Plant cell culture protocols / edited by Victor M. Loyola-Vargas and Felipe Vázquez-Flota.

Contributor(s): Loyola-Vargas, Victor M | Vázquez-Flota, Felipe
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 318.Publisher: Totowa, N.J. : Humana Press, ©2006Edition: 2nd edDescription: 1 online resource (xv, 393 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 1588295478; 9781588295477; 9781592599592; 1592599591Subject(s): Plant cell culture -- Laboratory manuals | Plant tissue culture -- Laboratory manuals | Plant Cells | Cell Culture Techniques | Cells, Cultured | Culture Techniques | Cells | Clinical Laboratory Techniques | Anatomy | Investigative Techniques | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Plantes -- Cellules et tissus -- Culture -- Manuels de laboratoire | SCIENCE -- Life Sciences -- Cell Biology | Plant cell culture | Plant tissue cultureGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Plant cell culture protocols.DDC classification: 571.6/382 LOC classification: QK725 | .P5535 2006Online resources: Click here to access online
Contents:
An introduction to plant cell culture: Back to the future / V.M. Loyola-Vargas and F. Vazquez-Flota -- History of plant tissue culture / T.A. Thorpe -- Pathogen and biological contamination management: the road ahead / A.C. Cassells and B.M. Doyle -- Growth measurements: estimation of cell division and cell expansion / G. Godoy-Hernandez and F.A. Vazquez-Flota -- Callus and suspension culture induction, maintenance, and characterization / A. Robledo-Paz [and others] -- Measurement of cell viability in in vitro cultures / L.A. Castro-Concha, R.M. Escobedo and M.L. Miranda-Ham -- Cryopreservation of embryogenic cell suspensions by encapsulation-vitrification / Q. Wang and A. Perl -- Somatic embryogenesis in Picea suspension cultures / C. Stasolla -- Indirect somatic embryogenesis in cassava for genetic modification purposes / K. Raemakers [and others] -- Direct somatic embryogenesis in Coffea canephora / F. Quiroz-Figueroa [and others] -- A new temporary immersion bioreactor system for micropropagation / M.L. Robert [and others] -- Protocol to achieve photoautotrophic coconut plants cultured in vitro with improved performance ex vitro / G. Fuentes [and others] -- Use of statistics in plant biotechnology / M.E. Compton -- An efficient method for the micropropagation of Agave species / M.L. Robert [and others] -- Micropropagation of endangered plant species / Z. Liao [and others] -- Clonal propagation of softwoods / T.A. Thorpe, I.S. Harry and E.C. Yeung -- Isolation, culture, and plant regeneration from leaf protoplasts of Passiflora / M.R. Davey [and others] -- Isolation, culture, and plant regeneration from Echinacea purpurea protoplasts / Z.G. Pan [and others] -- Production of cybrids in Brassicaceae species / M. Vasylenko [and others] -- Guard cell protoplasts: isolation, culture, and regeneration of plants / G. Tallman -- Production of interspecific hybrid plants in Primula / J. Kato and M. Mii -- Agrobacterium-mediated transformation of Petunia leaf discs / I.M. van der Meer -- Transformation of wheat via particle bombardment / I.K. Vasil and V. Vasil -- Chloroplast transformation / X.M. Lu, W.B. Yin and Z.M. Hu -- The biochemical basis for the resistance to aluminum and their potential as selection markers / S.B. De los and T. Hernandez-Sotomayor -- Transformation of maize via Agrobacterium tumefaciens using a binary co-integrate vector system / Z.Y. Zhao and J. Ranch -- Capsaicin accumulation in Capsicum spp. suspension cultures / N. Ochoa-Alejo -- Isolation and purification of ribosome-inactivating proteins / S.W. Park [and others] -- Catharanthus roseus shoot cultures for the production of monoterpenoid indole alkaloids / E. Hernandez-Dominguez [and others] -- Methods for regeneration and transformation in Eschscholzia californica: A model plant to investigate alkaloid biosynthesis / B.P. MacLeod and P.J. Facchini -- The components of the culture media / V.M. Loyola-Vargas -- Plant biotechnology and tissue culture resources on the internet / V.M. Loyola-Vargas.
Summary: Following in the footsteps of its much-acclaimed first edition, Plant Cell Culture Protocols, Second Edition has been expanded and revised to include the most up-to-date and frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Two new appendices have also been added to explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, as well as to list more than 100 very useful internet sites. Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications.
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Includes bibliographical references.

An introduction to plant cell culture: Back to the future / V.M. Loyola-Vargas and F. Vazquez-Flota -- History of plant tissue culture / T.A. Thorpe -- Pathogen and biological contamination management: the road ahead / A.C. Cassells and B.M. Doyle -- Growth measurements: estimation of cell division and cell expansion / G. Godoy-Hernandez and F.A. Vazquez-Flota -- Callus and suspension culture induction, maintenance, and characterization / A. Robledo-Paz [and others] -- Measurement of cell viability in in vitro cultures / L.A. Castro-Concha, R.M. Escobedo and M.L. Miranda-Ham -- Cryopreservation of embryogenic cell suspensions by encapsulation-vitrification / Q. Wang and A. Perl -- Somatic embryogenesis in Picea suspension cultures / C. Stasolla -- Indirect somatic embryogenesis in cassava for genetic modification purposes / K. Raemakers [and others] -- Direct somatic embryogenesis in Coffea canephora / F. Quiroz-Figueroa [and others] -- A new temporary immersion bioreactor system for micropropagation / M.L. Robert [and others] -- Protocol to achieve photoautotrophic coconut plants cultured in vitro with improved performance ex vitro / G. Fuentes [and others] -- Use of statistics in plant biotechnology / M.E. Compton -- An efficient method for the micropropagation of Agave species / M.L. Robert [and others] -- Micropropagation of endangered plant species / Z. Liao [and others] -- Clonal propagation of softwoods / T.A. Thorpe, I.S. Harry and E.C. Yeung -- Isolation, culture, and plant regeneration from leaf protoplasts of Passiflora / M.R. Davey [and others] -- Isolation, culture, and plant regeneration from Echinacea purpurea protoplasts / Z.G. Pan [and others] -- Production of cybrids in Brassicaceae species / M. Vasylenko [and others] -- Guard cell protoplasts: isolation, culture, and regeneration of plants / G. Tallman -- Production of interspecific hybrid plants in Primula / J. Kato and M. Mii -- Agrobacterium-mediated transformation of Petunia leaf discs / I.M. van der Meer -- Transformation of wheat via particle bombardment / I.K. Vasil and V. Vasil -- Chloroplast transformation / X.M. Lu, W.B. Yin and Z.M. Hu -- The biochemical basis for the resistance to aluminum and their potential as selection markers / S.B. De los and T. Hernandez-Sotomayor -- Transformation of maize via Agrobacterium tumefaciens using a binary co-integrate vector system / Z.Y. Zhao and J. Ranch -- Capsaicin accumulation in Capsicum spp. suspension cultures / N. Ochoa-Alejo -- Isolation and purification of ribosome-inactivating proteins / S.W. Park [and others] -- Catharanthus roseus shoot cultures for the production of monoterpenoid indole alkaloids / E. Hernandez-Dominguez [and others] -- Methods for regeneration and transformation in Eschscholzia californica: A model plant to investigate alkaloid biosynthesis / B.P. MacLeod and P.J. Facchini -- The components of the culture media / V.M. Loyola-Vargas -- Plant biotechnology and tissue culture resources on the internet / V.M. Loyola-Vargas.

Print version record.

Following in the footsteps of its much-acclaimed first edition, Plant Cell Culture Protocols, Second Edition has been expanded and revised to include the most up-to-date and frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Two new appendices have also been added to explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, as well as to list more than 100 very useful internet sites. Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications.

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