Drugs of abuse : neurological reviews and protocols / edited by John Q. Wang.

Contributor(s): Wang, John Q
Material type: TextTextSeries: Methods in molecular medicine: 79.Publisher: Totowa, N.J. : Humana Press, ©2003Description: 1 online resource (xiii, 514 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592593583; 1588290573; 9781588290571; 1592593585; 1280842385; 9781280842382; 9786610842384; 6610842388Subject(s): Drugs of abuse -- Laboratory manuals | Molecular pharmacology -- Laboratory manuals | Experimental psychopharmacology -- Laboratory manuals | Microarray Analysis | Molecular Probe Techniques | Nervous System | Biological Science Disciplines | Sequence Analysis | Pharmaceutical Preparations | Neurologic Manifestations | Genetic Phenomena | Investigative Techniques | Behavior and Behavior Mechanisms | Nucleic Acid Hybridization | Genetic Techniques | Natural Science Disciplines | Signs and Symptoms | Microchip Analytical Procedures | Anatomy | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Chemicals and Drugs | Psychiatry and Psychology | Nervous System Diseases | Chemistry Techniques, Analytical | Disease | Phenomena and Processes | Pathological Conditions, Signs and Symptoms | Disciplines and Occupations | Oligonucleotide Array Sequence Analysis | Neurobehavioral Manifestations | Methods | Illicit Drugs | Central Nervous System | Gene Expression | Pharmacology | Drogues -- Manuels de laboratoire | Pharmacologie moléculaire -- Manuels de laboratoire | Psychopharmacologie expérimentale -- Manuels de laboratoire | MEDICAL -- Pain Medicine | MEDICAL -- Psychiatry -- Psychopharmacology | Drugs of abuse | Experimental psychopharmacology | Molecular pharmacology | Geneesmiddelen | Centraal zenuwstelsel | Neurofarmacologie | Wetenschappelijke techniekenGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Drugs of abuse.DDC classification: 615/.78/0287 LOC classification: RM316 | .D835 2003Other classification: 44.38 Online resources: Click here to access online
Contents:
Drugs of abuse. Introduction / J.Q. Wang -- Detection of cell proliferation and cell fate in adult CNS using BrdU double-label immunohistochemistry / Z.Z. Khaing and M. Blum -- Conditioned place preference. A simple method for investigating reinforcing properties in laboratory animals / E. Carboni and C. Vacca -- Laboratory analysis of behavioral effects of drugs of abuse in rodents / N.M. Richtand -- Measurement of dopamine uptake in neuronal cells and tissues / Y.S. Lau -- Real-time measurements of phasic changes in extracellular dopamine concentration in freely moving rats by fast-scan cyclic voltammetry / P.E. Phillips [and others] -- HPLC/EC detection and quantification of acetylcholine in dialysates / J.A. Zackheim and E.D. Abercrombie -- Microdialysis coupled with electrochemical detection. A way to investigate brain monoamine role in freely moving animals / E. Carboni -- Organotypic culture of developing striatum. Pharmacological induction of gene expression / F.C. Liu -- Primary culture of adult neural progenitors / S.A. Bennett and L. Melanson-Drapeau -- Primary rat mesencephalic neuron-glia, neuron-enriched, microglia-enriched, and astroglia-enriched cultures / B. Liu and J.S. Hong -- Primary striatal neuronal culture / L. Mao and J.Q. Wang -- Antisense approaches in analyzing the functional role of proteins in the central nervous system / J.Q. Wang and L. Mao -- Generating gene knockout mice for studying mechanisms underlying drug addiction / J. Zhang and M. Xu -- Viral-mediated gene transfer to study the behavioral correlates of CREB function in the nucleus accumbens of rats / W.A. Carlezon, Jr. and R.L. Neve -- Analysis of DNA-binding activity in neuronal tissue with the electrophoretic mobility-shift assay / C.L. Konradi -- Dopamine receptor binding and quantitative autoradiographic study / B. Levant -- Analysis of protein expression in brain tissue by ELISA / S.A. Bennett and D.C. Roberts -- Immunohistochemical and immunocytochemical detection of phosphoproteins in striatal neurons / L. Mao and J.Q. Wang -- Quantification of protein in brain tissue by western immunoblot analysis / C.L. Konradi -- Systematic screening of gene expression using a cDNA macroarray / T.J. Worst [and others] -- Application of TaqMan RT-PCR for real-time semiquantitative analysis of gene expression in the striatum / A.D. Medhurst and M.N. Pangalos -- Semiquantitative real-time PCR for analysis of mRNA levels / S.J. Walker, T.J. Worst and K.E. Vrana -- Analysis of mRNA expression in striatal tissue by differential display polymerase chain reaction / J.D. Berke -- Analysis of gene expression in striatal tissue by multiprobe RNase protection assay / N.M. Richtand -- Quantification of mRNA in neuronal tissue by northern analysis / C.L. Konradi -- Analysis of mRNA expression using double in situ hybridization labeling with isotopic and nonisotopic probes / J.Q. Wang -- Combining in situ hybridization with retrograde tracing and immunohistochemistry for phenotypic characterization of individual neurons / C. Le Moine -- In situ hybridization with isotopic riboprobes for detection of striatal neuropeptide mRNA expression after dopamine stimulant administration / Y.L. Hurd -- From drugs of abuse to parkinsonism. The MPTP mouse model of Parkinson's disease / Y.S. Lau and G.E. Meredith -- Learning and memory mechanisms involved in compulsive drug use and relapse / J.D. Berke -- Neuropharmacology and neurotoxicity of 3,4-methylenedioxymethamphetamine / G.A. Gudelsky and B.K. Yamamoto -- Neuroprotective effect of naloxone in inflammation-mediated dopaminergic neurodegeneration. Dissociation from the involvement of opioid receptors / B. Liu and J.S. Hong -- Adult neural stem/progenitor cells in the forebrain. Implications for psychostimulant dependence and medication / J.Q. Wang, L. Mao and Y.S. Lau -- Effects of psychomotor stimulants on glutamate receptor expression / M.E. Wolf -- The temporal sequence of changes in gene expression by drugs of abuse / P.W. Kalivas [and others].
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: New molecular technologies have transformed our understanding of the neurological impact of drugs of abuse and opened many new possibilities for research. In Drugs of Abuse: Neurological Reviews and Protocols, leading scientists from all over the world review these novel research areas and provide a cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Additional methods are provided for primary neural cell culture preparation, for an immunohistochemical method to examine cellular proliferation and differentiation, and for a culture method to grow viable neural progenitors. All contributors have considerable experience with the protocols covered, and each protocol has been thoroughly tried and successfully tested in the laboratory. Helpful notes for each method discuss tricks-of-the-trade, pitfalls to avoid, and troubleshooting. State-of-the-art and highly practical, Drugs of Abuse: Neurological Reviews and Protocols offers insightful reviews of key current topics and a core collection of readily reproducible techniques essential for any neurobiological laboratory carrying out either basic analyses or seeking novel research approaches.
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Includes bibliographical references and index.

Drugs of abuse. Introduction / J.Q. Wang -- Detection of cell proliferation and cell fate in adult CNS using BrdU double-label immunohistochemistry / Z.Z. Khaing and M. Blum -- Conditioned place preference. A simple method for investigating reinforcing properties in laboratory animals / E. Carboni and C. Vacca -- Laboratory analysis of behavioral effects of drugs of abuse in rodents / N.M. Richtand -- Measurement of dopamine uptake in neuronal cells and tissues / Y.S. Lau -- Real-time measurements of phasic changes in extracellular dopamine concentration in freely moving rats by fast-scan cyclic voltammetry / P.E. Phillips [and others] -- HPLC/EC detection and quantification of acetylcholine in dialysates / J.A. Zackheim and E.D. Abercrombie -- Microdialysis coupled with electrochemical detection. A way to investigate brain monoamine role in freely moving animals / E. Carboni -- Organotypic culture of developing striatum. Pharmacological induction of gene expression / F.C. Liu -- Primary culture of adult neural progenitors / S.A. Bennett and L. Melanson-Drapeau -- Primary rat mesencephalic neuron-glia, neuron-enriched, microglia-enriched, and astroglia-enriched cultures / B. Liu and J.S. Hong -- Primary striatal neuronal culture / L. Mao and J.Q. Wang -- Antisense approaches in analyzing the functional role of proteins in the central nervous system / J.Q. Wang and L. Mao -- Generating gene knockout mice for studying mechanisms underlying drug addiction / J. Zhang and M. Xu -- Viral-mediated gene transfer to study the behavioral correlates of CREB function in the nucleus accumbens of rats / W.A. Carlezon, Jr. and R.L. Neve -- Analysis of DNA-binding activity in neuronal tissue with the electrophoretic mobility-shift assay / C.L. Konradi -- Dopamine receptor binding and quantitative autoradiographic study / B. Levant -- Analysis of protein expression in brain tissue by ELISA / S.A. Bennett and D.C. Roberts -- Immunohistochemical and immunocytochemical detection of phosphoproteins in striatal neurons / L. Mao and J.Q. Wang -- Quantification of protein in brain tissue by western immunoblot analysis / C.L. Konradi -- Systematic screening of gene expression using a cDNA macroarray / T.J. Worst [and others] -- Application of TaqMan RT-PCR for real-time semiquantitative analysis of gene expression in the striatum / A.D. Medhurst and M.N. Pangalos -- Semiquantitative real-time PCR for analysis of mRNA levels / S.J. Walker, T.J. Worst and K.E. Vrana -- Analysis of mRNA expression in striatal tissue by differential display polymerase chain reaction / J.D. Berke -- Analysis of gene expression in striatal tissue by multiprobe RNase protection assay / N.M. Richtand -- Quantification of mRNA in neuronal tissue by northern analysis / C.L. Konradi -- Analysis of mRNA expression using double in situ hybridization labeling with isotopic and nonisotopic probes / J.Q. Wang -- Combining in situ hybridization with retrograde tracing and immunohistochemistry for phenotypic characterization of individual neurons / C. Le Moine -- In situ hybridization with isotopic riboprobes for detection of striatal neuropeptide mRNA expression after dopamine stimulant administration / Y.L. Hurd -- From drugs of abuse to parkinsonism. The MPTP mouse model of Parkinson's disease / Y.S. Lau and G.E. Meredith -- Learning and memory mechanisms involved in compulsive drug use and relapse / J.D. Berke -- Neuropharmacology and neurotoxicity of 3,4-methylenedioxymethamphetamine / G.A. Gudelsky and B.K. Yamamoto -- Neuroprotective effect of naloxone in inflammation-mediated dopaminergic neurodegeneration. Dissociation from the involvement of opioid receptors / B. Liu and J.S. Hong -- Adult neural stem/progenitor cells in the forebrain. Implications for psychostimulant dependence and medication / J.Q. Wang, L. Mao and Y.S. Lau -- Effects of psychomotor stimulants on glutamate receptor expression / M.E. Wolf -- The temporal sequence of changes in gene expression by drugs of abuse / P.W. Kalivas [and others].

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New molecular technologies have transformed our understanding of the neurological impact of drugs of abuse and opened many new possibilities for research. In Drugs of Abuse: Neurological Reviews and Protocols, leading scientists from all over the world review these novel research areas and provide a cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Additional methods are provided for primary neural cell culture preparation, for an immunohistochemical method to examine cellular proliferation and differentiation, and for a culture method to grow viable neural progenitors. All contributors have considerable experience with the protocols covered, and each protocol has been thoroughly tried and successfully tested in the laboratory. Helpful notes for each method discuss tricks-of-the-trade, pitfalls to avoid, and troubleshooting. State-of-the-art and highly practical, Drugs of Abuse: Neurological Reviews and Protocols offers insightful reviews of key current topics and a core collection of readily reproducible techniques essential for any neurobiological laboratory carrying out either basic analyses or seeking novel research approaches.

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