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Platelets and megakaryocytes. Volume 2, Perspectives and techniques / edited by Jonathan M. Gibbins, Martyn P. Mahaut-Smith.

Contributor(s): Gibbins, Jonathan M | Mahaut-Smith, Martyn PMaterial type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 273.Publication details: Totowa, N.J. : Humana Press, ©2004. Description: 1 online resource (1 volume) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592597833; 1592597831; 1588290115; 9781588290113; 1280360186; 9781280360183; 9786610360185; 6610360189Subject(s): Blood platelets -- Laboratory manuals | Megakaryocytes -- Laboratory manuals | Blood Platelets -- physiology | Histological Techniques | Megakaryocytes -- physiology | MEDICAL -- Physiology | SCIENCE -- Life Sciences -- Human Anatomy & Physiology | Blood platelets | Megakaryocytes | Plaquetas sanguíneas | bloedplaatjes | platelets | beenmergcellen | bone marrow cells | bloedcellen | blood cells | receptoren | receptors | biologische technieken | biological techniques | moleculaire biologie | molecular biology | protocollen | protocols | Histology | Molecular Biology (General) | Histologie | Moleculaire biologie (algemeen)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Platelets and megakaryocytes. Volume 2, Perspectives and techniques.DDC classification: 612.1/17 LOC classification: QP97 | .P568eb vol. 2NLM classification: W1 | WH 300Online resources: Click here to access online
Contents:
Signaling receptors on platelets and megakaryocytes / D. Woulfe [and others] -- Manipulation of gene expression in megakaryocytes / N.R. Emambokus, G.J. Murphy and J. Frampton -- In vitro changes of platelet parameters: Lessons from blood banking / J. Rivera, M.L. Lozano and V. Vicente -- Pharmacological approaches to studying platelet function: An overview / S.M. Hourani -- Inhibitors of cellular signaling targets: Designs and limitations / C.J. Vlahos and L.F. Stancato -- Ligand-binding assays for collagen / S.M. Jung and M. Moroi -- Use of radiolabeled 2-methylthio-adp to study p2y receptors on platelets and cell lines / P. Savi and J.M. Herbert -- Ligand-binding assays: Fibrinogen / D. Cox -- Techniques for analysis of proteins by sds-polyacrylamide gel electrophoresis and western blotting / J.M. Gibbins -- Study of tyrosine kinases and protein tyrosine phosphorylation / J.M. Gibbins -- Protein tyrosine phosphatases / M.L. Jones and A.W. Poole -- The study of serine-threonine kinases / G. Gorter and J. Willem Akkerman -- Phosphoinositides: Lipid kinases and phosphatases / B. Payrastre -- Isolation and analysis of platelet lipid rafts / C.N. Shrimpton [and others] -- Measurement and manipulation of [ca2+]i in suspensions of platelets and cell cultures / P. Ohlmann [and others] -- Measurement and manipulation of intracellular ca2+ in single platelets and megakaryocytes / M.J. Mason and M.P. Mahaut-Smith -- Patch-clamp recordings of electrophysiological events in the platelet and megakaryocyte / M.P. Mahaut-Smith -- Evaluation of nitrotyrosine-containing proteins in blood platelets / K.M. Naseem, R. Riba and M. Troxler -- Nitric oxide signaling in platelets / S.Y. Low and K.R. Bruckdorfer -- Snake venom toxins affecting platelet function / R.K. Andrews, E.E. Gardiner and M.C. Berndt -- Peptide synthesis in the study of collagen-platelet interactions / C.G. Knight, C.M. Onley and R.W. Farndale -- Platelet permeabilization / R. Flaumenhaft -- Using retroviruses to express genes in primary megakaryocyte lineage cells / M. Gaur [and others] -- Use of antisense oligonucleotide technology to investigate signaling pathways in megakaryocytes / H. Avraham, S. Avraham and R. Zagozdzon -- Gfp fusion proteins to study signaling in live cells / S.A. Walker, G.E. Cozier and P.J. Cullen -- Two-dimensional polyacrylamide gel electrophoresis for platelet proteomics / K. Marcus and H.E. Meyer -- Preparation of mrna and cdna libraries from platelets and megakaryocytes / B.Z. Paul, J. Jin and S.P. Kunapuli -- Platelet receptor structures and polymorphisms / T.J. Kunicki, S. Head and D.R. Salomon -- Gene array technology and the study of platelets and megakaryocytes / L.T. Lam and E.H. Bresnick.
Summary: Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques leading expert researchers-many responsible for seminal discoveries in the field-offer basic and advanced techniques for studying cell signaling in the regulation and function of platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts in the regulation of cell signaling, and the measurement of intracellular calcium levels. There are also techniques for the study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. The application of many molecular and post-genomic methods to the study of platelets and megakaryocytes is also described. All protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 1: Functional Assays describes in step-by-step detail basic and advanced techniques for analyzing platelet and megakaryocyte function. Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today
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Includes bibliographical references and index.

Signaling receptors on platelets and megakaryocytes / D. Woulfe [and others] -- Manipulation of gene expression in megakaryocytes / N.R. Emambokus, G.J. Murphy and J. Frampton -- In vitro changes of platelet parameters: Lessons from blood banking / J. Rivera, M.L. Lozano and V. Vicente -- Pharmacological approaches to studying platelet function: An overview / S.M. Hourani -- Inhibitors of cellular signaling targets: Designs and limitations / C.J. Vlahos and L.F. Stancato -- Ligand-binding assays for collagen / S.M. Jung and M. Moroi -- Use of radiolabeled 2-methylthio-adp to study p2y receptors on platelets and cell lines / P. Savi and J.M. Herbert -- Ligand-binding assays: Fibrinogen / D. Cox -- Techniques for analysis of proteins by sds-polyacrylamide gel electrophoresis and western blotting / J.M. Gibbins -- Study of tyrosine kinases and protein tyrosine phosphorylation / J.M. Gibbins -- Protein tyrosine phosphatases / M.L. Jones and A.W. Poole -- The study of serine-threonine kinases / G. Gorter and J. Willem Akkerman -- Phosphoinositides: Lipid kinases and phosphatases / B. Payrastre -- Isolation and analysis of platelet lipid rafts / C.N. Shrimpton [and others] -- Measurement and manipulation of [ca2+]i in suspensions of platelets and cell cultures / P. Ohlmann [and others] -- Measurement and manipulation of intracellular ca2+ in single platelets and megakaryocytes / M.J. Mason and M.P. Mahaut-Smith -- Patch-clamp recordings of electrophysiological events in the platelet and megakaryocyte / M.P. Mahaut-Smith -- Evaluation of nitrotyrosine-containing proteins in blood platelets / K.M. Naseem, R. Riba and M. Troxler -- Nitric oxide signaling in platelets / S.Y. Low and K.R. Bruckdorfer -- Snake venom toxins affecting platelet function / R.K. Andrews, E.E. Gardiner and M.C. Berndt -- Peptide synthesis in the study of collagen-platelet interactions / C.G. Knight, C.M. Onley and R.W. Farndale -- Platelet permeabilization / R. Flaumenhaft -- Using retroviruses to express genes in primary megakaryocyte lineage cells / M. Gaur [and others] -- Use of antisense oligonucleotide technology to investigate signaling pathways in megakaryocytes / H. Avraham, S. Avraham and R. Zagozdzon -- Gfp fusion proteins to study signaling in live cells / S.A. Walker, G.E. Cozier and P.J. Cullen -- Two-dimensional polyacrylamide gel electrophoresis for platelet proteomics / K. Marcus and H.E. Meyer -- Preparation of mrna and cdna libraries from platelets and megakaryocytes / B.Z. Paul, J. Jin and S.P. Kunapuli -- Platelet receptor structures and polymorphisms / T.J. Kunicki, S. Head and D.R. Salomon -- Gene array technology and the study of platelets and megakaryocytes / L.T. Lam and E.H. Bresnick.

Print version record.

Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques leading expert researchers-many responsible for seminal discoveries in the field-offer basic and advanced techniques for studying cell signaling in the regulation and function of platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts in the regulation of cell signaling, and the measurement of intracellular calcium levels. There are also techniques for the study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. The application of many molecular and post-genomic methods to the study of platelets and megakaryocytes is also described. All protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 1: Functional Assays describes in step-by-step detail basic and advanced techniques for analyzing platelet and megakaryocyte function. Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today

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