Bacterial toxins : methods and protocols / edited by Otto Holst.
Contributor(s): Holst, OttoMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 145.Publisher: Totowa, N.J. : Humana Press, ©2000Description: 1 online resource (xi, 379 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592590520; 0896036049; 9780896036048; 1592590527Subject(s): Bacterial toxins -- Research -- Methodology | Bacterial Toxins | Research Design | SCIENCE -- Life Sciences -- Biology | SCIENCE -- Life Sciences -- Microbiology | Life sciences | Microbiology | Vergif | Bacteriën | Wetenschappelijke techniekenGenre/Form: Electronic books. Additional physical formats: Print version:: Bacterial toxins.DDC classification: 579.3/165 LOC classification: QP632.B3 | B334 2000Other classification: 44.43 Online resources: Click here to access online
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Includes bibliographical references and index.
Bacterial protein toxins: an overview -- Purification of clostridium botulinum type A neurotoxin -- Shiga toxins -- Isolation and detection of microcystins and nodularins, cyanobacterial peptide hepatotoxins -- Genetic construction, expression, and characterization of diphtheria toxin-based growth factor fusion proteins -- Structure-function analysis of cysteine-engineered entomopathogenic toxins -- Use of fourier-transformed infrared spectroscopy for secondary structrue determination of staphyloccoccal pore-forming toxins -- The use of fluorescence resonance energy transfer to detect conformational changes in protein toxins -- Site-directed spin labeling of proteins: applications to diphtheria toxin -- Characterization of molecular properties of pore-forming toxins with planar lipid bilayers -- Determination of affinity and kinetic rate constants using surface plasmon resonance -- ADP-ribosylation of [alpha]-Gi proteins by pertusis toxin: positional dissection of acceptor sites using membrane anchored synthetic peptides -- Phage libraries for generation of anti-botulinum scFv antibiodies -- T-cell cytotoxicity assays for studying the functional itneraction between the superantigen staphylococcal enterotoxin A and T-cell receptors -- In vitro physiological studies on clostridial neurotoxins: biological modesl and procedures for extracellular and intracellular application of toxins -- The biology of endotoxin -- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of lipopolysaccharides -- Applications of combined capillary electrophoresis-electrospray mass spectrometry in the characterization of short-chain lipopolysaccharides: haemophilus influenzae -- Deacylation of lipopolysaccharides and isolation of oligosaccharide phosphates -- Electrophysiological measurement on reconstituted outer membranes.
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Experienced researchers describe in step-by-step detail powerful methods for the investigation of bacterial exo- and endotoxins. These state-of-the-art techniques range from purification and detection methods to methods of conformational analysis, and include the use of phage antibody libraries and methods of structural and functional analyses of endotoxins. Each protocol is described by a scientist who has regularly used the method and optimized it to a high level of performance. Extensive notes deal with the difficulties that may arise when using the method and with the method's limitations and possible modification for other purposes. Cutting-edge and geared to producing successful experimental results, Bacterial Toxins: Methods and Protocols provides investigators with a first-rate collection of readily reproducible methods designed to help today's bacterial toxin investigators-both novice and expert-utilize a wide array of powerful research tools.