Asthma : mechanisms and protocols / edited by K. Fan Chung and Ian Adcock.

Contributor(s): Chung, K. Fan, 1951- | Adcock, Ian
Material type: TextTextSeries: Methods in molecular medicine: 44.Publisher: Totowa, N.J. : Humana Press, ©2000Description: 1 online resource (xi, 346 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592590728; 1592590721Subject(s): Asthma -- Molecular aspects -- Laboratory manuals | Bronchial Diseases | Respiratory Hypersensitivity | Intercellular Signaling Peptides and Proteins | Investigative Techniques | Lung Diseases, Obstructive | Peptides | Proteins | Respiratory Tract Diseases | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Hypersensitivity, Immediate | Lung Diseases | Biological Factors | Amino Acids, Peptides, and Proteins | Disease | Chemicals and Drugs | Hypersensitivity | Immune System Diseases | Cytokines | Asthma | Clinical Laboratory Techniques | Asthme -- Aspect moléculaire -- Manuels de laboratoire | MEDICAL -- Pulmonary & Thoracic Medicine | HEALTH & FITNESS -- Diseases -- Respiratory | Asthma -- Molecular aspects | Wetenschappelijke techniekenGenre/Form: Collected Work. | Fulltext. | Internet Resource. | Laboratory Manuals. | Illustration. | Index not Present. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Asthma.DDC classification: 616.2/38/07 LOC classification: RC591 | .A826 2000Online resources: Click here to access online
Contents:
Asthma: Application of cell and molecular biology techniques to unravel causes and pathophysiological mechanisms / F. Chung and I. Adcock-Culture of normal human airway epithelial cells and measurement of mucin synthesis and secretion / R. Wu-Brush biopsy and culture of airway epithelial cells: Beta-adrenergic receptor system function / S.G. Kelsen, D. Ciccolella and K. Brennan-Isolation and characterization of human airway fibroblasts in culture / J. Chakir [and others]-Flow cytometric analysis of blood monocytes and alveolar macrophages / M.L. Taylor and B.S. Bochner-Expression of il-10 and gm-csf in blood monocytes and alveolar macrophages / M. John and S. Lim-Map kinase expression in eosinophils / P.M. de Souza [and others]-Human eosinophil isolation and the measurement of apoptosis / H. Kankaanranta [and others]-Adhesion of t-cells to airway smooth muscle cells / A.L. Lazaar and R.A. Panettieri, Jr.-Proliferation of circulating t-cells / M. Larche-Differential display analysis of inflammatory genes in airway epithelium / L.M. Schwiebert-Analysis of transcription factor activation: Nfkappab as a regulator of inflammatory genes in epithelial cells / R. Newton and I.M. Adcock-Transient transgenic approaches for investigating the role of gm-csf in pulmonary inflammation and immune diseases / Z. Xing, M.R. Stampfli and J. Gauldie-Quantitative analysis of rantes mrna in endobronchial biopsies using polymerase chain reaction / N. Berkman-Intracellular cytokine staining for analysis by flow cytometry / A.J. Frew, J. Madden and P. Bakakos-Localization of il-4 and il-5 mrna in bronchial biopsies / Y. Nakamura, P. Christodoulopoulos and Q. Hamid-Immunohistochemical analysis of adhesion molecules in airway biopsies / J.W.S and T.H. S-Site-directed mutagenesis in the investigation of beta-adrenoreceptor exosite / S.A. Green-Methods for determining beta(2)-adrenoreceptor genotype / C.D. J, P.W.A and P.H. I-Modulation of beta-adrenoreceptor expression in the airways / C.W.M. J-Chemical mutational analysis of glucocorticoid receptor / J.L.S and H.L. T-Histone acetylation and deacetylation / K. Ito, J.B.P and M.A. I-Genome-wide search for asthma genes / H.M.A and F.J.M.J.
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: As asthma increases in prevalence and severity around the world, the need for understanding its basic mechanisms and for developing better drugs and preventative measures becomes more urgent. In Asthma: Mechanisms and Protocols, K. Fan Chung and Ian Adcock bring together the first collection of laboratory methods for studying the molecular and cellular mechanisms of asthma. Leading experts describe highly effective methods for obtaining cells from asthmatic airways, analyzing gene and protein expression in these clinical samples, and using molecular and cellular tools to study cytokine expression and release in this disease. There are also cutting-edge techniques for studying asthma-related genes and genetic polymorphisms, and for understanding the effects of asthma treatment. User-friendly and state-of-the-art, Asthma: Mechanisms and Protocols provides all asthma researchers-whether novice or experienced, whether in experimental or clinical research-with a first-class collection of readily reproducible pharmacological, cellular, molecular, biochemical, and genetic methods for elucidating not only the mechanisms of the disease itself, but also of the drugs for asthma's treatment.
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Includes bibliographical references and index.

Asthma: Application of cell and molecular biology techniques to unravel causes and pathophysiological mechanisms / F. Chung and I. Adcock-Culture of normal human airway epithelial cells and measurement of mucin synthesis and secretion / R. Wu-Brush biopsy and culture of airway epithelial cells: Beta-adrenergic receptor system function / S.G. Kelsen, D. Ciccolella and K. Brennan-Isolation and characterization of human airway fibroblasts in culture / J. Chakir [and others]-Flow cytometric analysis of blood monocytes and alveolar macrophages / M.L. Taylor and B.S. Bochner-Expression of il-10 and gm-csf in blood monocytes and alveolar macrophages / M. John and S. Lim-Map kinase expression in eosinophils / P.M. de Souza [and others]-Human eosinophil isolation and the measurement of apoptosis / H. Kankaanranta [and others]-Adhesion of t-cells to airway smooth muscle cells / A.L. Lazaar and R.A. Panettieri, Jr.-Proliferation of circulating t-cells / M. Larche-Differential display analysis of inflammatory genes in airway epithelium / L.M. Schwiebert-Analysis of transcription factor activation: Nfkappab as a regulator of inflammatory genes in epithelial cells / R. Newton and I.M. Adcock-Transient transgenic approaches for investigating the role of gm-csf in pulmonary inflammation and immune diseases / Z. Xing, M.R. Stampfli and J. Gauldie-Quantitative analysis of rantes mrna in endobronchial biopsies using polymerase chain reaction / N. Berkman-Intracellular cytokine staining for analysis by flow cytometry / A.J. Frew, J. Madden and P. Bakakos-Localization of il-4 and il-5 mrna in bronchial biopsies / Y. Nakamura, P. Christodoulopoulos and Q. Hamid-Immunohistochemical analysis of adhesion molecules in airway biopsies / J.W.S and T.H. S-Site-directed mutagenesis in the investigation of beta-adrenoreceptor exosite / S.A. Green-Methods for determining beta(2)-adrenoreceptor genotype / C.D. J, P.W.A and P.H. I-Modulation of beta-adrenoreceptor expression in the airways / C.W.M. J-Chemical mutational analysis of glucocorticoid receptor / J.L.S and H.L. T-Histone acetylation and deacetylation / K. Ito, J.B.P and M.A. I-Genome-wide search for asthma genes / H.M.A and F.J.M.J.

Print version record.

As asthma increases in prevalence and severity around the world, the need for understanding its basic mechanisms and for developing better drugs and preventative measures becomes more urgent. In Asthma: Mechanisms and Protocols, K. Fan Chung and Ian Adcock bring together the first collection of laboratory methods for studying the molecular and cellular mechanisms of asthma. Leading experts describe highly effective methods for obtaining cells from asthmatic airways, analyzing gene and protein expression in these clinical samples, and using molecular and cellular tools to study cytokine expression and release in this disease. There are also cutting-edge techniques for studying asthma-related genes and genetic polymorphisms, and for understanding the effects of asthma treatment. User-friendly and state-of-the-art, Asthma: Mechanisms and Protocols provides all asthma researchers-whether novice or experienced, whether in experimental or clinical research-with a first-class collection of readily reproducible pharmacological, cellular, molecular, biochemical, and genetic methods for elucidating not only the mechanisms of the disease itself, but also of the drugs for asthma's treatment.

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