Fibrosis research : methods and protocols / edited by John Varga, David A. Brenner, Sem H. Phan.

Contributor(s): Varga, John | Brenner, David, 1953- | Phan, Sem Hin, 1949-
Material type: TextTextSeries: Methods in molecular medicine: 117.Publisher: Totowa, N.J. : Humana Press, ©2005Description: 1 online resource (xvi, 392 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592599400; 1592599400; 158829479X; 9781588294791Subject(s): Fibrosis | Fibrosis -- Research -- Methodology | Fibrosis | Cells, Cultured | Extracellular Matrix -- metabolism | Models, Animal | Fibrose | Fibrose -- Recherche -- Méthodologie | MEDICAL -- Orthopedics | HEALTH & FITNESS -- Diseases -- Musculoskeletal | MEDICAL -- Rheumatology | Fibrosis | geneeskunde | medicine | Medicine (General) | Geneeskunde (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Fibrosis research.DDC classification: 616.7/42 LOC classification: RB150.F52 | F54 2005ebOnline resources: Click here to access online
Contents:
Pulmonary fibrosis / David A. Zisman [and others] -- Renal fibrosis / H. William Schnaper -- Transforming growth factor-[beta] : a key mediator of fibrosis / Alain Mauviel -- Isolation and culture of skin fibroblasts / Laure Rittié and Gary J. Fisher -- Isolation and culture of hepatic stellate cells / Ralf Weiskirchen and Axel M. Gressner -- Isolation and phenotypic characterization of lung fibroblasts / Carolyn J. Baglole [and others] -- Methods for measuring typo I collagen synthesis in vitro / David C. Rishikof [and others] -- Methods for assessing the molecular mechanisms controlling gene regulation / Richard A. Rippe and Branko Stefanovic -- Methods for measuring TGF-[beta] using antibodies, cells, and mice / Vladimir Jurukovski [and others] -- Morphological methods for assessment of fibrosis / Rakesh K. Kumar -- Methods for measuring hydroxyproline and estimating in vivo rates of collagen synthesis and degradation / Robin J. McAnulty.
Approaches to evaluation of fibrogenic pathways in surgical lung biopsy specimens / Cory M. Hogaboam [and others] -- Animal models for adult dermal wound healing / Mary Birch, Anette Tomlinson, and Mark W.J. Ferguson -- Modeling liver fibrosis in rodents / Christothea Constandinou, Neil Henderson, and John P. Iredale -- Animal models of pulmonary fibrosis / Mehrnaz Gharaee-Kermani, Matthew Ullenbruch, and Sem H. Phan -- Animal models of renal fibrosis / Michael Zeisberg, Mary A. Soubasakos, and Raghu Kalluri -- Animal models of cardiac fibrosis / Yao Sun and Karl T. Weber -- Finding fibrosis genes : the lung / Lauranell H. Burch and David A. Schwartz -- Genetic studies to identify hepatic fibrosis genes and SNPs in human populations / Christopher Paul Day -- Analysis of microarray experiments for pulmonary fibrosis / Nilesh B. Davé and Naftali Kaminski -- DNA microarrays and data mining to study hepatic fibrosis / Bernd Schnabl [and others] -- Analysis of proteins dominantly expressed in hepatic stellate cells of activated phenotype : the molecular approach for liver fibrosis / Norifumi Kawada.
Summary: Fibrosis, or scar tissue, is increasingly recognized as an important feature of many chronic diseases of the lung, kidney, heart, skin, eye, and bone. With some 45% of deaths in the United States attributed to fibrosing disorders and the lack of any effective treatment, research into the cellular, molecular, and genetic basis of fibrosis has exploded. In Fibrosis Research: Methods and Protocols, leading investigators at the forefront of this emerging field review the highlights of current fibrosis work in a variety of disciplines and the experimental methodologies used to uncover the mechanisms that drive it. In their discussion of research techniques utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. Additional techniques presented cover the evolving genetic methods for identifying "fibrosis genes" or allelic polymorphisms in human populations, microarray studies for describing global patterns of gene expression associated with fibrosis, and proteomic approaches to the same. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Fibrosis Research: Methods and Protocols offers investigators all the powerful experimental methodologies, techniques, and strategies needed today to study and ultimately understand the daunting problem of pathological fibrosis.
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Includes bibliographical references and index.

Pulmonary fibrosis / David A. Zisman [and others] -- Renal fibrosis / H. William Schnaper -- Transforming growth factor-[beta] : a key mediator of fibrosis / Alain Mauviel -- Isolation and culture of skin fibroblasts / Laure Rittié and Gary J. Fisher -- Isolation and culture of hepatic stellate cells / Ralf Weiskirchen and Axel M. Gressner -- Isolation and phenotypic characterization of lung fibroblasts / Carolyn J. Baglole [and others] -- Methods for measuring typo I collagen synthesis in vitro / David C. Rishikof [and others] -- Methods for assessing the molecular mechanisms controlling gene regulation / Richard A. Rippe and Branko Stefanovic -- Methods for measuring TGF-[beta] using antibodies, cells, and mice / Vladimir Jurukovski [and others] -- Morphological methods for assessment of fibrosis / Rakesh K. Kumar -- Methods for measuring hydroxyproline and estimating in vivo rates of collagen synthesis and degradation / Robin J. McAnulty.

Approaches to evaluation of fibrogenic pathways in surgical lung biopsy specimens / Cory M. Hogaboam [and others] -- Animal models for adult dermal wound healing / Mary Birch, Anette Tomlinson, and Mark W.J. Ferguson -- Modeling liver fibrosis in rodents / Christothea Constandinou, Neil Henderson, and John P. Iredale -- Animal models of pulmonary fibrosis / Mehrnaz Gharaee-Kermani, Matthew Ullenbruch, and Sem H. Phan -- Animal models of renal fibrosis / Michael Zeisberg, Mary A. Soubasakos, and Raghu Kalluri -- Animal models of cardiac fibrosis / Yao Sun and Karl T. Weber -- Finding fibrosis genes : the lung / Lauranell H. Burch and David A. Schwartz -- Genetic studies to identify hepatic fibrosis genes and SNPs in human populations / Christopher Paul Day -- Analysis of microarray experiments for pulmonary fibrosis / Nilesh B. Davé and Naftali Kaminski -- DNA microarrays and data mining to study hepatic fibrosis / Bernd Schnabl [and others] -- Analysis of proteins dominantly expressed in hepatic stellate cells of activated phenotype : the molecular approach for liver fibrosis / Norifumi Kawada.

Print version record.

Fibrosis, or scar tissue, is increasingly recognized as an important feature of many chronic diseases of the lung, kidney, heart, skin, eye, and bone. With some 45% of deaths in the United States attributed to fibrosing disorders and the lack of any effective treatment, research into the cellular, molecular, and genetic basis of fibrosis has exploded. In Fibrosis Research: Methods and Protocols, leading investigators at the forefront of this emerging field review the highlights of current fibrosis work in a variety of disciplines and the experimental methodologies used to uncover the mechanisms that drive it. In their discussion of research techniques utilizing cultured cells to model various aspects of the fibrotic response in vitro, the authors describe the isolation, characterization, and propagation of mesenchymal cells, and highlight the similarities and differences between methods that are appropriate for different types of fibroblasts. Approaches for studying collagen gene regulation and TGF-b production are also discussed, along with experimental methodologies utilizing animal models to study the pathogenesis of fibrosis. Additional techniques presented cover the evolving genetic methods for identifying "fibrosis genes" or allelic polymorphisms in human populations, microarray studies for describing global patterns of gene expression associated with fibrosis, and proteomic approaches to the same. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Fibrosis Research: Methods and Protocols offers investigators all the powerful experimental methodologies, techniques, and strategies needed today to study and ultimately understand the daunting problem of pathological fibrosis.

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