Germ cell protocols. Part 1, Sperm and oocyte analysis / edited by Heide Schatten.

Contributor(s): Schatten, Heide
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): volume 253, pt. 1.Publisher: Totowa, N.J. : Humana Press, ©2004Description: 1 online resource (1 volume) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 1592597440; 9781592597444Other title: Sperm and oocyte analysisSubject(s): Veterinary embryology -- Research -- Technique | Germ cells -- Research -- Technique | Cytological Techniques | Germ Cells | Genetic Techniques | MEDICAL -- Veterinary Medicine -- Food Animal | kiemcellen | germ cells | spermatozoa | oocytes | laboratoriummethoden | laboratory methods | technieken | techniques | rijpen | maturation | in vitro bevruchting | in vitro fertilization | genetische analyse | genetic analysis | celbiologie | cellular biology | moleculaire biologie | molecular biology | protocollen | protocols | Reproduction and Fertility | Cellular Biology | Voortplanting en vruchtbaarheid | CelbiologieGenre/Form: Electronic books. Additional physical formats: Print version:: Germ cell protocols. Volume 1, Sperm and oocyte analysis.DDC classification: 636.089/264 LOC classification: SF767.5Online resources: Click here to access online
Contents:
Cryopreservation of semen of the salmonidae with special reference to large-scale fertilization / F. Lahnsteiner -- Fertilization and sperm chemotaxis in ascidians / M. Yoshida -- Assays for vertebrate sperm chemotaxis / H. Sugiyama [and others] -- Stallion spermatozoa viability: Comparison of flow cytometry with other methods / K. Merkies and M.M. Buhr -- Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa, and zygotes by immunofluorescence, confocal, and immunoelectron microscopy / P. Sutovsky -- Localization of zona pellucida receptors on live sperm by fluorophore-conjugated solubilized zona pellucida proteins / H.R. Burkin, A. Paula Alves-Vieira and D.J. Miller -- Detection of human papillomavirus DNA in sperm using polymerase chain reaction / O.A. Olatunbosun, A.M. Case and H.G. Deneer -- Spermatogonial stem cells / K. Nayernia, M. Li and W. Engel -- Molecular and cellular approaches for establishment of an in vitro spermatogenesis system / K. Nayernia and W. Engel -- Acrosin activity assay for the evaluation of mammalian sperm acrosome reaction / Y. Lax, S. Rubinstein and H. Breitbart -- Isolation of sea urchin sperm plasma membranes / K.J. Mengerink and V.D. Vacquier -- Detection of centrosome structure in fertilized and artificially activated sea urchin eggs using immunofluorescence microscopy and isolation of centrosomes followed by structural characterization with field emission scanning electron microscopy / H. Schatten and A. Chakrabarti -- Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development / M.W. Tengowski -- Immunocytochemical staining of the metaphase ii spindle in mammalian oocytes / S.F. Mullen and J.K. Critser -- Spindle imaging in living mammalian oocytes using the liquid-crystal polarized light microscope and its practical use / W.H. Wang, J. Gill and C. Boutin -- In vitro maturation and fertilization of pig oocytes / H.Y. Fan and Q.Y. Sun -- In vitro maturation and in vitro fertilization of human oocytes / W.H. Wang and J. Gill -- In vitro maturation and fertilization of canine oocytes / K. Reynaud, M. Saint-Dizier and S. Chastant-Maillard -- Detection and measurement of membrane-bound protein tyrosine kinases in the zebrafish egg / W. Wu and W.H. Kinsey -- Single-cell method for estimation of protein tyrosine kinases in the zebrafish egg / E. MacGregor and W.H. Kinsey -- Activity of MAPK/p90rsk during fertilization in mice, rats, and pigs / Q.Y. Sun and H.Y. Fan.
Abstract: A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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Includes bibliographical references and indexes.

Cryopreservation of semen of the salmonidae with special reference to large-scale fertilization / F. Lahnsteiner -- Fertilization and sperm chemotaxis in ascidians / M. Yoshida -- Assays for vertebrate sperm chemotaxis / H. Sugiyama [and others] -- Stallion spermatozoa viability: Comparison of flow cytometry with other methods / K. Merkies and M.M. Buhr -- Visualization of sperm accessory structures in the mammalian spermatids, spermatozoa, and zygotes by immunofluorescence, confocal, and immunoelectron microscopy / P. Sutovsky -- Localization of zona pellucida receptors on live sperm by fluorophore-conjugated solubilized zona pellucida proteins / H.R. Burkin, A. Paula Alves-Vieira and D.J. Miller -- Detection of human papillomavirus DNA in sperm using polymerase chain reaction / O.A. Olatunbosun, A.M. Case and H.G. Deneer -- Spermatogonial stem cells / K. Nayernia, M. Li and W. Engel -- Molecular and cellular approaches for establishment of an in vitro spermatogenesis system / K. Nayernia and W. Engel -- Acrosin activity assay for the evaluation of mammalian sperm acrosome reaction / Y. Lax, S. Rubinstein and H. Breitbart -- Isolation of sea urchin sperm plasma membranes / K.J. Mengerink and V.D. Vacquier -- Detection of centrosome structure in fertilized and artificially activated sea urchin eggs using immunofluorescence microscopy and isolation of centrosomes followed by structural characterization with field emission scanning electron microscopy / H. Schatten and A. Chakrabarti -- Microscopic techniques for studying sperm-oocyte interaction during fertilization and early embryonic development / M.W. Tengowski -- Immunocytochemical staining of the metaphase ii spindle in mammalian oocytes / S.F. Mullen and J.K. Critser -- Spindle imaging in living mammalian oocytes using the liquid-crystal polarized light microscope and its practical use / W.H. Wang, J. Gill and C. Boutin -- In vitro maturation and fertilization of pig oocytes / H.Y. Fan and Q.Y. Sun -- In vitro maturation and in vitro fertilization of human oocytes / W.H. Wang and J. Gill -- In vitro maturation and fertilization of canine oocytes / K. Reynaud, M. Saint-Dizier and S. Chastant-Maillard -- Detection and measurement of membrane-bound protein tyrosine kinases in the zebrafish egg / W. Wu and W.H. Kinsey -- Single-cell method for estimation of protein tyrosine kinases in the zebrafish egg / E. MacGregor and W.H. Kinsey -- Activity of MAPK/p90rsk during fertilization in mice, rats, and pigs / Q.Y. Sun and H.Y. Fan.

Print version record.

A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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