Pharmacogenomics : methods and protocols / edited by Federico Innocenti.

Contributor(s): Innocenti, Federico
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 311.Publisher: Totowa, N.J. : Humana Press, ©2005Description: 1 online resource (x, 214 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592599578; 1592599575Subject(s): Pharmacogenomics | Pharmacogenetics -- methods | Genomics -- methods | Genetic Variation | Pharmacogénomique | MEDICAL -- Pharmacology | Pharmacogenomics | farmacie | pharmacy | farmacologie | pharmacology | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Pharmacogenomics.DDC classification: 615/.7 LOC classification: RM301.3.G45 | P427 2005Online resources: Click here to access online
Contents:
Pharmacogenomics : historical perspective and current status / Werner Kalow -- Transfection assays with allele-specific constructs : functional analysis of UDP-glucuronosyltransferase variants / Hideto Jinno [and others] -- Snapshot of the allele-specific variation in human gene expression / Hai Yan -- Genome-wide analysis of allele-specific gene expression using oligo microarrays / Maxwell P. Lee -- HaploChIP : an in vivo assay / Julian Charles Knight -- Aspects influencing genotyping method selection / Peter Imle -- Denaturing high-performance liquid chromatography for mutation detection and genotyping / Donna Lee Fackenthal, Pei Xian Chen, and Soma Das.
Pyrosequencing of clinically relevant polymorphisms / Sharon Marsh [and others] -- Kinetic fluorescence-quenching detection assay for allele frequency estimation / Ming Xiao and Pui-Yan Kwok -- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry / Matthias Wjst and Dirk van den Boom -- Fluorescence-based fragment size analysis / Peter Imle -- Single-nucleotide polymorphism genotyping in deoxyribonucleic acid pools / Ian Craig [and others] -- TaqMan genotyping of insertion/deletion polymorphisms / Renato Robledo, William R. Beggs, and Patrick K. Bender -- PharmGKB : the pharmacogenetics and pharmacogenomics knowledge base / Caroline F. Thorn, Teri E. Klein, and Russ B. Altman -- Systems for the management of pharmacogenomic information / Alexander Sturn [and others].
Summary: Pharmacogenomics holds the promise of drugs tailor-made to an individual's genetic makeup for greater efficacy and safety. In Pharmacogenomics: Methods and Protocols, expert researchers and physician/clinicians describe in detail the newest and most common technologies now used in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromatography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Pharmacogenomics: Methods and Protocols offers pharmacologists, geneticists, molecular biologists, and clinical chemists working in academia, biotechnology, and the pharmaceutical industry a cutting-edge collection of the key techniques used for today's preclinical and clinical studies.
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Includes bibliographical references and index.

Pharmacogenomics : historical perspective and current status / Werner Kalow -- Transfection assays with allele-specific constructs : functional analysis of UDP-glucuronosyltransferase variants / Hideto Jinno [and others] -- Snapshot of the allele-specific variation in human gene expression / Hai Yan -- Genome-wide analysis of allele-specific gene expression using oligo microarrays / Maxwell P. Lee -- HaploChIP : an in vivo assay / Julian Charles Knight -- Aspects influencing genotyping method selection / Peter Imle -- Denaturing high-performance liquid chromatography for mutation detection and genotyping / Donna Lee Fackenthal, Pei Xian Chen, and Soma Das.

Pyrosequencing of clinically relevant polymorphisms / Sharon Marsh [and others] -- Kinetic fluorescence-quenching detection assay for allele frequency estimation / Ming Xiao and Pui-Yan Kwok -- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry / Matthias Wjst and Dirk van den Boom -- Fluorescence-based fragment size analysis / Peter Imle -- Single-nucleotide polymorphism genotyping in deoxyribonucleic acid pools / Ian Craig [and others] -- TaqMan genotyping of insertion/deletion polymorphisms / Renato Robledo, William R. Beggs, and Patrick K. Bender -- PharmGKB : the pharmacogenetics and pharmacogenomics knowledge base / Caroline F. Thorn, Teri E. Klein, and Russ B. Altman -- Systems for the management of pharmacogenomic information / Alexander Sturn [and others].

Print version record.

Pharmacogenomics holds the promise of drugs tailor-made to an individual's genetic makeup for greater efficacy and safety. In Pharmacogenomics: Methods and Protocols, expert researchers and physician/clinicians describe in detail the newest and most common technologies now used in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromatography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Pharmacogenomics: Methods and Protocols offers pharmacologists, geneticists, molecular biologists, and clinical chemists working in academia, biotechnology, and the pharmaceutical industry a cutting-edge collection of the key techniques used for today's preclinical and clinical studies.

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