In vitro transcription and translation protocols.

Contributor(s): Grandi, Guido
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 375.Publisher: Totowa, N.J. : Humana Press, ©2007Edition: 2nd ed. / edited by Guido GrandiDescription: 1 online resource (xi, 276 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1588295583; 9781588295583; 9781597453882; 1597453889; 128097186X; 9781280971860Subject(s): Molecular genetics -- Laboratory manuals | Genetic transcription -- Laboratory manuals | Genetic translation -- Laboratory manuals | Genetic Techniques | Molecular Biology | Protein Biosynthesis | Transcription, Genetic | SCIENCE -- Life Sciences -- Molecular Biology | Genetic translation | Molecular biology | Protein biosynthesis | Transcription, Genetic | Genetic Techniques | Molecular genetics | Genetic transcription | Genetic transcription | Genetic translation | Molecular geneticsGenre/Form: Laboratory Manual. | Fulltext. | Internet Resource. | Laboratory Manuals. | Electronic books. | Handbooks and manuals. Additional physical formats: Print version:: In vitro transcription and translation protocols.DDC classification: 572.8/845078 LOC classification: QH442 | .I557 2007Other classification: Q75-33 Online resources: Click here to access online
Contents:
Energy systems for ATP regeneration in cell-free protein synthesis reactions / Kara A. Calhoun and James R. Swartz -- Continuous-exchange protein-synthesizing systems / Vladimir A. Shirokov [and others] -- Cell-free production of integral membrane proteins on a preparative scale / Christian Klammt [and others] -- SIMPLEX : Single-molecule PCR-linked in vitro expression : a novel method for high-throughput construction and screening of protein libraries / Suang Rungpragayphan, Tsuneo Yamane, and Hideo Nakano -- Methods for high-throughput materialization of genetic information based on the wheat germ cell-free expression system / Tatsuya Sawasaki [and others] -- Exogenous protein expression in Xenopus oocytes : basic procedures / Elena Bossi, Maria Serena Fabbrini, and Aldo Ceriotti -- In vitro translation to study HIV protease activity / Zene Matsuda [and others].
The protein truncation test in mutation detection and molecular diagnosis / Oliver Hauss and Oliver Müller -- Creation of novel enantioselective lipases by SIMPLEX / Yuichi Koga, Tsuneo Yamane, and Hideo Nakano -- In vitro transcription and translation coupled with two-dimensional electrophoresis for bacterial proteome analysis / Nathalie Norais [and others] -- In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation, and immunoprecipitation analysis / Orit Gat, Haim Grosfeld, and Avigdor Shafferman -- Functional expression of Type 1 rat GABA transporter in microinjected Xenopus laevis oocytes / Stefano Giovannardi [and others] -- Production of protein for nuclear magnetic resonance study using the wheat germ cell-free system / Toshiyuki Kohno and Yaeta Endo.
Summary: A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.
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Includes bibliographical references and index.

Energy systems for ATP regeneration in cell-free protein synthesis reactions / Kara A. Calhoun and James R. Swartz -- Continuous-exchange protein-synthesizing systems / Vladimir A. Shirokov [and others] -- Cell-free production of integral membrane proteins on a preparative scale / Christian Klammt [and others] -- SIMPLEX : Single-molecule PCR-linked in vitro expression : a novel method for high-throughput construction and screening of protein libraries / Suang Rungpragayphan, Tsuneo Yamane, and Hideo Nakano -- Methods for high-throughput materialization of genetic information based on the wheat germ cell-free expression system / Tatsuya Sawasaki [and others] -- Exogenous protein expression in Xenopus oocytes : basic procedures / Elena Bossi, Maria Serena Fabbrini, and Aldo Ceriotti -- In vitro translation to study HIV protease activity / Zene Matsuda [and others].

The protein truncation test in mutation detection and molecular diagnosis / Oliver Hauss and Oliver Müller -- Creation of novel enantioselective lipases by SIMPLEX / Yuichi Koga, Tsuneo Yamane, and Hideo Nakano -- In vitro transcription and translation coupled with two-dimensional electrophoresis for bacterial proteome analysis / Nathalie Norais [and others] -- In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation, and immunoprecipitation analysis / Orit Gat, Haim Grosfeld, and Avigdor Shafferman -- Functional expression of Type 1 rat GABA transporter in microinjected Xenopus laevis oocytes / Stefano Giovannardi [and others] -- Production of protein for nuclear magnetic resonance study using the wheat germ cell-free system / Toshiyuki Kohno and Yaeta Endo.

Print version record.

A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.

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