Adenovirus methods and protocols. Volume 2, Ad proteins, RNA, Lifecycle, Host Interactions, and Phylogenetics / edited by William S.M. Wold, Ann E. Tollefson.

Contributor(s): Wold, William S. M | Tollefson, Ann E
Material type: TextTextSeries: Methods in molecular medicine: 131.Publisher: Totowa, NJ : Humana Press, ©2007Edition: 2nd edDescription: 1 online resource (1 volume) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781597452779; 1597452777; 9781588299017; 1588299015Other title: Ad proteins, RNA, Lifecycle, Host Interactions, and PhylogeneticsSubject(s): Adenoviruses -- Laboratory manuals | Molecular biology -- Laboratory manuals | Adenoviridae | SCIENCE -- Life Sciences -- Biology | SCIENCE -- Life Sciences -- Microbiology | Molecular biology | Adenoviridae | Adenoviruses | Adenoviruses | Molecular biology | adenoviridae | fylogenetica | phylogenetics | rna | moleculaire genetica | molecular genetics | levenscyclus | life cycle | viruseiwitten | viral proteins | biologische technieken | biological techniques | moleculaire virologie | molecular virology | protocollen | protocols | host pathogen interactions | Molecular Biology (General) | Virology | Moleculaire biologie (algemeen) | VirologieGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Adenovirus methods and protocols. Volume 2, Ad proteins, RNA, Lifecycle, Host Interactions, and Phylogenetics.DDC classification: 579.2/443 LOC classification: QR396 | .A336eb vol. 2Other classification: R373-3 Online resources: Click here to access online
Contents:
Preface / William S.M. Wold, Ann E. Tollefson -- Contents of the companion volume -- contributors.
1. Analysis of the efficiency of adenovirus transcription / Cristina Iftode and S.J. Flint -- 2. The Use of in vitro transcription to probe regulatory functions of viral protein domains / Paul M. Loewenstein, Chao-Zhong Song, and Maurice Green -- 3. Preparation of soluble extracts from adenovirus-infected calls for studies of RNA splicing / Oliver Mühlemann and Göran Akusjärvi -- 4. In vitro methods to study RNA interference during an adenovirus infection / Gunnar Andersson, Ning Xu, and Göran Akusjärvi -- 5. Simultaneous detection of adenovirus RNA and cellular proteins by fluorescent labeling in situ / Eileen Bridge -- 6. Study of nucleolar localization of adenovirus core proteins / David A. Matthews -- 7. Analysis of adenovirus infections in synchronized cells / David A. Ornelles, Robin N. Broughton-Shepard, and Felicia D. Goodrum -- 8. Co-Immunoprecipitation of protein complexes / Peter Yaciuk -- 9. Chromatin immunoprecipitation to study the binding of proteins to the adenovirus genome in vivo / Jihong Yang and Patrick Hearing -- 10. Assaying protein-DNA interactions in vivo and in vitro using chromatin immunoprecipitation and electrophoretic mobility shift assays / Pilar Perez-Romero and Michael J. Imperiale -- 11. Identifying functional adenovirus-host interactions using tandem mass spectrometry / Anuj Gaggar, Dmitry Shayakhmetov, and André Lieber -- 12. The Use of cell microinjection for the in vivo analysis of viral transcriptional regulatory protein domains / Maurice Green, Andrew Thorburn, Robert Kern, and Paul M. Loewenstein -- 13. Determination of the transforming activities of adenovirus oncogenes / Michael Nevels and Thomas Dobner -- 14. Human adenovirus type 12: crossing species barriers to immortalize the viral genome / Walter Doerfler -- 15. Measurement of natural-killer cell lytic activity of adenovirus-infected or adenovirus-transformed cells / John M. Routes -- 16. A Flow cytometric assay for analysis of natural-killer cell-mediated cytolysis of adenovirus-transformed cells / Graham Bottley, Graham P. Cook, and G. Eric Blair -- 17. Large-scale purification and crystallization of adenovirus hexon / John J. Rux and Roger M. Burnett -- 18. Synthesis and assay of recombinant adenovirus protease / Joseph M. Weber -- 19. Assay for the adenovirus proteinase: Purification of the enzyme and synthesis of a fluorogenic substrate / Walter F. Mangel and William J. McGrath -- 20. Cofactors of the adenovirus proteinase: Measuring equilibrium dissociation constants and stoichiometries of binding / Walter F. Mangel and William J. McGrath -- 21. Characterization of the adenovirus fiber protein / Jeffrey A. Engler and Jeong Shin Hong -- 22. Phylogenetic analysis of adenovirus sequences / Balázs Harrach and Mária Benko -- Assessment of genetic variability among subspecies B1 human adenoviruses for molecular epidemiology studies / Adriana E. Kajon and Dean D. Erdman -- Index.
In: Springer ProtocolsSummary: Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
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Includes bibliographical references and indexes.

Preface / William S.M. Wold, Ann E. Tollefson -- Contents of the companion volume -- contributors.

1. Analysis of the efficiency of adenovirus transcription / Cristina Iftode and S.J. Flint -- 2. The Use of in vitro transcription to probe regulatory functions of viral protein domains / Paul M. Loewenstein, Chao-Zhong Song, and Maurice Green -- 3. Preparation of soluble extracts from adenovirus-infected calls for studies of RNA splicing / Oliver Mühlemann and Göran Akusjärvi -- 4. In vitro methods to study RNA interference during an adenovirus infection / Gunnar Andersson, Ning Xu, and Göran Akusjärvi -- 5. Simultaneous detection of adenovirus RNA and cellular proteins by fluorescent labeling in situ / Eileen Bridge -- 6. Study of nucleolar localization of adenovirus core proteins / David A. Matthews -- 7. Analysis of adenovirus infections in synchronized cells / David A. Ornelles, Robin N. Broughton-Shepard, and Felicia D. Goodrum -- 8. Co-Immunoprecipitation of protein complexes / Peter Yaciuk -- 9. Chromatin immunoprecipitation to study the binding of proteins to the adenovirus genome in vivo / Jihong Yang and Patrick Hearing -- 10. Assaying protein-DNA interactions in vivo and in vitro using chromatin immunoprecipitation and electrophoretic mobility shift assays / Pilar Perez-Romero and Michael J. Imperiale -- 11. Identifying functional adenovirus-host interactions using tandem mass spectrometry / Anuj Gaggar, Dmitry Shayakhmetov, and André Lieber -- 12. The Use of cell microinjection for the in vivo analysis of viral transcriptional regulatory protein domains / Maurice Green, Andrew Thorburn, Robert Kern, and Paul M. Loewenstein -- 13. Determination of the transforming activities of adenovirus oncogenes / Michael Nevels and Thomas Dobner -- 14. Human adenovirus type 12: crossing species barriers to immortalize the viral genome / Walter Doerfler -- 15. Measurement of natural-killer cell lytic activity of adenovirus-infected or adenovirus-transformed cells / John M. Routes -- 16. A Flow cytometric assay for analysis of natural-killer cell-mediated cytolysis of adenovirus-transformed cells / Graham Bottley, Graham P. Cook, and G. Eric Blair -- 17. Large-scale purification and crystallization of adenovirus hexon / John J. Rux and Roger M. Burnett -- 18. Synthesis and assay of recombinant adenovirus protease / Joseph M. Weber -- 19. Assay for the adenovirus proteinase: Purification of the enzyme and synthesis of a fluorogenic substrate / Walter F. Mangel and William J. McGrath -- 20. Cofactors of the adenovirus proteinase: Measuring equilibrium dissociation constants and stoichiometries of binding / Walter F. Mangel and William J. McGrath -- 21. Characterization of the adenovirus fiber protein / Jeffrey A. Engler and Jeong Shin Hong -- 22. Phylogenetic analysis of adenovirus sequences / Balázs Harrach and Mária Benko -- Assessment of genetic variability among subspecies B1 human adenoviruses for molecular epidemiology studies / Adriana E. Kajon and Dean D. Erdman -- Index.

Print version record.

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.

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