Microchip capillary electrophoresis : methods and protocols / edited by Charles S. Henry.

Contributor(s): Henry, Charles S. (Charles Sherman), 1972-
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 339.Publisher: Totowa, N.J. : Humana Press, ©2006Description: 1 online resource (x, 237 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 1588292932; 1597450766; 9781597450768; 9781588292933; 1280832118; 9781280832116Subject(s): Capillary electrophoresis | Integrated circuits | Electrophoresis, Microchip | Electrophoresis, Capillary | Microfluidic Analytical Techniques | Microchip Analytical Procedures | Electrophoresis | Chemistry Techniques, Analytical | Investigative Techniques | Analytical, Diagnostic and Therapeutic Techniques and Equipment | SCIENCE -- Life Sciences -- Molecular Biology | Capillary electrophoresis | Integrated circuitsGenre/Form: Electronic books. Additional physical formats: Print version:: Microchip capillary electrophoresis.DDC classification: 572.8028 LOC classification: QP519.9.C36 | M53 2006Online resources: Click here to access online
Contents:
Microchip capilllary electrophoresis: an introduction / Charles S. Henry -- Fabrication of a glass capillary electrophoresis microchip with integrated electrodes / Mark M. Crain [and others] -- Micro-molding for poly(dimethylsiloxane) microchips / Carlos D. Garcia and Charles S. Henry -- Fabrication of polymer microfluidic systems by hot embossing and laser ablation / Laurie E. Locascio [and others] -- Surface modification methods for enhanced device efficacy and function / Barbara J. Jones and Mark A. Hayes -- Polyelectrolyte coatings for microchip capillary electrophoresis / Yan Liu and Charles S. Henry.
Interfacing microchip capillary electrophoresis with electrospray ionization mass spectrometry/ Trust Razunguzwa and Aaron T. Timperman -- Interfacing amperometric detection with microchip capillary electrophoresis / R. Scott Martin -- Conductivity detection on microchips / Roland Hergenröder and Benedikt Grass -- DNA separations / Andrea W. Chow -- Protein separations / Andrea W. Chow -- Microchip capillary electrophoresis: application to peptide analysis / Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte -- Measuring electroosmotic flow in microchips and capillaries / S. Douglass Gilman and Peter J. Chapman -- Single cell analysis on microfluidic devices / Christopher T. Culbertson -- Rapid DNA amplification in glass microdevices / Christopher J. Easley [and others].
Summary: Microchip capillary electrophoresis, a new and emerging technology that can rapidly analyze biological compounds as small as or smaller than a single cell, will have a major impact on biological sciences in the next 10 to 20 years. In Microchip Capillary Electrophoresis: Methods and Protocols, leading chemists and engineers concisely explain the principles behind this revolutionary technique and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. The authors describe in detail the detection methods used, including mass spectrometry, electrochemistry, and conductivity. Since surface chemistry must be carefully considered for optimal operation at this scale, they also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Instructive and well-detailed, Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for both initiating research in the field of microchip capillary electrophoresis and understanding the full range of how these new systems can be used successfully in biological research.
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Includes bibliographical references and index.

Microchip capilllary electrophoresis: an introduction / Charles S. Henry -- Fabrication of a glass capillary electrophoresis microchip with integrated electrodes / Mark M. Crain [and others] -- Micro-molding for poly(dimethylsiloxane) microchips / Carlos D. Garcia and Charles S. Henry -- Fabrication of polymer microfluidic systems by hot embossing and laser ablation / Laurie E. Locascio [and others] -- Surface modification methods for enhanced device efficacy and function / Barbara J. Jones and Mark A. Hayes -- Polyelectrolyte coatings for microchip capillary electrophoresis / Yan Liu and Charles S. Henry.

Interfacing microchip capillary electrophoresis with electrospray ionization mass spectrometry/ Trust Razunguzwa and Aaron T. Timperman -- Interfacing amperometric detection with microchip capillary electrophoresis / R. Scott Martin -- Conductivity detection on microchips / Roland Hergenröder and Benedikt Grass -- DNA separations / Andrea W. Chow -- Protein separations / Andrea W. Chow -- Microchip capillary electrophoresis: application to peptide analysis / Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte -- Measuring electroosmotic flow in microchips and capillaries / S. Douglass Gilman and Peter J. Chapman -- Single cell analysis on microfluidic devices / Christopher T. Culbertson -- Rapid DNA amplification in glass microdevices / Christopher J. Easley [and others].

Print version record.

Microchip capillary electrophoresis, a new and emerging technology that can rapidly analyze biological compounds as small as or smaller than a single cell, will have a major impact on biological sciences in the next 10 to 20 years. In Microchip Capillary Electrophoresis: Methods and Protocols, leading chemists and engineers concisely explain the principles behind this revolutionary technique and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. The authors describe in detail the detection methods used, including mass spectrometry, electrochemistry, and conductivity. Since surface chemistry must be carefully considered for optimal operation at this scale, they also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Instructive and well-detailed, Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for both initiating research in the field of microchip capillary electrophoresis and understanding the full range of how these new systems can be used successfully in biological research.

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