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Mitochondria : practical protocols / edited by Dario Leister, Johannes M. Herrmann.

Contributor(s): Leister, Dario | Herrmann, Johannes (Johannes M.)Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.) ; v. 372.Publication details: Totowa, N.J. : Humana Press, ©2007. Description: 1 online resource (xvi, 592 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1588296679; 9781588296672; 159745365X; 9781597453653Subject(s): Mitochondria -- Laboratory manuals | Mitochondria -- physiology | Mitochondria -- genetics | Mitochondrial Proteins | Models, Biological | Mitochondries -- Manuels de laboratoire | SCIENCE -- Life Sciences -- Cell Biology | Mitochondria -- physiology | Mitochondria -- genetics | Mitochondrial Proteins | Models, Biological | Mitochondria | MitochondriaGenre/Form: Laboratory Manual | Fulltext. | Internet Resource. | Laboratory Manuals. | Laboratory manuals. Additional physical formats: Print version:: Mitochondria.DDC classification: 571.6/57 LOC classification: QH603.M5 | M58 2007NLM classification: QU 25 | W1Other classification: Q244 Online resources: Click here to access online
Contents:
The mitochondria of cultured mammalian cells: I. Analysis by immunofluorescence microscopy, histochemistry, subcellular fractionation, and cell fusion / F. Malka [and others] -- The mitochondria of cultured mammalian cells: Ii. Expression and visualization of exogenous proteins in fixed and live cells / S. Goffart [and others] -- Drosophila melanogaster as a model system to study mitochondrial biology / M.A. FeRNAndez-Moreno [and others] -- Isolation and functional analysis of mitochondria from the nematode caenorhabditis elegans / L.I. Grad, L.C. Sayles and B.D. Lemire -- Isolation of mitochondria from procyclic Trypanosoma brucei / A. Schneider [and others] -- Saccharomyces cerevisiae as a model organism to study mitochondrial biology: General considerations and basic procedures / K. Altmann, M. Durr and B. Westermann -- Studying mitochondria in an attractive model: Schizosaccharomyces pombe / S. Chiron [and others] -- Neurospora crassa as a model organism for mitochondrial biogenesis / F.E. Nargang and D. Rapaport -- Isolation of intact, functional mitochondria from the model plant Arabidopsis thaliana / L.J. Sweetlove, N.L. Taylor and C.J. Leaver -- Chlamydomonas reinhardtii: The model of choice to study mitochondria from unicellular photosynthetic organisms / S. Funes, L.G. Franzen and D. Gonzalez-Halphen -- Directed alteration of Saccharomyces cerevisiae mitochondrial DNA by biolistic transformation and homologous recombination / N. Bonnefoy and T.D. Fox -- Screens for mitochondrial mutants in yeast / F. Foury -- Transcript end mapping and analysis of RNA editing in plant mitochondria / F. Kempken [and others] -- In vitro analysis of the yeast mitochondrial RNA polymerase / E.A. Amiott and J.A. Jaehning -- Functional analysis by inducible RNA interference in Drosophila melanogaster / Y. Matsushima [and others] -- Analysis of replicating mitochondrial DNA by two-dimensional agarose gel electrophoresis / A. Reyes, T. Yasukawa and I.J. Holt -- The analysis of tRNA import into mammalian mitochondria / A.M. Mager-Heckel [and others] -- Analysis of mitochondrial protein synthesis in yeast / S. Funes and J.M. Herrmann -- Large-scale isolation of mitochondrial ribosomes from mammalian tissues / L.L. Spremulli -- Protein import into isolated mitochondria / D. Mokranjac and W. Neupert -- Analyzing import intermediates of mitochondrial proteins by blue native gel electrophoresis / T. Waizenegger and D. Rapaport -- In vitro and in vivo protein import into plant mitochondria / P.F. Pavlov [and others] -- Two-dimensional blue native/blue native polyacrylamide gel electrophoresis for the characterization of mitochondrial protein complexes and supercomplexes / S. Sunderhaus, H. Eubel and H.P. Braun -- Investigation of iron-sulfur protein maturation in eukaryotes / O. Stehling [and others] -- Studying proteolysis within mitochondria / T. Tatsuta and T. Langer -- Methods to determine the status of mitochondrial ATP synthase assembly / S.H. Ackerman and A. Tzagoloff -- Atp production in isolated mitochondria of procyclic trypanosoma brucei / A. Schneider [and others] -- Oxidative stress and plant mitochondria / N.L. Taylor and A.H. Millar -- Measuring mitochondrial shape changes and their consequences on mitochondrial involvement during apoptosis / C. Frezza, S. Cipolat and L. Scorrano -- Assessing mitochondrial potential, calcium, and redox state in isolated mammalian cells using confocal microscopy / S.M. Davidson, D. Yellon and M.R. Duchen -- Fluorescence imaging of mitochondria in yeast / T.C. Swayne, A.C. Gay and L.A. Pon -- Mitochondrial fusion in vitro / S.L. Meeusen and J. Nunnari -- Conventional and immunoelectron microscopy of mitochondria / E.M. Perkins and J.M. McCaffery -- Mitochondrial division in Caenorhabditis elegans / S. Gandre and A.M. van der Bliek -- Yeast mitochondrial transcriptomics / M. Garcia [and others] -- Plant mitochondrial transcriptomics by quantitative RT-PCR / R. Clifton and J. Whelan -- Proteomics of yeast mitochondria / J. Reinders and A. Sickmann -- Arabidopsis mitochondrial proteomics / J.L. Heazlewood and A.H. Millar -- MitoP2, an integrated database for mitochondrial proteins / H. Prokisch and U. Ahting.
Summary: Mitochondria: Practical Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.
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Includes bibliographical references and index.

The mitochondria of cultured mammalian cells: I. Analysis by immunofluorescence microscopy, histochemistry, subcellular fractionation, and cell fusion / F. Malka [and others] -- The mitochondria of cultured mammalian cells: Ii. Expression and visualization of exogenous proteins in fixed and live cells / S. Goffart [and others] -- Drosophila melanogaster as a model system to study mitochondrial biology / M.A. FeRNAndez-Moreno [and others] -- Isolation and functional analysis of mitochondria from the nematode caenorhabditis elegans / L.I. Grad, L.C. Sayles and B.D. Lemire -- Isolation of mitochondria from procyclic Trypanosoma brucei / A. Schneider [and others] -- Saccharomyces cerevisiae as a model organism to study mitochondrial biology: General considerations and basic procedures / K. Altmann, M. Durr and B. Westermann -- Studying mitochondria in an attractive model: Schizosaccharomyces pombe / S. Chiron [and others] -- Neurospora crassa as a model organism for mitochondrial biogenesis / F.E. Nargang and D. Rapaport -- Isolation of intact, functional mitochondria from the model plant Arabidopsis thaliana / L.J. Sweetlove, N.L. Taylor and C.J. Leaver -- Chlamydomonas reinhardtii: The model of choice to study mitochondria from unicellular photosynthetic organisms / S. Funes, L.G. Franzen and D. Gonzalez-Halphen -- Directed alteration of Saccharomyces cerevisiae mitochondrial DNA by biolistic transformation and homologous recombination / N. Bonnefoy and T.D. Fox -- Screens for mitochondrial mutants in yeast / F. Foury -- Transcript end mapping and analysis of RNA editing in plant mitochondria / F. Kempken [and others] -- In vitro analysis of the yeast mitochondrial RNA polymerase / E.A. Amiott and J.A. Jaehning -- Functional analysis by inducible RNA interference in Drosophila melanogaster / Y. Matsushima [and others] -- Analysis of replicating mitochondrial DNA by two-dimensional agarose gel electrophoresis / A. Reyes, T. Yasukawa and I.J. Holt -- The analysis of tRNA import into mammalian mitochondria / A.M. Mager-Heckel [and others] -- Analysis of mitochondrial protein synthesis in yeast / S. Funes and J.M. Herrmann -- Large-scale isolation of mitochondrial ribosomes from mammalian tissues / L.L. Spremulli -- Protein import into isolated mitochondria / D. Mokranjac and W. Neupert -- Analyzing import intermediates of mitochondrial proteins by blue native gel electrophoresis / T. Waizenegger and D. Rapaport -- In vitro and in vivo protein import into plant mitochondria / P.F. Pavlov [and others] -- Two-dimensional blue native/blue native polyacrylamide gel electrophoresis for the characterization of mitochondrial protein complexes and supercomplexes / S. Sunderhaus, H. Eubel and H.P. Braun -- Investigation of iron-sulfur protein maturation in eukaryotes / O. Stehling [and others] -- Studying proteolysis within mitochondria / T. Tatsuta and T. Langer -- Methods to determine the status of mitochondrial ATP synthase assembly / S.H. Ackerman and A. Tzagoloff -- Atp production in isolated mitochondria of procyclic trypanosoma brucei / A. Schneider [and others] -- Oxidative stress and plant mitochondria / N.L. Taylor and A.H. Millar -- Measuring mitochondrial shape changes and their consequences on mitochondrial involvement during apoptosis / C. Frezza, S. Cipolat and L. Scorrano -- Assessing mitochondrial potential, calcium, and redox state in isolated mammalian cells using confocal microscopy / S.M. Davidson, D. Yellon and M.R. Duchen -- Fluorescence imaging of mitochondria in yeast / T.C. Swayne, A.C. Gay and L.A. Pon -- Mitochondrial fusion in vitro / S.L. Meeusen and J. Nunnari -- Conventional and immunoelectron microscopy of mitochondria / E.M. Perkins and J.M. McCaffery -- Mitochondrial division in Caenorhabditis elegans / S. Gandre and A.M. van der Bliek -- Yeast mitochondrial transcriptomics / M. Garcia [and others] -- Plant mitochondrial transcriptomics by quantitative RT-PCR / R. Clifton and J. Whelan -- Proteomics of yeast mitochondria / J. Reinders and A. Sickmann -- Arabidopsis mitochondrial proteomics / J.L. Heazlewood and A.H. Millar -- MitoP2, an integrated database for mitochondrial proteins / H. Prokisch and U. Ahting.

Print version record.

Mitochondria: Practical Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.

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