complete range of readily reproducible analytical techniques for studying mucins. Its powerful techniques constitute a launching pad for those entering the field for the first time and a state-of-the-art collection for those already active in mucin research.

Glycoprotein Methods and Protocols: The Mucins -- Preface -- Contents -- Contributors -- PART I PURIFICATION OF MUCIN -- 1 Isolation of Large Gel-Forming Mucins -- 2 Preparation of Membrane Mucin -- PART II DETECTION AND QUANTITATION OF MUCIN -- 3 Histologically Based Methods for Detection of Mucin -- 4 Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods -- 5 The Gastrointestinal Adherent Mucous Gel Barrier -- 6 Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling

PART III SEPARATION, IDENTIFICATION, AND PHYSICAL CHARACTERIZATION OF MUCINS7 Separation and Identification of Mucins and Their Glycoforms -- 8 Heterogeneity and Size Distribution of Gel-Forming Mucins -- PART IV RHEOLOGY OF MUCIN -- 9 Rheology of Mucin -- PART V MUCIN PEPTIDE ANALYSIS -- 10 Amino Acid Analysis of Mucins -- 11 Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation -- 12 Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies -- 13 Mucin Domains to Explore Disulfide-Dependent Dimer Formation

PART VI CARBOHYDRATE STRUCTURAL ANALYSIS OF MUCINS14 Monosaccharide Composition of Mucins -- 15 O-Linked Oligosaccharide Chain Release and Fractionation -- 16 Structural Analysis of Mucin-Type O-Linked Oligosaccharides -- 17 Measurement of Sulfate in Mucins -- PART VII MUCIN BIOSYNTHESIS -- 18 Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study -- 19 Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin -- 20 Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis

21 Mucin Precursors: Identification and Analysis of Their Intracellular Processing22 Inhibition of Mucin Glycosylation -- 23 O-Linked Chain Glycosyltransferases -- PART VIII MUCIN GENE DETECTION -- 24 Mucin cDNA Cloning -- 25 Northern Blot Analysis of Large mRNAs -- 26 Southern Blot Analysis of Large DNA Fragments -- 27 In Situ Hybridization Techniques for Localizing Mucin mRNA -- 28 Detection of Mucin Gene Polymorphism -- PART IX PREPARATION OF ANTIMUCIN ANTIBODIES -- 29 Polyclonal and Monoclonal Techniques -- 30 Monoclonal Antibodies to Mucin VNTR Peptides

PART X ENZYMATIC DEGRADATION OF MUCINS31 Mucinase Activity -- 32 Proteinase Activity -- 33 Glycosidase Activity -- 34 Assays for Bacterial Mucin-Desulfating Sulfatases -- PART XI MUCIN-BACTERIAL INTERACTIONS -- 35 Mucin-Bacterial Binding Assays -- 36 Growth of Mucin Degrading Bacteria in Biofilms -- PART XII CELLULAR AND HUMORAL RESPONSES TO MUCINS -- 37 Generation of MUC1 Cytotoxic T-Cells in Mice and Epitope Mapping -- 38 Analysis of the Frequency of MHC-Unrestricted MUC1-Specific Cytotoxic T-Cells in Peripheral Blood by Limiting Dilution Assay

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