Apoptosis techniques and protocols.

Contributor(s): LeBlanc, Andréa C
Material type: TextTextSeries: Neuromethods: 37.Publisher: Totowa, N.J. : Humana, ©2002Edition: 2nd ed. / edited by Andréa C. LeblancDescription: 1 online resource (xv, 250 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1588290123; 9781588290120; 9781592591886; 1592591884Subject(s): Apoptosis -- Laboratory manuals | Apoptosis -- physiology | Brain Diseases -- physiopathology | Histocytological Preparation Techniques | Neurons -- physiology | SCIENCE -- Life Sciences -- Anatomy & Physiology | ApoptosisGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Apoptosis techniques and protocols.DDC classification: 571.9/36 LOC classification: QH671 | .A6595 2002Online resources: Click here to access online
Contents:
Molecular characterization of the proapoptotic protein bax / Yi-Te Hsu and Soraya Smaili -- Assessing cytochrome-c release from mitochondria / Douglas W. Ethell and Douglas R. Green -- Monitoring caspases in neuronal cell death / Younes Bounhar, Omar Tounekti, and Andréa C. LeBlanc -- HSV amplicon vectors in neuronal apoptosis studies / Kathleen A. Maguire-Zeiss, William J. Bowers, and Howard J. Federoff -- Microinjections to study the specific role of proapoptotic proteins in neurons / Yan Zhang and Andréa C. LeBlanc -- Designing microarray experiments for neurobiology / P. Scott Eastman and Jeanne F. Loring -- Analysis of gene expression by nylon membrane cDNA arrays / H. Michael Tucker and Steven Estus -- Glyceraldehyde-3-phosphate dehydrogenase as a target for antiapoptotic drugs / Mark D. Berry and Paula C. Ashe -- Analytical approaches for investigating apoptosis and other biochemical events in compartmented cultures of sympathetic neurons / Barbara Karten [and others] -- Detection and analysis of synaptosis / Greg M. Cole and Karen Gylys -- In situ detection of apoptotic neurons / Kevin A. Roth -- Apoptotic and oxidative indicators in Alzheimer's Disease / Arun K. Raina [and others].
Summary: The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature. Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death.
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Includes bibliographical references and index.

Molecular characterization of the proapoptotic protein bax / Yi-Te Hsu and Soraya Smaili -- Assessing cytochrome-c release from mitochondria / Douglas W. Ethell and Douglas R. Green -- Monitoring caspases in neuronal cell death / Younes Bounhar, Omar Tounekti, and Andréa C. LeBlanc -- HSV amplicon vectors in neuronal apoptosis studies / Kathleen A. Maguire-Zeiss, William J. Bowers, and Howard J. Federoff -- Microinjections to study the specific role of proapoptotic proteins in neurons / Yan Zhang and Andréa C. LeBlanc -- Designing microarray experiments for neurobiology / P. Scott Eastman and Jeanne F. Loring -- Analysis of gene expression by nylon membrane cDNA arrays / H. Michael Tucker and Steven Estus -- Glyceraldehyde-3-phosphate dehydrogenase as a target for antiapoptotic drugs / Mark D. Berry and Paula C. Ashe -- Analytical approaches for investigating apoptosis and other biochemical events in compartmented cultures of sympathetic neurons / Barbara Karten [and others] -- Detection and analysis of synaptosis / Greg M. Cole and Karen Gylys -- In situ detection of apoptotic neurons / Kevin A. Roth -- Apoptotic and oxidative indicators in Alzheimer's Disease / Arun K. Raina [and others].

Print version record.

The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature. Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death.

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