Protein targeting protocols / edited by Mark van der Giezen.

Includes bibliographical references and index.

Localization and mobility of bacterial proteins by confocal microscopy and fluorescene recovery after photobleaching / Conrad W. Mullineaux -- Membrane protein insertion and secretion in bacteria / Jeanine de Keyzer, Martin van der Laan, Arnold J.M. Driessen -- Identification of autotransporter proteins secreted by type V secretion systems in gram-negative bacteria / Yihfen T. Yen, Christos Stathopoulos -- Periplasmic targeting of recombinant proteins in Escherichia coli / Filipe J. Mergulhãu, Gabriel A. Monteiro -- In Vitro analysis of the bacterial twin-arginine-dependent protein export / Michael Moser, Sascha Panahandeh, Eva Holzapfel, Matthias Muller -- Nuclear import properties of the sex-determining factor SRY / Jade K. Forwood, Gurpreet Kaur, David A. Jans -- The mitochondrial machinery for import of precursor proteins / Kipros Gabriel, Nikolaus Pfanner -- A genetic method to identify mitochondrial proteins in living mammalian cells / Takeaki Ozawa, Yoshio Umezawa -- In vitro and in vivo methods to study protein import into plant mitochondria / Shashi Bhushan [and others] -- Protein targeting to mitochondria of saccharomyces cerevisiae and neurospora crassa: in vitro and in vivo studies / Panagiotis Papatheodorou, Grażyna Domańska, Joachim Rassow -- A mitosome purification protocol based on percoll density gradients and its use in validating the mitosomal nature of entamoeba histolytica mitochondrial Hsp70 / Jorge Tovar, Siân S.E. Cox, Mark ven der Giezen -- The chloroplast protein import machinery: a review / Friederike Hörmann, Jürgen Soll, Bettina Bölter -- Import of plastid precursor proteins into pea chloroplasts / Bettina Bölter, Jürgen Soll -- Protein targeting in "secondary" or "complex" chloroplasts / Balbir K. Chaal, Beverly R. Green -- Protein trafficking to the complex chloroplasts of Euglena / Rostislav Vacula, Silvia Sláviková, Stepahn D. Schwartzbach -- Fluorescent protein fusions for protein localization in plants / John Runions, Chris Hawes, SMita Kurup -- Genetic transformation: a tool to study protein targeting in diatoms / Peter G. Kroth -- Identification of proteins targeted into the endoplasmic reticulum by cDNA library screening / Takeaki Ozawa, Yoshio Umezama -- Trafficking through the early secretory pathway of mammalian cells / Theresa H. Ward -- Studying protein export from the endoplasmic reticulum in plants / Sally L. Hanton, Loren A. Matheson, Federica Brandizzi -- Imaging the golgi apparatus in living mitotic cells / Nihal Altan-Bonnet -- Evaluating yeast biosynthetic vacuolar transport / Brian A. Davies, Darren S. Carney, Bruce F. Horazdovsky -- Targeting to lysosomes in mammalian cells: the biosynthetic and endocytic pathways / Ann H. Erickson, Jeffery P. Bocock -- Monitoring autophagy in yeast: the Pho8[delta]60 assay / Daniel J. Klionsky -- Protein targeting to yeast peroxisomes / Ida van der Klei, Marten Veenhuis -- Expression, localization, and topology of fluorescently tagged plasma membrane-targeted transmembrane proteins / Jeanne Shepshelovich, Koret Hirschberg -- Intracellualr protein localization by immunoelectron microscopy / Tetsuaki Osafune, Steven D. Schwartzbach -- Seperation of proteins by blue native electrophoresis / Olga Randelj, Joachim Rassow, Christian Motz -- Computational prediction of subcelluar localization / Kenta Nakai, Paul Horton -- Phylogenetic analysis to uncover organellar origins of nuclear-encoded genes/ Bernardo J. Foth.

Print version record.

In this highly anticipated update of the extremely successful Protein Targeting Protocols, experts from around the world provide the latest protocols on for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Unlike the first edition, however, this volume places emphasis on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems. Authors provide state-of-the-art information on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Bacterial protein targeting protocols using the Sec-system, type-V secretion apparatus, and the Tat-pathway are described, as is a periplasmic targeting protocol. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available. Editor Mark van der Giezen has succeeded in including targeting protocols from different systems, including animal, plant, fungal, and protist models, providing insights into the intricate challenges presented by these diverse systems. Authoritative and state-of-the-art, Protein Targeting Protocols, Second Edition, provides useful techniques that will aid scientists working with a variety of organisms.

English.