Calpain methods and protocols / edited by John S. Elce.
Contributor(s): Elce, John SMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 144.Publisher: Totowa, N.J. : Humana Press, ©2000Description: 1 online resource (xviii, 343 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592590506; 0896036324; 9780896036321; 1592590500; 128082042X; 9781280820427; 9786610820429; 6610820422Subject(s): Calpain -- Laboratory manuals | Cysteine Endopeptidases | Cysteine Proteases | Endopeptidases | Peptide Hydrolases | Hydrolases | Enzymes | Enzymes and Coenzymes | Chemicals and Drugs | Calpain | SCIENCE -- Life Sciences -- Biochemistry | Calpain | Wetenschappelijke techniekenGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Calpain methods and protocols.DDC classification: 572/.7516 LOC classification: QP609.C26 | C34 2000Online resources: Click here to access online
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Includes bibliographical references and index.
pt. I. Purification of Components of the Calpain System. 1. Purification of [mu]-Calpain, m-Calpain, and Calpastatin from Animal Tissues / Valery F. Thompson and Darrel E. Goll. 2. A Simple Protocol for Separation and Assay of [mu]-Calpain, m-Calpain, and Calpastatin from Small Tissue Samples / Jan-Olof Karlsson. 3. Purification and Quantification of Calcium-Activated Neutral Proteases I and II and Novel Isoforms From Cultured Osteoblastic Cells by Ion-Exchange Fast Protein Liquid Chromatography / Elsa J.B. Murray, Keyvan Behnam and Mario S. Grisanti / [and others]. 4. Purification of Calpain by Affinity Chromatography on Reactive Red-Agarose or on Casein-Sepharose / Dorothy E. Croall. 5. Affinity Purification of [mu]-Calpain from Erythrocytes on an Immobilized Peptide from the Plasma Membrane Calcium Pump: Some Studies on Erythrocyte [mu]-Calpain / Maurizio Molinari and Ernesto Carafoli. 6. Expression of m-Calpain in Escherichia coli / John S. Elce.
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In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of æ- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains.