Baculovirus and insect cell expression protocols / edited by David W. Murhammer.

Contributor(s): Murhammer, David W
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 388.Publisher: Totowa, N.J. : Humana Press, ©2007Edition: 2nd edDescription: 1 online resource (xiv, 470 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 1588295370; 9781588295378; 1597454575; 9781597454575Uniform titles: Baculovirus expression protocols. Subject(s): Baculoviruses -- Genetics | Genetic engineering | Virus-vector relationships | Insect cell biotechnology | Baculoviridae -- genetics | Gene Expression | Genetic Techniques | Genetic Vectors | SCIENCE -- Life Sciences -- Biology | SCIENCE -- Life Sciences -- Microbiology | Insect cell biotechnology | Baculoviridae -- genetics | Gene expression | Genetic Techniques | Genetic vectors | Baculoviruses -- Genetics | Genetic engineering | Virus-vector relationships | Baculoviruses -- Genetics | Genetic engineering | Insect cell biotechnology | Virus-vector relationships | baculovirus | baculoviridae | insectenvirussen | insect viruses | genetica | genetics | genetische modificatie | genetic engineering | genetische vectoren | genetic vectors | genexpressie | gene expression | insecten | insects | cellen | cells | recombinant eiwitten | recombinant proteins | moleculaire biologie | molecular biology | biologische technieken | biological techniques | laboratoriummethoden | laboratory methods | bacterial insecticides | protocollen | protocols | Molecular Biology (General) | Genetic Engineering | Moleculaire biologie (algemeen) | Genetische modificatieGenre/Form: Electronic books. Additional physical formats: Print version:: Baculovirus and insect cell expression protocols.DDC classification: 579.2/436 LOC classification: QR398.5 | .B325 2007Other classification: S432. 4 Online resources: Click here to access online
Contents:
Useful tips, widely used techniques, and quantifying cell metabolic behavior / D.W. Murhammer -- Introduction to baculovirus molecular biology / B.J. Kelly [and others] -- Baculovirus transfer vectors / R.D. Possee [and others] -- Recombinant baculovirus isolation / L.A. King [and others] -- Gene expression in mammalian cells using BacMam, a modified baculovirus system / J.A. Fornwald [and others] -- Available lepidopteran insect cell lines / D.E. Lynn -- Lepidopteran insect cell line isolation from insect tissue / D.E. Lynn -- Development of serum-free media for lepidopteran insect cell lines / S.N. Agathos -- Routine maintenance and storage of lepidopteran insect cell lines and baculoviruses / D.E. Lynn -- Small-scale protein production with the baculovirus expression vector system / S. Harwood -- Recombinant protein production in large-scale agitated bioreactors using the baculovirus expression vector system / C.B. Elias [and others] -- Production of baculovirus-expressed recombinant proteins in wave bioreactors / S.H. Kadwell [and others] -- Protein production with recombinant baculoviruses in lepidopteran larvae / Y. Liu [and others] -- Virus-like particle and viral vector production using the baculovirus expression vector system/insect cell system: Adeno-associated virus-based products / M.G. Aucoin [and others] -- Transforming lepidopteran insect cells for continuous recombinant protein expression / R.L. Harrison [and others] -- Transformation of drosophila cell lines: An alternative approach to exogenous protein expression / L. Cherbas [and others] -- Transforming lepidopteran insect cells for improved protein processing / R.L. Harrison [and others] -- Introduction to the use of baculoviruses as biological insecticides / B.C. Bonning [and others] -- Baculovirus insecticide production in insect larvae / N. van Beek [and others] -- Evaluation of the insecticidal efficacy of wild-type and recombinant baculoviruses / H. Li [and others] -- Monitoring and visualization of baculovirus infection using green fluorescent protein strategy / H.J. Cha [and others] -- Alternative bioreactor strategy for probing infection and production / Y.-C. Hu [and others] -- Methods for gene silencing with rnai / J.C. March [and others] -- Use of the baculovirus/insect cell system to study apoptosis / N. Chejanovsky -- Generation of envelope-modified baculoviruses for gene delivery into mammalian cells / C. Hofmann.
In: Springer ProtocolsSummary: Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.
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Revised edition of: Baculovirus expression protocols. c1995.

Includes bibliographical references and index.

Title from title screen (viewed Nov. 20, 2007).

Useful tips, widely used techniques, and quantifying cell metabolic behavior / D.W. Murhammer -- Introduction to baculovirus molecular biology / B.J. Kelly [and others] -- Baculovirus transfer vectors / R.D. Possee [and others] -- Recombinant baculovirus isolation / L.A. King [and others] -- Gene expression in mammalian cells using BacMam, a modified baculovirus system / J.A. Fornwald [and others] -- Available lepidopteran insect cell lines / D.E. Lynn -- Lepidopteran insect cell line isolation from insect tissue / D.E. Lynn -- Development of serum-free media for lepidopteran insect cell lines / S.N. Agathos -- Routine maintenance and storage of lepidopteran insect cell lines and baculoviruses / D.E. Lynn -- Small-scale protein production with the baculovirus expression vector system / S. Harwood -- Recombinant protein production in large-scale agitated bioreactors using the baculovirus expression vector system / C.B. Elias [and others] -- Production of baculovirus-expressed recombinant proteins in wave bioreactors / S.H. Kadwell [and others] -- Protein production with recombinant baculoviruses in lepidopteran larvae / Y. Liu [and others] -- Virus-like particle and viral vector production using the baculovirus expression vector system/insect cell system: Adeno-associated virus-based products / M.G. Aucoin [and others] -- Transforming lepidopteran insect cells for continuous recombinant protein expression / R.L. Harrison [and others] -- Transformation of drosophila cell lines: An alternative approach to exogenous protein expression / L. Cherbas [and others] -- Transforming lepidopteran insect cells for improved protein processing / R.L. Harrison [and others] -- Introduction to the use of baculoviruses as biological insecticides / B.C. Bonning [and others] -- Baculovirus insecticide production in insect larvae / N. van Beek [and others] -- Evaluation of the insecticidal efficacy of wild-type and recombinant baculoviruses / H. Li [and others] -- Monitoring and visualization of baculovirus infection using green fluorescent protein strategy / H.J. Cha [and others] -- Alternative bioreactor strategy for probing infection and production / Y.-C. Hu [and others] -- Methods for gene silencing with rnai / J.C. March [and others] -- Use of the baculovirus/insect cell system to study apoptosis / N. Chejanovsky -- Generation of envelope-modified baculoviruses for gene delivery into mammalian cells / C. Hofmann.

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

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