Chemokine protocols / edited by Amanda E.I. Proudfoot, Timothy N.C. Wells, and Christine A. Power.
Contributor(s): Proudfoot, Amanda E. I | Wells, Timothy N. C | Power, ChristineMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): volume138.Publisher: Totowa, N.J. : Humana Press, ©2000Description: 1 online resource (xiii, 353 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592590582; 1592590586; 0896037223; 9780896037229Subject(s): Chemokines -- Analysis | Chemokines -- Receptors | Chemokines -- analysis | Chemokines -- physiology | Receptors, Chemokine -- analysis | Receptors, Chemokine -- physiology | MEDICAL -- Immunology | Wetenschappelijke technieken | chemokinen | chemokines | protocollen | protocols | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Fulltext. | Internet Resource. | Illustration. | Index not Present. | Laboratory Manual. Additional physical formats: Print version:: Chemokine protocols.DDC classification: 616.079 LOC classification: QR185.8.C45 | .C47 2000ebOnline resources: Click here to access online
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Includes bibliographical references and index.
Purification of chemokines from natural sources -- Cloning of novel chemokines using a signal sequence trap method -- Chemokine expression in insect cells -- Expression of chemokines in Escherichia coli -- Experession of chemokines in the periplasmic space of E. coli -- Synthesis of chemokines -- Identifcation of novel chemokines from expressed sequence of tag databases -- Purification of recombinant chemokines from E.coli -- Chemokine receptor cloning -- Generatin of stable cell lines expressing chemokine receptors -- Modified microchemotaxis assays -- Transwell chemotaxis -- Endothelial cell chemotaxis assays -- Radiolabeled chemokine binding assays -- Scintillation proximity binding assay -- Calcium mobilization -- Actin polymerization -- Reative oxygen release -- Histamine release -- Measurement of phosphoinositide 3-kinase activity -- Glycosaminogylcan binding assays -- CFU-A assay for measurement of the antiproliferative effects of chemokines on murine early hemopoietic progenitors -- Downmodulation and recycling of chemokine receptors -- Analysis of chemokine receptor endocytosis and recycling -- Chemokine inhibition of HIV infection -- The production of chemokine specific monoclonal antibodies: chemokine RANTES -- Monoclonal antibiotics to chemokine receptors -- Targeted expression of chemokines in vivo -- Chemokine knockout mice -- Chemokine receptor knockout mice -- Measurement of eosinophil accumulatin in vivo.
Print version record.
In the past decade research has established the biological importance of chemokines: they play a major role in leukocyte trafficking, in the recruitment of leukocytes to inflammatory sites, and are coreceptors along with CD4 for HIV cell entry. In Chemokine Protocols, expert investigators describe in detail important techniques usedchemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience. Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today's gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins.