Regulatory protein modification : techniques and protocols / edited by Hugh C. Hemmings, Jr.

Contributor(s): Hemmings, Hugh C., Jr Material type: Text

TextSeries: Neuromethods ; 30.Publication details: Totowa, N.J. : Humana Press, ©1997. Description: 1 online resource (xiv, 445 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592596355; 1592596355Subject(s): Proteins -- Chemical modification -- Handbooks, manuals, etc | Proteins -- Analysis -- Handbooks, manuals, etc | Neurosciences -- Handbooks, manuals, etc | Protein kinases -- Handbooks, manuals, etc | Phosphorylation | Protein Processing, Post-Translational -- physiology | Neurons -- physiology | Protein Engineering -- methods | Protein Kinases | Synaptosomes -- chemistry | Neurosciences | Protein kinases | Proteins -- Analysis | Proteins -- Chemical modification | Nervenzelle | Proteine | Posttranslationale Änderung | AufsatzsammlungGenre/Form: Laboratory Manual. | Electronic books. | Handbooks and manuals. Additional physical formats: Print version:: Regulatory protein modification.DDC classification: 612.8 LOC classification: QP551 | .R45 1997NLM classification: W1 | QH 450.6Online resources: Click here to access online

Contents:

Analysis of Protein Phosphorylation in Intact Cells and Extracts / S. Ivar Walaas and Anne Carine Ostvold -- Analysis of Protein Dephosphorylation in Intact Cells and Extracts / Shelley Halpain -- Protein Phosphorylation and Dephosphorylation in Isolated Nerve Terminals (Synaptosomes) / Talvinder S. Sirha -- Protein Kinase and Phosphotase Inhibitors: Applications in Neuroscience / Hugh C. Hemmings, Jr. -- Phosphorylation State-Specific Antibodies / Andres J. Czernik, Jeffrey Mathers and Sheenah M. Mische -- Protein Tyrosine Phosphorylation / Pascal Derkinderen and Jean-Antoine Girault -- Identification of Posttranslational Modification Sites by Site-Directed Mutagenesis / James A. Bibb and Edgar F. da Cruz e Silva -- Protein Methylation in the Nervous System / Darin J. Weber and Philip N. McFadden -- Long-Chain Fatty Acylation of Proteins / Sean I. Patterson and J.H. Pate Skene -- Protein ADP-Ribosylation / Keith D. Philibert and Henk Zwiers.

Action note: digitized 2010 committed to preserveSummary: Hugh Hemmings' Regulatory Protein Modification: Techniques and Protocols offers critical reviews of major topics concerning posttranslational protein modification in neurons, as well as a comprehensive collection of state-of-the-art techniques for their analysis. Its distinguished international contributors provide detailed and specific methods for studying protein phosphorylation/dephospohorylation, methylation, long chain fatty acylation, ADP-ribosylation, glycosylation, and glycosylphosphatidylinositol anchors. Using both conventional analytical and novel molecular biological approaches, these experts discuss the techniques and reagents and cite numerous examples from the neuroscience literature in a way that allows investigators to determine the best experimental approaches for their own systems. They also offer a complete review of protein kinase and phosphatase inhibitors techniques and their applications today. Regulatory Protein Modification: Techniques and Protocols will prove an exceptionally valuable resource for both experienced and new investigators working in neuronal and nonneuronal systems.

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Includes bibliographical references and index.

Print version record.

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Electronic reproduction. [Place of publication not identified] : HathiTrust Digital Library, 2010. MiAaHDL

Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL

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Hugh Hemmings' Regulatory Protein Modification: Techniques and Protocols offers critical reviews of major topics concerning posttranslational protein modification in neurons, as well as a comprehensive collection of state-of-the-art techniques for their analysis. Its distinguished international contributors provide detailed and specific methods for studying protein phosphorylation/dephospohorylation, methylation, long chain fatty acylation, ADP-ribosylation, glycosylation, and glycosylphosphatidylinositol anchors. Using both conventional analytical and novel molecular biological approaches, these experts discuss the techniques and reagents and cite numerous examples from the neuroscience literature in a way that allows investigators to determine the best experimental approaches for their own systems. They also offer a complete review of protein kinase and phosphatase inhibitors techniques and their applications today. Regulatory Protein Modification: Techniques and Protocols will prove an exceptionally valuable resource for both experienced and new investigators working in neuronal and nonneuronal systems.

Other editions of this work

Other editions
	Apoptosis techniques and protocols / ©1997
	Apoptosis techniques and protocols / ©1997