Nitric oxide protocols / edited by Michael A. Titheradge.

Contributor(s): Titheradge, Michael A
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 100.Publisher: Totowa, N.J. : Humana Press, ©1998Description: 1 online resource (xi, 324 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592597499; 1592597491; 0896034704; 9780896034709Subject(s): Nitric oxide -- Metabolism | Nitric oxide -- Synthesis | Nitric Oxide -- isolation & purification | Nitric Oxide -- metabolism | SCIENCE -- Life Sciences -- Biochemistry | Nitric oxide -- Metabolism | Methode | Molekularbiologie | Stickstoffoxide | Stikstofmonoxide | Chemische analyses | stikstofoxide | nitric oxide | enzymactiviteit | enzyme activity | immunohistochemie | immunohistochemistry | Biochemistry | BiochemieGenre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Nitric oxide protocols.DDC classification: 572/.54 LOC classification: QP535.N1 | N557 1998Other classification: 35.71 | 35.79 | 42.13 | WC 4150 Online resources: Click here to access online
Contents:
Enzymology of nitric oxide synthases / Benjamin Hemmens and Bernd Mayer -- Purification of the inducible nitric oxide synthase ; Purification of the constitutive nitric oxide synthase / Edward P. Garvey -- Cloning and expression of human inducible nitric oxide synthase / Mark L. Johnson, Richard A. Shapiro, Timothy R. Billiar -- Cloning and expression of human eNOS and nNos using the baculovirus-insect cell system / Ian G. Charles, Neale Foxwell, Ann Chubb -- Assay of NOS activity by the measurement of conversion of oxyhemoglobin to methemoglobin by NO / Mark Salter and Richard G. Knowles -- Measurement of NOS activity by conversion of radiolabeled arginine to citrulline using ion-exchange separation / Richard G. Knowles and Mark Salter -- Radiochemical measurement of NOS activity by conversion of [¹⁴C]L-arginine to citrulline using HPLC separation / James M. Cunningham and Richard C. Rayne -- The enzymatic measurement of nitrate and nitrite / Michael A. Titheradge -- Determination of NOS activity using cyclic-GMP formation / Suzanne G. Laychock -- Determination of NO with a Clark-type electrode / Kurt Schmidt and Bernd Mayer -- The measurement of NO in biological systems using chemiluminescence / Evangelos D. Michelakis and Stephen L. Archer -- Measurement of NO using electron paramagnetic resonance / S. Tsuyoshi Ohnishi -- Measurement of eNOS and iNOS mRNA expression using reverse transcription polymerase chain reaction / Norbert Reiling, Artur J. Ulmer, Sunna Hauschildt -- Measurement of NOS mRNA by Northern blotting and the ribonuclease-protection assay / Paloma Martín-Sanz and Lisardo Boscá -- Detection of NOS isoforms by Western-blot analysis / Fiona S. Smith and Michael A. Titheradge --Immunohistochemical localization of NOS isoforms / Victoria Cattell and Karen Mosley -- A practical protocol for the demonstration of NOS using in-situ hybridization / G. Michael Taylor and H. Terence Cook -- Use of NO donors in biological systems / Jayne M. Tullett and Daryl D. Rees -- Making and working with peroxynitrite / Roger White [and others] -- The use of NO gas in biological systems / Neil Hogg and B. Kalyanaraman -- A microtiter-plate assay of human NOS isoforms / John Dawson and Richard G. Knowles -- Use of arginine analogs as inhibitors of nitric oxide synthase in rat-aortic rings / Rachel J. Russell -- Measurement of biopterin and the use of inhibitors of biopterin biosynthesis / Kazuyuki Hatakeyama -- A sensitive assay for the enzymatic activity of GTP cyclohydrolase I / Kazuyuki Hatakeyama and Toshie Yoneyama -- Simultaneous measurement of mitochondrial function and NO / Ignacio Lizasoain and María A. Moro -- Release of NO from donor compounds : a mathematical model for calculation of NO concentrations in the presence of oxygen / Kurt Schmidt [and others] -- The determination of nitrotyrosine residues in proteins / Andrew J. Gow [and others] -- Measurement of DNA damage using the comet assay / Steven Thomas [and others] -- Methods for the study of NO-induced apoptosis in cultured cells / Anne C. Loweth and Noel G. Morgan.
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.
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Includes bibliographical references and index.

Enzymology of nitric oxide synthases / Benjamin Hemmens and Bernd Mayer -- Purification of the inducible nitric oxide synthase ; Purification of the constitutive nitric oxide synthase / Edward P. Garvey -- Cloning and expression of human inducible nitric oxide synthase / Mark L. Johnson, Richard A. Shapiro, Timothy R. Billiar -- Cloning and expression of human eNOS and nNos using the baculovirus-insect cell system / Ian G. Charles, Neale Foxwell, Ann Chubb -- Assay of NOS activity by the measurement of conversion of oxyhemoglobin to methemoglobin by NO / Mark Salter and Richard G. Knowles -- Measurement of NOS activity by conversion of radiolabeled arginine to citrulline using ion-exchange separation / Richard G. Knowles and Mark Salter -- Radiochemical measurement of NOS activity by conversion of [¹⁴C]L-arginine to citrulline using HPLC separation / James M. Cunningham and Richard C. Rayne -- The enzymatic measurement of nitrate and nitrite / Michael A. Titheradge -- Determination of NOS activity using cyclic-GMP formation / Suzanne G. Laychock -- Determination of NO with a Clark-type electrode / Kurt Schmidt and Bernd Mayer -- The measurement of NO in biological systems using chemiluminescence / Evangelos D. Michelakis and Stephen L. Archer -- Measurement of NO using electron paramagnetic resonance / S. Tsuyoshi Ohnishi -- Measurement of eNOS and iNOS mRNA expression using reverse transcription polymerase chain reaction / Norbert Reiling, Artur J. Ulmer, Sunna Hauschildt -- Measurement of NOS mRNA by Northern blotting and the ribonuclease-protection assay / Paloma Martín-Sanz and Lisardo Boscá -- Detection of NOS isoforms by Western-blot analysis / Fiona S. Smith and Michael A. Titheradge --Immunohistochemical localization of NOS isoforms / Victoria Cattell and Karen Mosley -- A practical protocol for the demonstration of NOS using in-situ hybridization / G. Michael Taylor and H. Terence Cook -- Use of NO donors in biological systems / Jayne M. Tullett and Daryl D. Rees -- Making and working with peroxynitrite / Roger White [and others] -- The use of NO gas in biological systems / Neil Hogg and B. Kalyanaraman -- A microtiter-plate assay of human NOS isoforms / John Dawson and Richard G. Knowles -- Use of arginine analogs as inhibitors of nitric oxide synthase in rat-aortic rings / Rachel J. Russell -- Measurement of biopterin and the use of inhibitors of biopterin biosynthesis / Kazuyuki Hatakeyama -- A sensitive assay for the enzymatic activity of GTP cyclohydrolase I / Kazuyuki Hatakeyama and Toshie Yoneyama -- Simultaneous measurement of mitochondrial function and NO / Ignacio Lizasoain and María A. Moro -- Release of NO from donor compounds : a mathematical model for calculation of NO concentrations in the presence of oxygen / Kurt Schmidt [and others] -- The determination of nitrotyrosine residues in proteins / Andrew J. Gow [and others] -- Measurement of DNA damage using the comet assay / Steven Thomas [and others] -- Methods for the study of NO-induced apoptosis in cultured cells / Anne C. Loweth and Noel G. Morgan.

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Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.

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