Chemical genomics : reviews and protocols / edited by Edward D. Zanders.

Contributor(s): Zanders, Edward D
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 310.Publisher: Totowa, N.J. : Humana Press, ©2005Description: 1 online resource (xi, 279 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1588293998; 9781588293992; 9781592599486; 1592599486Subject(s): Genomics | Proteomics | Pharmacogenetics | Biochemical genetics | DNA microarrays | Combinatorial chemistry | SCIENCE -- Life Sciences -- Genetics & Genomics | Biochemical genetics | Combinatorial chemistry | DNA microarrays | Genomics | Pharmacogenetics | Proteomics | Genomik | Molekulargenetik | Proteomanalyse | Biology | Health & Biological Sciences | Genetics | Genomics -- methods -- Laboratory Manuals | Combinatorial Chemistry Techniques -- Laboratory Manuals | Ligands -- Laboratory Manuals | Proteins -- chemical synthesis -- Laboratory Manuals | Microarray Analysis | Investigative Techniques | Molecular Probe Techniques | Genetics | Laboratory Chemicals | Publication Formats | Amino Acids, Peptides, and Proteins | Technology, Pharmaceutical | Computational Biology | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Specialty Uses of Chemicals | Biology | Genetic Techniques | Microchip Analytical Procedures | Publication Characteristics | Chemicals and Drugs | Chemical Actions and Uses | Chemistry Techniques, Analytical | Biological Science Disciplines | Natural Science Disciplines | Disciplines and Occupations | Methods | Genomics | Protein Array Analysis | Combinatorial Chemistry Techniques | Proteins | Molecular Probes | Laboratory Manuals | Protein Array Analysis -- methods -- Laboratory Manuals | LigandsGenre/Form: Electronic books. | Laboratory Manual. Additional physical formats: Print version:: Chemical genomics.DDC classification: 572.8/6 LOC classification: QH431 | .C45197 2005Online resources: Click here to access online
Contents:
Introduction to chemical genomics / Paul R. Caron -- Chemistry for chemical genomics / Lutz Weber -- Computer-aided design of small molecules for chemical genomics / Philip M. Dean -- Design, synthesis, and screening of biomimetic ligands for affinity chromatography / Ana Cecília A. Roque, Geeta Gupta, and Christopher R. Lowe -- The role and application of in silico docking in chemical genomics research / Aldo Jongejan [and others] -- Synthesis of complex carbohydrates and glyconjugates: enzymatic synthesis of globotetraose using 1,3-N-acetylgalactosaminyltransferase LgtD from Haemophilus infuenzae strain Rd / Kang Ryu [and others] -- High-throughput cloning for proteomics research / Sharon A. Doyle.
Screening for the expression of soluble recombinant protein in Escherichia coli / Sharon A. Doyle -- High-throughput purification of hexahistidine-tagged proteins expressed in Escherichia coli / Michael B. Murphy and Sharon A. Doyle -- The wheat germ cell-free expression system: methods for high-throughput materialization of genetic information / Tatsuya Sawasaki [and others] -- Advances in genome-wide protein expression using the wheat germ cell-free system / Yaeta Endo and Tatsuya Sawasaki -- Production of proteins for NMR studies using the wheat germ cell-free system / Toshiyuki Kohno -- Adenoviral expression of reporter proteins for high-throughput cell-based screening / Jon Hoyt and Randall W. King.
Summary: Chemical genomics is an exciting new field that promises to transform biological chemistry into a high-throughput industrialized process, much in the same way that genomics has transformed molecular biology. In Chemical Genomics: Reviews and Protocols, leading edge researchers introduce the uninitiated into the various "flavors" of chemical genomics and describe a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, with emphasis on the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches, to protein-small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Chemical Genomics: Reviews and Protocols offers researchers an accessible introduction by pioneers to the key areas and methods of chemical genomics, as well as its use in the discovery and evaluation of novel drug targets.
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Includes bibliographical references and index.

Introduction to chemical genomics / Paul R. Caron -- Chemistry for chemical genomics / Lutz Weber -- Computer-aided design of small molecules for chemical genomics / Philip M. Dean -- Design, synthesis, and screening of biomimetic ligands for affinity chromatography / Ana Cecília A. Roque, Geeta Gupta, and Christopher R. Lowe -- The role and application of in silico docking in chemical genomics research / Aldo Jongejan [and others] -- Synthesis of complex carbohydrates and glyconjugates: enzymatic synthesis of globotetraose using 1,3-N-acetylgalactosaminyltransferase LgtD from Haemophilus infuenzae strain Rd / Kang Ryu [and others] -- High-throughput cloning for proteomics research / Sharon A. Doyle.

Screening for the expression of soluble recombinant protein in Escherichia coli / Sharon A. Doyle -- High-throughput purification of hexahistidine-tagged proteins expressed in Escherichia coli / Michael B. Murphy and Sharon A. Doyle -- The wheat germ cell-free expression system: methods for high-throughput materialization of genetic information / Tatsuya Sawasaki [and others] -- Advances in genome-wide protein expression using the wheat germ cell-free system / Yaeta Endo and Tatsuya Sawasaki -- Production of proteins for NMR studies using the wheat germ cell-free system / Toshiyuki Kohno -- Adenoviral expression of reporter proteins for high-throughput cell-based screening / Jon Hoyt and Randall W. King.

Print version record.

Chemical genomics is an exciting new field that promises to transform biological chemistry into a high-throughput industrialized process, much in the same way that genomics has transformed molecular biology. In Chemical Genomics: Reviews and Protocols, leading edge researchers introduce the uninitiated into the various "flavors" of chemical genomics and describe a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, with emphasis on the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches, to protein-small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Chemical Genomics: Reviews and Protocols offers researchers an accessible introduction by pioneers to the key areas and methods of chemical genomics, as well as its use in the discovery and evaluation of novel drug targets.

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