Interleukin protocols / edited by Luke A.J. O'Neill and Andrew Bowie.
Contributor(s): O'Neill, Luke A. J | Bowie, AndrewMaterial type: TextSeries: Methods in molecular medicine: 60.Publisher: Totowa, N.J. : Humana Press, ©2001Description: 1 online resource (xv, 443 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592591466; 1592591469; 1280821116; 9781280821110; 9786610821112; 6610821119Subject(s): Interleukins -- Laboratory manuals | Epidemiologic Study Characteristics as Topic | Cytokines | Therapeutics | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Intercellular Signaling Peptides and Proteins | Health Care Evaluation Mechanisms | Proteins | Quality of Health Care | Peptides | Biological Factors | Health Care Quality, Access, and Evaluation | Amino Acids, Peptides, and Proteins | Chemicals and Drugs | Delivery of Health Care | Clinical Protocols | Interleukins | MEDICAL -- Immunology | Interleukins | Interleukines | Wetenschappelijke technieken | Interleukines | interleukinen | interleukins | humane ziekten | human diseases | geneeskunde | medicine | signaaltransductie | signal transduction | polymorfisme | polymorphism | cytokinen | cytokines | protocollen | protocols | Human Pathology | Humane pathologieGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Interleukin protocols.DDC classification: 616.07/9 LOC classification: QR185.8.I56 | I587 2001Online resources: Click here to access online
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Includes bibliographical references and index.
ELISAs and interleukin research -- Elispot technique for assaying interleukins -- Measurement of interleukins by ELISPOT assay with particular application to dual-color analysis (stardust assay) -- Assaying interleukins intracellularly by FACS -- Basic RT-PCR for measurement of cytokine expression -- Quantitative PCR for measurement of cytokine expression -- Single cell RT-PCR for the detection of cytokine mRNA -- Quantitation of cytokine mRNA by flash-type bioluminescence -- Measurement of interleukin mRNA by northern blotting using a nonradioactive detection method -- Quantification of mouse IL-6 and TNF-- [alpha] mRNA using xplore assays -- Measurement of cytokine and chemokine mRNA using nonisotopic multiprobe RNase protection assay -- Isolation of total and bioactive interleukins by immunoaffinity-receptor affinity chromatography -- Detection of cytokines by immunohistochemistry -- Whole blood assays and the influence of circadian rhythmicity on human cytokine measurement -- Assaying interleukin-8 -- Measurement of interleukin-2 by bioassay -- Assaying interleukin-15 -- Assaying interleukin-6 in breast cancer -- Cytokines, stress, and depressive illness -- Measurement of interleukins in cutaneous disorders -- Interleukins in Graves' disease -- Evaluation of cytokines in migraine patients by ELISA -- Assaying interleukins in plasma in the course of acute myocardial infarction -- Measurement of cytokines in peritoneal fluids -- Measurement of cytokines in seminal plasma -- Measurement of cytokines in induced sputum -- Cytokines in blister fluids -- Measurement of cytokines in synovial fluid -- Measurement of cytokines in cerebrospinal fluid -- Detection of cytokines in tears -- Measurement of phosphoinositide 3-kinase activation by interleukins -- Analysis of interleukin-2 signaling using affinity precipitations and polyacrylamide gel electrophoresis -- Applications for green fluorescent protein in cell signaling -- Use of Pichia pastoris for production of recombinant cytokines -- 35analysis of single nucleotide polymorphisms in the interleukin-1B gene using 5' nuclease assays -- Measurement of cytokine and chemokine gene expression patterns using cDNA array.
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Interleukins, a family of proteins that regulate immunity and inflammation throughout the body, are also involved in autoimmune disease (rheumatoid arthritis, multiple sclerosis, and asthma); are implicated in such other conditions as cancer, myocardial infarction, and depression; and have value in the diagnosis and prognosis of many diseases. Luke O'Neill and Andrew Bowie bring together in Interleukin Protocols a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave's disease, migraine, and myocardial infarction. There are also helpful discussions of the difficulties in measuring interleukins in different biological fluids, such as sputum from asthma patients, peritoneal fluids, synovial fluid from arthritic joints, and cerbrospinal fluid from patients with meningitis. Each step-by-step method is described by a hands-on expert and includes notes on how to avoid failure. Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics.