Regulatory protein modification : techniques and protocols / edited by Hugh C. Hemmings, Jr.Material type: TextSeries: Neuromethods ; 30.Publication details: Totowa, N.J. : Humana Press, ©1997. Description: 1 online resource (xiv, 445 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592596355; 1592596355Subject(s): Proteins -- Chemical modification -- Handbooks, manuals, etc | Proteins -- Analysis -- Handbooks, manuals, etc | Neurosciences -- Handbooks, manuals, etc | Protein kinases -- Handbooks, manuals, etc | Phosphorylation | Protein Processing, Post-Translational -- physiology | Neurons -- physiology | Protein Engineering -- methods | Protein Kinases | Synaptosomes -- chemistry | Neurosciences | Protein kinases | Proteins -- Analysis | Proteins -- Chemical modification | Nervenzelle | Proteine | Posttranslationale Änderung | AufsatzsammlungGenre/Form: Laboratory Manual. | Electronic books. | Handbooks and manuals. Additional physical formats: Print version:: Regulatory protein modification.DDC classification: 612.8 LOC classification: QP551 | .R45 1997NLM classification: W1 | QH 450.6Online resources: Click here to access online
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Includes bibliographical references and index.
Analysis of Protein Phosphorylation in Intact Cells and Extracts / S. Ivar Walaas and Anne Carine Ostvold -- Analysis of Protein Dephosphorylation in Intact Cells and Extracts / Shelley Halpain -- Protein Phosphorylation and Dephosphorylation in Isolated Nerve Terminals (Synaptosomes) / Talvinder S. Sirha -- Protein Kinase and Phosphotase Inhibitors: Applications in Neuroscience / Hugh C. Hemmings, Jr. -- Phosphorylation State-Specific Antibodies / Andres J. Czernik, Jeffrey Mathers and Sheenah M. Mische -- Protein Tyrosine Phosphorylation / Pascal Derkinderen and Jean-Antoine Girault -- Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis / James A. Bibb and Edgar F. da Cruz e Silva -- Protein Methylation in the Nervous System / Darin J. Weber and Philip N. McFadden -- Long-Chain Fatty Acylation of Proteins / Sean I. Patterson and J.H. Pate Skene -- Protein ADP-Ribosylation / Keith D. Philibert and Henk Zwiers.
Print version record.
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Hugh Hemmings' Regulatory Protein Modification: Techniques and Protocols offers critical reviews of major topics concerning posttranslational protein modification in neurons, as well as a comprehensive collection of state-of-the-art techniques for their analysis. Its distinguished international contributors provide detailed and specific methods for studying protein phosphorylation/dephospohorylation, methylation, long chain fatty acylation, ADP-ribosylation, glycosylation, and glycosylphosphatidylinositol anchors. Using both conventional analytical and novel molecular biological approaches, these experts discuss the techniques and reagents and cite numerous examples from the neuroscience literature in a way that allows investigators to determine the best experimental approaches for their own systems. They also offer a complete review of protein kinase and phosphatase inhibitors techniques and their applications today. Regulatory Protein Modification: Techniques and Protocols will prove an exceptionally valuable resource for both experienced and new investigators working in neuronal and nonneuronal systems.