Pyrosequencing protocols / edited by Sharon Marsh ; foreword by Howard L. McLeod.
Contributor(s): Marsh, SharonMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 373.Publisher: Totowa, N.J. : Humana Press, ©2007Description: 1 online resource (xii, 196 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1588296458; 9781588296450; 1597453773; 9781597453776Subject(s): DNA -- Analysis | Nucleotide sequence | Sequence Analysis, DNA -- methods | Methods | Sequence Analysis, DNA | Investigative Techniques | Sequence Analysis | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Genetic Techniques | MEDICAL -- Genetics | Sequence Analysis, DNA -- methods | DNA -- Analysis | Nucleotide sequence | DNA -- Analysis | Nucleotide sequenceGenre/Form: Electronic books. | Methods (Music) Additional physical formats: Print version:: Pyrosequencing protocols.DDC classification: 611/.018166 LOC classification: QP624 | .P97 2007Other classification: Q523 Online resources: Click here to access online
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Includes bibliographical references and index.
The history of pyrosequencing / Pål Nyrén -- Pyrosequencing applications / Sharon Marsh -- Web-based primer design software for genome-scale genotyping by pyrosequencing / Steven Ringquist [and others] -- Pyrosequencing : a simple method for accurate genotyping / Cristi R. King and Tiffany Scott-Horton -- Universal primer applications for pyrosequencing / Dong-Chuan Guo and Dianna M. Milewicz -- Allele quantification and DNA pooling methods / Jonathon Wasson -- Multiplex pyrosequencing for DNA variation analysis / Pritesh Patel, Yih-Horng Shiao, and Paolo Fortina -- Analysis of gene-specific DNA methylation patterns by pyrosequencing technology / Jörg Tost and Ivo Glynne Gut -- Detecting mutations that confer oxazolidinone resistance in gram-positive bacteria / Neil Woodford, Sarah E. North, and Matthew J. Ellington -- Pyrosequencing-based strategies for improved allele typing of human leukocyte antigen loci / Steven Ringquist [and others] -- Pyrosequencing of phage display libraries for the identification of cell-specific targeting ligands / Ahad A. Rahim -- Gene copy number detection in animal studies / Gerli Pielberg and Leif Andersson -- Detection of allelic imbalance in gene expression using pyrosequencing / Hua Wang and Steven C. Elbein -- Pyrosequencing genotype storage techniques / Derek J. Van Booven.
Print version record.
Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.