New and emerging proteomic techniques / edited by Dobrin Nedelkov and Randall W. Nelson.

Contributor(s): Nedelkov, Dobrin | Nelson, Randall W
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 328.Publisher: Totowa, N.J. : Humana Press, ©2006Description: 1 online resource (xi, 232 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 1588295192; 159745026X; 9781597450263; 9781588295194Subject(s): Proteomics -- Laboratory manuals | Proteomics -- methods | Chemistry, Analytical -- methods | Computational Biology -- methods | Genetic Techniques | Proteomics | Computational Biology | Chemistry Techniques, Analytical | Methods | Investigative Techniques | Biology | Genomics | Biochemistry | Publication Formats | Biological Science Disciplines | Publication Characteristics | Genetics | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Chemistry | Natural Science Disciplines | Disciplines and Occupations | SCIENCE -- Life Sciences -- Biochemistry | ProteomicsGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: New and emerging proteomic techniques.DDC classification: 572/.6/078 LOC classification: QP551 | .N49 2006Online resources: Click here to access online
Contents:
On-chip protein synthesis for making microarrays / Niroshan Ramachandran [and others] -- RCA-enhanced protein detection arrays / Brian B. Haab and Paul M. Lizardi -- Antibody microarrays using resonance light-scattering particles for detection / Bernhard H. Geierstanger, Petri Saviranta, and Achim Brinker -- Chemical proteomics profiling of proteasome activity / Martijn Verdoes [and others] -- Two-dimensional difference gel electrophoresis / Terence L. Wu -- Oligomeric states of proteins determined by size-exclusion chromatography coupled with light scattering, absorbance, and refractive index detectors / Ewa Folta-Stogniew -- Surface plasmon resonance imaging measurements of protein interactions with biopolymer microarrays / Terry T. Goodrich [and others].
Surface plasmon resonance mass spectrometry for protein analysis / Dobrin Nedelkov and Randall W. Nelson -- High-throughput affinity mass spectrometry / Urban A. Kiernan [and others] -- Isotope-coded affinity tags for protein quantification / Christopher M. Colangelo and Kenneth R. Williams -- Proteomic analysis by multidimensional protein identification technology / Laurence Florens and Michael P. Washburn -- Isolation of glycoproteins and identification of their N-linked glycosylation sites / Hui Zhang and Ruedi Aebersold -- N-glycosylation analysis using the StrOligo algorithm / Martin Ethier, Daniel Figeys, and Hélène Perreault -- MALDI-MS data analysis for disease biomarker discovery / Weichuan Yu [and others] -- Using the global proteome machine for protein identification / Ronald C. Beavis.
Summary: Numerous emerging technologies have propelled the growth of proteomics into a wide-ranging discipline with great potential to improve human health through the discovery of new protein biomarkers and therapeutics. In New and Emerging Proteomic Techniques, a panel of leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods described here span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. Where necessary, two or more distinct techniques are provided for a given problem, and advanced bioinformatics approaches are presented for the analysis of complex proteomic data. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, New and Emerging Proteomic Techniques offers expert and novice investigators alike today's most promising proteomics techniques for probing the biological processes and understanding the diseases of the human body.
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Includes bibliographical references and index.

On-chip protein synthesis for making microarrays / Niroshan Ramachandran [and others] -- RCA-enhanced protein detection arrays / Brian B. Haab and Paul M. Lizardi -- Antibody microarrays using resonance light-scattering particles for detection / Bernhard H. Geierstanger, Petri Saviranta, and Achim Brinker -- Chemical proteomics profiling of proteasome activity / Martijn Verdoes [and others] -- Two-dimensional difference gel electrophoresis / Terence L. Wu -- Oligomeric states of proteins determined by size-exclusion chromatography coupled with light scattering, absorbance, and refractive index detectors / Ewa Folta-Stogniew -- Surface plasmon resonance imaging measurements of protein interactions with biopolymer microarrays / Terry T. Goodrich [and others].

Surface plasmon resonance mass spectrometry for protein analysis / Dobrin Nedelkov and Randall W. Nelson -- High-throughput affinity mass spectrometry / Urban A. Kiernan [and others] -- Isotope-coded affinity tags for protein quantification / Christopher M. Colangelo and Kenneth R. Williams -- Proteomic analysis by multidimensional protein identification technology / Laurence Florens and Michael P. Washburn -- Isolation of glycoproteins and identification of their N-linked glycosylation sites / Hui Zhang and Ruedi Aebersold -- N-glycosylation analysis using the StrOligo algorithm / Martin Ethier, Daniel Figeys, and Hélène Perreault -- MALDI-MS data analysis for disease biomarker discovery / Weichuan Yu [and others] -- Using the global proteome machine for protein identification / Ronald C. Beavis.

Print version record.

Numerous emerging technologies have propelled the growth of proteomics into a wide-ranging discipline with great potential to improve human health through the discovery of new protein biomarkers and therapeutics. In New and Emerging Proteomic Techniques, a panel of leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods described here span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. Where necessary, two or more distinct techniques are provided for a given problem, and advanced bioinformatics approaches are presented for the analysis of complex proteomic data. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, New and Emerging Proteomic Techniques offers expert and novice investigators alike today's most promising proteomics techniques for probing the biological processes and understanding the diseases of the human body.

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