Transgenic Xenopus : microinjection methods and developmental neurobiology / by Shlomo Seidman and Hermona Soreq.
By: Seidman, Shlomo
Contributor(s): Soreq, HMaterial type: TextSeries: Neuromethods: 28.Publisher: Totowa, N.J. : Humana Press, ©1997Description: 1 online resource (xviii, 198 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592596331; 1592596339Subject(s): Xenopus laevis | Frogs -- Genetics | Developmental neurobiology | Xenopus -- embryology | Animals, Genetically Modified -- embryology | Neurobiology -- methods | Developmental neurobiology | Frogs -- Genetics | Xenopus laevisGenre/Form: Electronic books. Additional physical formats: Print version: Seidman, Shlomo.: Transgenic xenopus.DDC classification: 591.3/5 LOC classification: QL668.E265 | S45 1997Online resources: Click here to access online
|Item type||Current location||Collection||Call number||Status||Date due||Barcode||Item holds|
Includes bibliographical references (pages 167-193) and index.
Print version record.
Preface to the Series -- Preface -- Acknowledgments -- Contents -- List of Color Plates -- Chapter 1 Scientific Background -- Chapter 2 Experimental Methodologies -- Chapter 3 Experimental Applications Human Acetylcholinesterase as a Model Nervous System Protein -- Chapter 4 Conclusions -- Appendices to Experimental Methodologies -- Appendix I Worldwide Xenopus Suppliers African Xenopus Facility -- Appendix II Laboratory Maintenance of Xenopus he & s Frogs Housing -- Appendix III Preparation of Buffers/Reagents for Microinjections Modified Barthâ€?s Medium
Appendix IV Microinjection EquipmentAppendix V In Vitro Fertilization and Microinjection of Xenopus Embryos -- Appendix VI Whole-Mount Staining for Catalytically Active Acetylcholinesterase -- Appendix VII Isolation of Xenopus Oocytes Using Collagenase -- References -- Index
The need to better understand the molecular, b- chemical, and cellular processes by which a developing neuronal system unfolds has led to the development of a unique set of experimental tools and organisms. Special emphasis was devoted to allowing us access, at the ear- est stages, to the genomic basis underlying the system's ultimate complexity, as exhibited once its structures are fully formed. Yet, nerve cells are anatomically, physiolo- cally, and biochemically diverse. The multitude of d- tinctly different routes for their development thus makes the developing nervous system especially intriguing for molecular neurobiologists. In particular, the demands of modern molecular neuroscience call for the establishment of efficient yet versatile systems for studying these c- plex processes. Transgenic embryos of the frog Xenopus laevis offer an excellent system for approaching neuroscientific issues. Insertion of foreign genes is performed simply, by mic- injection under binocular observation; hundreds of in vitro-fertilized embryos can be microinjected in one experiment. Embryos develop in tap water, at room t- perature, and within a few days become independent swimming tadpoles with fully functioning neuromus- lar systems. Being relatively small, these organisms are amenable to detailed analyses at the levels of mRNA, protein, and cell. Their rapid development permits the study of morphogenetic processes involved in early development, such as myogenesis and neural induction, as well as those involved in organogenesis and formation of the brain, the musculature, and the interconnections between them. Foreign DNA remains predominantly extrachromosomal.