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Animal cell biotechnology : methods and protocols / edited by Ralf Pörtner.

Contributor(s): Pörtner, RalfMaterial type: TextTextSeries: Methods in biotechnology ; 24.Publication details: Totowa, N.J. : Humana Press, ©2007. Edition: 2nd edDescription: 1 online resource (xv, 512 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781592594863; 1592594867; 9781597453998; 1597453994; 128097155X; 9781280971556; 9786610971558; 6610971552; 9781627039635; 1627039635Subject(s): Animal cell biotechnology | Cell culture | Biotechnology -- methods | Cell Culture Techniques -- veterinary | SCIENCE -- Biotechnology | Animal cell biotechnology | Cell culture | Animal cell biotechnology | Cell culture | cellijnen | cell lines | dierlijke weefsels | animal tissues | biotechnologie | biotechnology | celkweek | cell culture | laboratoriummethoden | laboratory methods | technieken | techniques | farmaceutische producten | pharmaceutical products | celbiologie | cellular biology | protocollen | protocols | Biotechnology (General) | Cellular Biology | Biotechnologie (algemeen) | CelbiologieGenre/Form: Electronic books. Additional physical formats: Print version:: Animal cell biotechnology.DDC classification: 660.6 LOC classification: TP248.27.A53 | A542 2007ebNLM classification: 2007 F-350 | TP 248.27.A53Other classification: Q952 Online resources: Click here to access online | Click here to access online
Contents:
pt. 1. Basic techniques for establishing production cell lines -- Transduction technologies / D.A. Fluri and M. Fussenegger -- Cells for gene therapy and vector production / C. Delenda [and others] -- Technology and production of murine monoclonal and recombinant antibodies and antibody fragments / A. Dorn-Beineke, S. Nittka and M. Neumaier -- DNA fingerprinting and characterization of animal cell lines / G.N. Stacey, E. Byrne and J.R. Hawkins -- pt. 2. Basic cultivation techniques -- Microcarrier cell culture technology / G. Blüml -- Cell encapsulation / H. Hübner -- Tools for high-throughput medium and process optimization / M. Jordan and N. Jenkins -- pt. 3. Cell characterization and analysis -- Cell counting and viability measurements / M. Butler and M. Spearman -- Monitoring of growth, physiology and productivity of animal cells by flow cytometry / S. Carroll, M. Naeiri and M. Al-Rubeai -- Nuclear magnetic resonance methods for monitoring cell growth and metabolism in intensive bioreactors / A.A. Neves and K.M. Brindle -- Methods for off-line analysis of nutrients and products in mammalian cell culture / H. Büntemeyer -- Application of stoichiometric and kinetic analyses to characterize cell growth and product formation / D. Adams, R. Korke, and W.-S. Hu -- Measurement of apoptosis in cell culture / A. Ishaque1 and M. Al-Rubeai -- Metabolic flux estimation in mammalian cell cultures / C.T. Goudar [and others] -- pt. 4. Special cultivation techniques -- Disposable bioreactors for inoculum production and protein expression / R. Eibl and D. Eibl -- Hollow fiber cell culture / J.M. Davis -- Cultivation of mammalian cells in fixed bed reactors / ̦R. Pörtner and O.B.J. Platas Barradas -- Configuration of bioreactors / D.E. Martens and E.J. van den End -- pt. 5. Downstream techniques -- Membrane filtration in animal cell culture / P. Czermak, D. Nehring and R. Wickramasinghe -- Chromatographic techniques in the downstream processing of (recombinant) proteins / R. Freitag -- pt. 6. Special applications -- Vaccine production : State of the art and future needs in upstream processing / Y. Genzel and U. Reichl -- Retrovirus production and characterization / P.E. Cruz [and others] -- Insect cell cultivation and generation of recombinant baculovirus particles for recombinant protein production / S. Geisse.
Buchausg. u.d.T.:Animal cell biotechnologySummary: Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors. Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
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Includes bibliographical references and index.

pt. 1. Basic techniques for establishing production cell lines -- Transduction technologies / D.A. Fluri and M. Fussenegger -- Cells for gene therapy and vector production / C. Delenda [and others] -- Technology and production of murine monoclonal and recombinant antibodies and antibody fragments / A. Dorn-Beineke, S. Nittka and M. Neumaier -- DNA fingerprinting and characterization of animal cell lines / G.N. Stacey, E. Byrne and J.R. Hawkins -- pt. 2. Basic cultivation techniques -- Microcarrier cell culture technology / G. Blüml -- Cell encapsulation / H. Hübner -- Tools for high-throughput medium and process optimization / M. Jordan and N. Jenkins -- pt. 3. Cell characterization and analysis -- Cell counting and viability measurements / M. Butler and M. Spearman -- Monitoring of growth, physiology and productivity of animal cells by flow cytometry / S. Carroll, M. Naeiri and M. Al-Rubeai -- Nuclear magnetic resonance methods for monitoring cell growth and metabolism in intensive bioreactors / A.A. Neves and K.M. Brindle -- Methods for off-line analysis of nutrients and products in mammalian cell culture / H. Büntemeyer -- Application of stoichiometric and kinetic analyses to characterize cell growth and product formation / D. Adams, R. Korke, and W.-S. Hu -- Measurement of apoptosis in cell culture / A. Ishaque1 and M. Al-Rubeai -- Metabolic flux estimation in mammalian cell cultures / C.T. Goudar [and others] -- pt. 4. Special cultivation techniques -- Disposable bioreactors for inoculum production and protein expression / R. Eibl and D. Eibl -- Hollow fiber cell culture / J.M. Davis -- Cultivation of mammalian cells in fixed bed reactors / ̦R. Pörtner and O.B.J. Platas Barradas -- Configuration of bioreactors / D.E. Martens and E.J. van den End -- pt. 5. Downstream techniques -- Membrane filtration in animal cell culture / P. Czermak, D. Nehring and R. Wickramasinghe -- Chromatographic techniques in the downstream processing of (recombinant) proteins / R. Freitag -- pt. 6. Special applications -- Vaccine production : State of the art and future needs in upstream processing / Y. Genzel and U. Reichl -- Retrovirus production and characterization / P.E. Cruz [and others] -- Insect cell cultivation and generation of recombinant baculovirus particles for recombinant protein production / S. Geisse.

Print version record.

Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors. Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

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