Protein sequencing protocols / edited by Bryan John Smith.

Contributor(s): Smith, Bryan John
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): volume 211.Publisher: Totowa, N.J. : Humana Press, ©2002Edition: 2nd edDescription: 1 online resource (xii, 493 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592593422; 1592593429; 0896039757; 9780896039759; 1280842253; 9781280842252; 9786610842254; 6610842256Subject(s): Nucleotide sequence -- Laboratory manuals | Molecular Sequence Data | Amino Acids, Peptides, and Proteins | Genetic Structures | Sequence Analysis | Chemicals and Drugs | Biochemical Phenomena | Genetic Phenomena | Genetic Techniques | Documentation | Information Services | Investigative Techniques | Phenomena and Processes | Chemical Phenomena | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Information Science | Molecular Structure | Sequence Analysis, Protein | Proteins | Base Sequence | Séquence nucléotidique -- Manuels de laboratoire | SCIENCE -- Life Sciences -- Genetics & Genomics | Nucleotide sequenceGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Protein sequencing protocols.DDC classification: 572.8/633 LOC classification: QP625.N89 | P76 2002Online resources: Click here to access online
Contents:
Preface -- Contributors -- 1 Strategies for Handling Polypeptides on a Micro-Scale -- Bryan John Smith and Paul Tempst -- 2 SDS Polyacrylamide Gel Electrophoresis -- for N-Terminal Protein Sequencing -- Bryan John Smith -- 3 Two-Dimensional Polyacrylamide Gel Electrophoresis -- for the Separation of Proteins for Chemical Characterization -- Michael J. Dunn -- 4 Electroblotting of Proteins from Polyacrylamide Gels -- for Chemical Characterization -- Michael J. Dunn -- 5 Enzymatic Cleavage of Proteins -- Bryan John Smith -- 6 Chemical Cleavage of Polypeptides -- Bryan John Smith -- 7 Enzymatic Preparation and Isolation of Glycopeptides -- David J. Harvey -- 8 Analytical and Micropreparative Capillary Electrophoresis of Peptides -- Alan J. Smith -- 9 High-Performance Liquid Chromatography On-Line Derivative -- Spectroscopy for the Characterization of Peptides -- with Aromatic Amino Acid Residues -- Christoph W. Turck -- 10 Hydrolysis of Samples for Amino Acid Analysis -- lan Davidson -- 11 Amino Acid Analysis: Precolumn Derivatization Methods -- G. Brent Irvine and lan Davidson -- 12 Post Column Amino Acid Analysis -- Alan J. Smith -- 13 Amino Acid Analysis Using Pre-Column Derivatization -- with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: -- Analysis of Hydrolyzed Proteins and Electroblotted Samples -- Steven A. Cohen -- 14 Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange -- Chromatography and IPAD Detection -- Petr Jandik, Jun Cheng, and Nebojsa Avdalovic -- 15 D-Amino Acid Analysis -- Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa -- 16 Validation of Amino Acid Analysis Methods -- Andrew J. Reason -- 17 Electrospray Mass Spectrometry of Peptides, Proteins -- and Glycoproteins -- Fiona M. Greer and Howard R. Morris -- 18 Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- Darryl J.C. Pappin -- 19 Nanoelectrospray Tandem Mass Spectrometry and Sequence -- Similarity Searching for Identification of Proteins -- from Organisms with Unknown Genomes -- Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, -- and Andrej Shevchenko -- 20 Direct Identification of Proteins in Ultracomplex Mixtures: -- Applications to Proteome Analysis -- David M. Schieltz and John R. Yates, 111 -- 21 Identification of PTH-Amino Acids by HPLC -- Gregory A. Grant and Mark W. Crankshaw -- 22 Protein Sequencer Maintenance and Troubleshooting -- Bryan Dunbar -- 23 N-Terminal Protein Sequencing for Special Applications -- Philip J. Jackson -- 24 Identification of Phosphorylation Sites by Edman Degradation -- Laurey Steinke and Richard G. Cook -- 25 Validation of Protein Sequencing in a Regulated Laboratory -- Philip J. Jackson and Neil Dodsworth -- 26 Automated C-Terminal Protein Sequence Analysis -- Using the Alkylated-Thiohydantoin Method -- David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham -- 27 Chemical Modifications of Proteins as an Aid to Sequence Analysis -- Alex F. Carne -- 28 Deblocking of N-Terminally Modified Proteins -- Hisashi Hirano and Roza M. Kamp -- 29 Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid -- Jacek Mozdzanowski -- 30 Identification of Sites of Glycosylation -- David J. Harvey -- 31 Analysis of Sites of Protein Phosphorylation -- Alastair Aitken and Michele Learmonth -- 32 Quantitation and Location of Disulfide Bonds in Proteins -- Alastair Aitken and Michele Learmonth -- 33 Getting the Most from Your Protein Sequence -- Richard R. Copley and Robert B. Russell -- Appendix 1: Letter Codes, Structures, Masses, and Derivatives -- of Amino Acids -- James P. Turner -- Appendix 2: Protein Consensus Sequence Motifs -- Alastair Aitken.
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Preface -- Contributors -- 1 Strategies for Handling Polypeptides on a Micro-Scale -- Bryan John Smith and Paul Tempst -- 2 SDS Polyacrylamide Gel Electrophoresis -- for N-Terminal Protein Sequencing -- Bryan John Smith -- 3 Two-Dimensional Polyacrylamide Gel Electrophoresis -- for the Separation of Proteins for Chemical Characterization -- Michael J. Dunn -- 4 Electroblotting of Proteins from Polyacrylamide Gels -- for Chemical Characterization -- Michael J. Dunn -- 5 Enzymatic Cleavage of Proteins -- Bryan John Smith -- 6 Chemical Cleavage of Polypeptides -- Bryan John Smith -- 7 Enzymatic Preparation and Isolation of Glycopeptides -- David J. Harvey -- 8 Analytical and Micropreparative Capillary Electrophoresis of Peptides -- Alan J. Smith -- 9 High-Performance Liquid Chromatography On-Line Derivative -- Spectroscopy for the Characterization of Peptides -- with Aromatic Amino Acid Residues -- Christoph W. Turck -- 10 Hydrolysis of Samples for Amino Acid Analysis -- lan Davidson -- 11 Amino Acid Analysis: Precolumn Derivatization Methods -- G. Brent Irvine and lan Davidson -- 12 Post Column Amino Acid Analysis -- Alan J. Smith -- 13 Amino Acid Analysis Using Pre-Column Derivatization -- with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate: -- Analysis of Hydrolyzed Proteins and Electroblotted Samples -- Steven A. Cohen -- 14 Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange -- Chromatography and IPAD Detection -- Petr Jandik, Jun Cheng, and Nebojsa Avdalovic -- 15 D-Amino Acid Analysis -- Andrea Scaloni, Maurizio Simmaco, and Francesco Bossa -- 16 Validation of Amino Acid Analysis Methods -- Andrew J. Reason -- 17 Electrospray Mass Spectrometry of Peptides, Proteins -- and Glycoproteins -- Fiona M. Greer and Howard R. Morris -- 18 Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- Darryl J.C. Pappin -- 19 Nanoelectrospray Tandem Mass Spectrometry and Sequence -- Similarity Searching for Identification of Proteins -- from Organisms with Unknown Genomes -- Anna Shevchenko, Shamil Sunyaev, Adam Liska, Peer Bork, -- and Andrej Shevchenko -- 20 Direct Identification of Proteins in Ultracomplex Mixtures: -- Applications to Proteome Analysis -- David M. Schieltz and John R. Yates, 111 -- 21 Identification of PTH-Amino Acids by HPLC -- Gregory A. Grant and Mark W. Crankshaw -- 22 Protein Sequencer Maintenance and Troubleshooting -- Bryan Dunbar -- 23 N-Terminal Protein Sequencing for Special Applications -- Philip J. Jackson -- 24 Identification of Phosphorylation Sites by Edman Degradation -- Laurey Steinke and Richard G. Cook -- 25 Validation of Protein Sequencing in a Regulated Laboratory -- Philip J. Jackson and Neil Dodsworth -- 26 Automated C-Terminal Protein Sequence Analysis -- Using the Alkylated-Thiohydantoin Method -- David R. Dupont, Sylvia W. Yuen, and Kenneth S. Graham -- 27 Chemical Modifications of Proteins as an Aid to Sequence Analysis -- Alex F. Carne -- 28 Deblocking of N-Terminally Modified Proteins -- Hisashi Hirano and Roza M. Kamp -- 29 Deblocking of Proteins Containing N-Terminal Pyroglutamic Acid -- Jacek Mozdzanowski -- 30 Identification of Sites of Glycosylation -- David J. Harvey -- 31 Analysis of Sites of Protein Phosphorylation -- Alastair Aitken and Michele Learmonth -- 32 Quantitation and Location of Disulfide Bonds in Proteins -- Alastair Aitken and Michele Learmonth -- 33 Getting the Most from Your Protein Sequence -- Richard R. Copley and Robert B. Russell -- Appendix 1: Letter Codes, Structures, Masses, and Derivatives -- of Amino Acids -- James P. Turner -- Appendix 2: Protein Consensus Sequence Motifs -- Alastair Aitken.

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