Epidermal growth factor : methods and protocols / edited by Tarun B. Patel, Paul J. Bertics.

Contributor(s): Patel, Tarun B, 1953- | Bertics, Paul J
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 327.Publisher: Totowa, N.J. : Humana Press, ©2006Description: 1 online resource (xi, 208 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 1588294218; 9781588294210; 9781597450126; 159745012XSubject(s): Epidermal growth factor -- Laboratory manuals | Epidermal Growth Factor -- analysis | Epidermal Growth Factor -- physiology | ErbB Receptors -- analysis | ErbB Receptors -- physiology | Epidermal Growth Factor | Physiology | ErbB Receptors | Intercellular Signaling Peptides and Proteins | Receptors, Growth Factor | Gastrointestinal Hormones | Receptor Protein-Tyrosine Kinases | Receptors, Gastrointestinal Hormone | Biological Science Disciplines | Receptors, Peptide | Natural Science Disciplines | Protein-Tyrosine Kinases | Proteins | Hormones | Peptides | Receptors, Cell Surface | Biological Factors | Disciplines and Occupations | Membrane Proteins | Hormones, Hormone Substitutes, and Hormone Antagonists | Protein Kinases | Amino Acids, Peptides, and Proteins | Chemicals and Drugs | Phosphotransferases (Alcohol Group Acceptor) | Phosphotransferases | Transferases | Enzymes | Enzymes and Coenzymes | MEDICAL -- Physiology | SCIENCE -- Life Sciences -- Human Anatomy & Physiology | Epidermal growth factorGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Epidermal growth factor.DDC classification: 612.7/91 LOC classification: QP552.E59 | E47 2006Online resources: Click here to access online
Contents:
A historical perspective of EGFR and related systems / Francis Edwin [and others] -- Purification and assay of kinase-active EGF receptor from mammalian cells by immunoaffinity chromatography / Gregory J. Wiepz [and others] -- Soluble epidermal growth factor receptor acrydinium-linked immunosorbent assay / Jacqueline Lafky, Andre T. Baron, and Nita J. Maihle -- Analysis of EGF receptor and interactions with the subunit of the stimulatory GTP binding protein of adenylyl cyclase, Gs / Deepti Chaturvedi [and others] -- Regulator of epiderma growth factor signaling: sprouty / Esther Sook Miin Wong and Graeme R. Guy -- Dissecting the epidermal growth factor receptor signal transactivation pathway / Oliver M. Fischer, Stefan Hart, and Axel Ullrich -- A sensitive method to monitor ectodomain shedding of ligands of the epidermal growth factor receptor / Umut Sahin [and others] -- In vitro and in vivo assays of monoubiquitnation of receptor tyrosine kinases / Manachem Katz, Yaron Mosesson, and Yosef Yarden -- Assays to monitor degradation of the EGF receptor / Mirko H.H. Schmidt and Ivan Dikic -- Assessment of degradation and ubiquitination of CXCR4, a GPCR regulated by EGFR family members / Adriano Marchese -- Epithelial cell migration in response to epidermal growth factor / Reema Zeineldin and Laurie G. Hudson -- Motility signaled from EGF receptors and related systems / Alan Wells [and others] -- Methods for determining the proliferation of cells in response to EGFR ligands / Gregory J. Wiepz [and others] -- Clinical advancement of EGFR inhibitors in cancer therapy / Prakash Chinnaiyan and Paul M. Harari.
Summary: Epidermal growth factor (EGF) and related molecules regulate a wide array of biological events, ranging from normal growth and development to such pathological states as cancer and cardiovascular diseases. In Epidermal Growth Factor: Methods and Protocols, expert researchers active in the field present their best methods to dissect the mechanisms that control growth factors and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Epidermal Growth Factor: Methods and Protocols offers investigators an array of powerful and proven tools to unravel the workings of epidermal growth factor, as well as develop new strategies for the successful use of this important developmental and therapeutic target.
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Includes bibliographical references and index.

A historical perspective of EGFR and related systems / Francis Edwin [and others] -- Purification and assay of kinase-active EGF receptor from mammalian cells by immunoaffinity chromatography / Gregory J. Wiepz [and others] -- Soluble epidermal growth factor receptor acrydinium-linked immunosorbent assay / Jacqueline Lafky, Andre T. Baron, and Nita J. Maihle -- Analysis of EGF receptor and interactions with the subunit of the stimulatory GTP binding protein of adenylyl cyclase, Gs / Deepti Chaturvedi [and others] -- Regulator of epiderma growth factor signaling: sprouty / Esther Sook Miin Wong and Graeme R. Guy -- Dissecting the epidermal growth factor receptor signal transactivation pathway / Oliver M. Fischer, Stefan Hart, and Axel Ullrich -- A sensitive method to monitor ectodomain shedding of ligands of the epidermal growth factor receptor / Umut Sahin [and others] -- In vitro and in vivo assays of monoubiquitnation of receptor tyrosine kinases / Manachem Katz, Yaron Mosesson, and Yosef Yarden -- Assays to monitor degradation of the EGF receptor / Mirko H.H. Schmidt and Ivan Dikic -- Assessment of degradation and ubiquitination of CXCR4, a GPCR regulated by EGFR family members / Adriano Marchese -- Epithelial cell migration in response to epidermal growth factor / Reema Zeineldin and Laurie G. Hudson -- Motility signaled from EGF receptors and related systems / Alan Wells [and others] -- Methods for determining the proliferation of cells in response to EGFR ligands / Gregory J. Wiepz [and others] -- Clinical advancement of EGFR inhibitors in cancer therapy / Prakash Chinnaiyan and Paul M. Harari.

Print version record.

Epidermal growth factor (EGF) and related molecules regulate a wide array of biological events, ranging from normal growth and development to such pathological states as cancer and cardiovascular diseases. In Epidermal Growth Factor: Methods and Protocols, expert researchers active in the field present their best methods to dissect the mechanisms that control growth factors and their regulators in both normal and pathological states. These readily reproducible techniques range from the study of purified EGF receptor to complex signaling and processing networks in intact cells, including a chapter on the clinical and pharmacological considerations of their use in cancer therapy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Epidermal Growth Factor: Methods and Protocols offers investigators an array of powerful and proven tools to unravel the workings of epidermal growth factor, as well as develop new strategies for the successful use of this important developmental and therapeutic target.

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