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DNA sequencing protocols / edited by Colin A. Graham and Allison J.M. Hill.

Contributor(s): Graham, Colin A | Hill, Alison J. M
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 167.Publisher: Totowa, NJ : Humana Press, ©2001Edition: 2nd edDescription: 1 online resource (x, 244 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781592591138; 1592591132Subject(s): Nucleotide sequence -- Laboratory manuals | Sequence Analysis, DNA | Séquence nucléotidique -- Manuels de laboratoire | ADN -- Manuels de laboratoire | SCIENCE -- Life Sciences -- Genetics & Genomics | Nucleotide sequence | Wetenschappelijke technieken | dna sequencing | nucleotidenvolgordes | nucleotide sequences | dna | biologische technieken | biological techniques | protocollen | protocols | Nucleic AcidsGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: DNA sequencing protocols.DDC classification: 572.8/633 LOC classification: QP624 | .D634 2001ebOnline resources: Click here to access online
Contents:
Introduction to DNA sequencing / Colin A. Graham, Alison J.M. Hill -- The universal primers and the shotgun DNA sequencing method / Joachim Messing -- M13 sequencing / Qingzhong Yu -- Primer design and primer-directed sequencing / Ulrike Gerischer, Peter Durre -- Direct sequencing of DNA produced in a polymerase chain reaction / Ulrike Gerischer -- Solid phase flluorescent sequencing of the CFTR gene / Harry Cuppens, Jean-Jacques Cassiman -- Shotgun DNA sequencing / Alan T. Bankier -- Cycle sequencing / Robert W. Blakesley -- Cycle sequencing of polymerase chain reaction-amplified genomic DNA with dye-labeled universal primers / Johannes Zschocke, Georg F. Hoffman -- Automated fluorescent DNA sequencing on the ABI PRISM 377 / Jonathan R.E. MacBeath, Suzanna S. Harvey, Nicola J. Oldroyd -- Automated fluorescent DNA sequencing on the ABI PRISM 310 genetic analyzer / David Watts, Jonathan R.E. MacBeath -- Fluorescent sequencing protocols for the ALF / Shirley McQuaid -- Fluorescent sequencing for heterozygote mutation detection / Colin A. Graham, Alison J.M. Hill -- Sequence databases and the Internet / Anne E. Hughes -- DNA sequencing by capillary array electrophoresis / Norman J. Dovichi, JianZhong Zhang.
Summary: Annotation Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.
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Includes bibliographical references and index.

Introduction to DNA sequencing / Colin A. Graham, Alison J.M. Hill -- The universal primers and the shotgun DNA sequencing method / Joachim Messing -- M13 sequencing / Qingzhong Yu -- Primer design and primer-directed sequencing / Ulrike Gerischer, Peter Durre -- Direct sequencing of DNA produced in a polymerase chain reaction / Ulrike Gerischer -- Solid phase flluorescent sequencing of the CFTR gene / Harry Cuppens, Jean-Jacques Cassiman -- Shotgun DNA sequencing / Alan T. Bankier -- Cycle sequencing / Robert W. Blakesley -- Cycle sequencing of polymerase chain reaction-amplified genomic DNA with dye-labeled universal primers / Johannes Zschocke, Georg F. Hoffman -- Automated fluorescent DNA sequencing on the ABI PRISM 377 / Jonathan R.E. MacBeath, Suzanna S. Harvey, Nicola J. Oldroyd -- Automated fluorescent DNA sequencing on the ABI PRISM 310 genetic analyzer / David Watts, Jonathan R.E. MacBeath -- Fluorescent sequencing protocols for the ALF / Shirley McQuaid -- Fluorescent sequencing for heterozygote mutation detection / Colin A. Graham, Alison J.M. Hill -- Sequence databases and the Internet / Anne E. Hughes -- DNA sequencing by capillary array electrophoresis / Norman J. Dovichi, JianZhong Zhang.

Annotation Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will work and impact today's genome sequencing projects.

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