PRINS and in situ PCR protocols.

Contributor(s): Pellestor, Franck
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 334.Publisher: Totowa, N.J. : Humana Press, ©2006Edition: 2nd ed. / edited by Franck PellestorDescription: 1 online resource (243 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 1588295494; 9781588295491; 9781597450683; 1597450685Subject(s): In situ hybridization -- Laboratory manuals | Polymerase chain reaction -- Laboratory manuals | Primed In Situ Labeling | Polymerase Chain Reaction -- methods | Polymerase Chain Reaction | Methods | Situ Hybridization | Investigative Techniques | Publication Formats | Nucleic Acid Amplification Techniques | Nucleic Acid Hybridization | Genetic Techniques | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Publication Characteristics | Staining and Labeling | Histocytological Preparation Techniques | Cytological Techniques | Histological Techniques | Clinical Laboratory Techniques | SCIENCE -- Life Sciences -- Molecular Biology | In situ hybridization | Polymerase chain reactionGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: PRINS and in situ PCR protocols.DDC classification: 572.8/4 LOC classification: QH452.8 | .P75 2006Online resources: Click here to access online
Contents:
New rapid multicolor PRINS protocol / J. Yan [and others] -- PRINS combined with peptide nucleic acid labeling / F. Pellestor and P. Paulasova -- PRINS combined with indirect immunofluorescence / P. Coullin -- PRINS for the detection of unique sequences / S.S. Wachtel and A.T. Tharapel -- Single-copy and point mutation analysis (rolling-circle PRINS) / J. Koch -- Analysis of sperm aneuploidy by PRINS / F. Pellestor [and others] -- In situ PCR protocols / O. Bagasra and T. Harris -- Detection and sizing of telomeric and other simple repeats by dideoxy-PRINS / J. Koch -- PRINS evaluation of chromosome instability in mammalian cells by detection of repetitive DNA sequences in micronuclei / A. Russo -- PRINS for the detection of gene deletions in cancer / A.T. Tharapel and S.S. Wachtel -- Combined fluorescence in situ hybridization and PRINS for the analysis of the Dystrophin gene / L. Stuppia, D. La Sala and C. Cinti -- Brain tissue preparations for chromosomal PRINS labeling / I.Y. Iourov [and others] -- PRINS on plant chromosomes / J. Macas [and others] -- Dual-color PRINS for in situ detection of fetal cells in maternal blood / K. Krabchi [and others] -- PRINS as an efficient tool for aneuploidy assessment in human oocytes and preimplantation embryos / F. Pellestor [and others] -- Microdissection with PCR in situ / A.T. Christian and C. Hara -- Application of reverse transcription in situ PCR in cancer analysis / T. Takeuchi [and others] -- In situ reverse transcription PCR on plant tissues / Z. Przybecki [and others] -- In situ PCR for the detection of human cytomegalovirus in suspension cells during the latent phase of infection / B. Slobedman -- Development and adaptation of the PRINS technology: an overview / F. Pellestor -- Latest developments in in situ PCR / O. Bagasra and T. Harris.
Summary: Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology.
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Includes bibliographical references and index.

New rapid multicolor PRINS protocol / J. Yan [and others] -- PRINS combined with peptide nucleic acid labeling / F. Pellestor and P. Paulasova -- PRINS combined with indirect immunofluorescence / P. Coullin -- PRINS for the detection of unique sequences / S.S. Wachtel and A.T. Tharapel -- Single-copy and point mutation analysis (rolling-circle PRINS) / J. Koch -- Analysis of sperm aneuploidy by PRINS / F. Pellestor [and others] -- In situ PCR protocols / O. Bagasra and T. Harris -- Detection and sizing of telomeric and other simple repeats by dideoxy-PRINS / J. Koch -- PRINS evaluation of chromosome instability in mammalian cells by detection of repetitive DNA sequences in micronuclei / A. Russo -- PRINS for the detection of gene deletions in cancer / A.T. Tharapel and S.S. Wachtel -- Combined fluorescence in situ hybridization and PRINS for the analysis of the Dystrophin gene / L. Stuppia, D. La Sala and C. Cinti -- Brain tissue preparations for chromosomal PRINS labeling / I.Y. Iourov [and others] -- PRINS on plant chromosomes / J. Macas [and others] -- Dual-color PRINS for in situ detection of fetal cells in maternal blood / K. Krabchi [and others] -- PRINS as an efficient tool for aneuploidy assessment in human oocytes and preimplantation embryos / F. Pellestor [and others] -- Microdissection with PCR in situ / A.T. Christian and C. Hara -- Application of reverse transcription in situ PCR in cancer analysis / T. Takeuchi [and others] -- In situ reverse transcription PCR on plant tissues / Z. Przybecki [and others] -- In situ PCR for the detection of human cytomegalovirus in suspension cells during the latent phase of infection / B. Slobedman -- Development and adaptation of the PRINS technology: an overview / F. Pellestor -- Latest developments in in situ PCR / O. Bagasra and T. Harris.

Print version record.

Carrying on the high standards of the much-praised first edition, leading researchers and clinical investigators have completely revised and updated PRINS and In Situ PCR Protocols to take advantage of the many new developments and applications that have occurred for cellular and molecular investigations. The authors contribute proven protocols for many variations of PRINS and in situ PCR, including a new, fast multicolor PRINS method for identifying human chromosomes, PRINS protocols for in situ detection of unique sequences and point mutations, a new dideoxy PRINS procedure, and in situ PCR and PRINS protocols for plants. They describe, in detail, various applications of PRINS in human cells (detection of gene deletions in cancer, detection of fetal cells in maternal blood, and assessment of aneuploidy in brain tissues and embryos) as well as in plant cells. Readily reproducible in situ PCR techniques are also presented for the detection of cytomegalovirus and for use in combination with microdissection. In situ RT-PCR techniques are also given for use in plant or cancer investigation. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, PRINS and In Situ PCR Protocols offers molecular biologists, cytogeneticists, and cytologists a cutting-edge guide to today's new in situ labeling technology.

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