Antibiotic resistance : methods and protocols / edited by Stephen H. Gillespie.

Contributor(s): Gillespie, S. H
Material type: TextTextSeries: Methods in molecular medicine: 48.Publisher: Totowa, N.J. : Humana Press, 2001Description: 1 online resource (xv, 287 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592590773; 1592590772Other title: Antibiotic resistence [Available from some providers with the title:]Subject(s): Drug resistance in microorganisms -- Laboratory manuals | Antibiotics -- Laboratory manuals | Drug Resistance, Microbial -- physiology | Anti-Bacterial Agents -- therapeutic use | Anti-Infective Agents -- therapeutic use | Clinical Protocols | Micro-organismes -- Résistance aux médicaments -- Manuels de laboratoire | Antibiotiques -- Manuels de laboratoire | MEDICAL -- Microbiology | Antibiotics | Drug resistance in microorganisms | Antibiotikum | Molekulare Medizin | Resistenz | Wetenschappelijke technieken | Antibiotiques -- Manuels de laboratoire | Microorganismes -- Résistance aux médicaments -- Manuels de laboratoire | Résistance aux médicaments | antibiotica | antibiotics | weerstand | resistance | bacteriologie | bacteriology | microbiologie | microbiology | moleculaire biologie | molecular biology | biologische technieken | biological techniques | antibioticaresistentie | antibiotic resistance | genexpressieanalyse | genomics | protocollen | protocols | Bacteriology | Molecular Biology (General) | Bacteriologie | Moleculaire biologie (algemeen)Genre/Form: Electronic books. | Fulltext. | Internet Resource. | Laboratory Manuals. | Index not Present. | Handbooks and manuals. | Electronic books. Additional physical formats: Print version:: Antibiotic resistance.DDC classification: 616/.01 LOC classification: QR177 | .A564 2001Other classification: XI 3511 Online resources: Click here to access online
Contents:
Multiplex polymerase chain reaction detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci / R. Patel, J.R. Uhl and F.R. Cockerill -- Drug susceptibility of mycobacterium tuberculosis through the mycolic acid index / J.M. Viader-Salvadó [and others] -- Micro-well phage replication assay for screening mycobacteria for resistance to rifampin and streptomycin / R. McNerney -- Application of SSCP to identification of resistance mutations / T.D. McHugh -- Quantitative, single-tube, nested, PCR (QSTN-PCR) for determining the antibiotic susceptibility of mycobacterium tuberculosis / N.-T. Jou and M.R. Liebling -- Rapid rifamycin susceptibility testing of small-volume mycrobacterium tuberculosis cultures by detection of precursor rrna / B.H. William and C.A. Gerard -- Detection of penicillin resistance in Streptococcus pneumoniae by a seminested PCR strategy / M.d. Plessis, A.M. Smith and K.P. Klugman -- Diagnosis of penicillin resistance by PCR-RFLP / G.C. Whiting -- Detection methods of glycopeptide-resistant Staphylococcus aureus i / H. Hanaki and K. Hiramatsu -- Detection methods for glycopeptide-resistant Staphylococcus aureus ii / H. Hanaki and K. Hiramatsu -- Multiplex PCR for the rapid simultaneous speciation and detection of methicillin-resistance and genes encoding toxin production in Staphylococcus aureus / M.E. Jones, K. Köhrer and F.-J. Schmitz -- Chromogenic detection of aminoglycoside phosphotransferases / A.M. Amoroso and G.O. Gutkind -- Quantification of m. Tuberculosis DNA in sputum during the treatment of pulmonary tuberculosis / L.E. DesJardin and G.L. Hehman -- Isolation of m. Tuberculosis RNA from sputum / L.E. DesJardin -- Detection of viable mycobacterium tuberculosis by reverse transcriptase-strand displacement amplification of mRNA / H.J. Tobin -- Box PCR fingerprinting for molecular typing of Streptococcus pneumoniae / A. Belkum and P.W.M. Hermans -- Restriction fragment end labeling analysis: High-resolution genomic typing of Streptococcus pneumoniae isolates / P.W.M. Hermans, M. Sluijter and A. Belkum -- Pulse-field gel electrophoresis for epidemiological studies of Streptococcus pneumoniae / A.-M. Gasc -- Outer membrane profiles of clonally related klebsiella pneumoniae / B.J. Vicente and M.-M. Luis -- Atomic force microscopy / P.C. Braga and D. Ricci -- Assessing the activity of bacterial multidrug efflux pumps / K. Poole and R. Srikumar -- The use of a continuous culture system to study the antimicrobial susceptibility of bacteria in biofilm / J.K. Struthers -- Estimation of mutation rates in antibiotic research / O.J. Billington and S.H. Gillespie -- In vitro assessment of the fitness of resistant m. Tuberculosis bacteria by competition assay / S.H. Gillespie -- Purification of DNA topoisomerases and inhibition by fluoroquinolones / G.T. John and M. Ian -- Site-directed mutagenesis to determine structure function relationships in Streptococcus pneumoniae penicillin-binding protein genes / V.A. Barcus and C.G. Dowson -- Detection of low affinity penicillin-binding protein variants in Streptococcus pneumoniae / R. Hakenbeck -- Mobilization of transposons / L.B. Rice.
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: At a time of rising concern about drug resistance and falling output of new antibacterial compounds, antibiotic research has once again returned to the forefront of medical science. In Antibiotic Resistance: Methods and Protocols, Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. The methods are readily adaptable to a wide range of resistant bacterial organisms. In order to ensure successful results, each method is described in minute detail and includes tips on avoiding pitfalls. Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools.
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Includes bibliographical references and index.

Multiplex polymerase chain reaction detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci / R. Patel, J.R. Uhl and F.R. Cockerill -- Drug susceptibility of mycobacterium tuberculosis through the mycolic acid index / J.M. Viader-Salvadó [and others] -- Micro-well phage replication assay for screening mycobacteria for resistance to rifampin and streptomycin / R. McNerney -- Application of SSCP to identification of resistance mutations / T.D. McHugh -- Quantitative, single-tube, nested, PCR (QSTN-PCR) for determining the antibiotic susceptibility of mycobacterium tuberculosis / N.-T. Jou and M.R. Liebling -- Rapid rifamycin susceptibility testing of small-volume mycrobacterium tuberculosis cultures by detection of precursor rrna / B.H. William and C.A. Gerard -- Detection of penicillin resistance in Streptococcus pneumoniae by a seminested PCR strategy / M.d. Plessis, A.M. Smith and K.P. Klugman -- Diagnosis of penicillin resistance by PCR-RFLP / G.C. Whiting -- Detection methods of glycopeptide-resistant Staphylococcus aureus i / H. Hanaki and K. Hiramatsu -- Detection methods for glycopeptide-resistant Staphylococcus aureus ii / H. Hanaki and K. Hiramatsu -- Multiplex PCR for the rapid simultaneous speciation and detection of methicillin-resistance and genes encoding toxin production in Staphylococcus aureus / M.E. Jones, K. Köhrer and F.-J. Schmitz -- Chromogenic detection of aminoglycoside phosphotransferases / A.M. Amoroso and G.O. Gutkind -- Quantification of m. Tuberculosis DNA in sputum during the treatment of pulmonary tuberculosis / L.E. DesJardin and G.L. Hehman -- Isolation of m. Tuberculosis RNA from sputum / L.E. DesJardin -- Detection of viable mycobacterium tuberculosis by reverse transcriptase-strand displacement amplification of mRNA / H.J. Tobin -- Box PCR fingerprinting for molecular typing of Streptococcus pneumoniae / A. Belkum and P.W.M. Hermans -- Restriction fragment end labeling analysis: High-resolution genomic typing of Streptococcus pneumoniae isolates / P.W.M. Hermans, M. Sluijter and A. Belkum -- Pulse-field gel electrophoresis for epidemiological studies of Streptococcus pneumoniae / A.-M. Gasc -- Outer membrane profiles of clonally related klebsiella pneumoniae / B.J. Vicente and M.-M. Luis -- Atomic force microscopy / P.C. Braga and D. Ricci -- Assessing the activity of bacterial multidrug efflux pumps / K. Poole and R. Srikumar -- The use of a continuous culture system to study the antimicrobial susceptibility of bacteria in biofilm / J.K. Struthers -- Estimation of mutation rates in antibiotic research / O.J. Billington and S.H. Gillespie -- In vitro assessment of the fitness of resistant m. Tuberculosis bacteria by competition assay / S.H. Gillespie -- Purification of DNA topoisomerases and inhibition by fluoroquinolones / G.T. John and M. Ian -- Site-directed mutagenesis to determine structure function relationships in Streptococcus pneumoniae penicillin-binding protein genes / V.A. Barcus and C.G. Dowson -- Detection of low affinity penicillin-binding protein variants in Streptococcus pneumoniae / R. Hakenbeck -- Mobilization of transposons / L.B. Rice.

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At a time of rising concern about drug resistance and falling output of new antibacterial compounds, antibiotic research has once again returned to the forefront of medical science. In Antibiotic Resistance: Methods and Protocols, Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. The methods are readily adaptable to a wide range of resistant bacterial organisms. In order to ensure successful results, each method is described in minute detail and includes tips on avoiding pitfalls. Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools.

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