MicroRNA protocols / edited by Shao-Yao Ying.

Contributor(s): Ying, Shao-Yao
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 342.Publisher: Totowa, N.J. : Humana Press, ©2006Description: 1 online resource (xiii, 365 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 1588295818; 1597451231; 9781597451239; 9781588295811; 1280832282; 9781280832284Subject(s): Small interfering RNA | MicroRNAs | RNA, Antisense | RNA, Small Untranslated | RNA | Antisense Elements (Genetics) | RNA, Untranslated | Nucleic Acids, Nucleotides, and Nucleosides | Nucleic Acids | Chemicals and Drugs | SCIENCE -- Life Sciences -- Molecular Biology | Small interfering RNAGenre/Form: Fulltext. | Illustration. | Internet Resource. Additional physical formats: Print version:: MicroRNA protocols.DDC classification: 572.8/8 LOC classification: QP623.5.S63 | M53 2006Online resources: Click here to access online
Contents:
The microRNA: overview of the RNA gene that modulates gene functions / Shao-Yao [and others] -- Structure analysis of microRNA precursors / Jacek Krol and Wlodzimierz J. Krzyzosiak -- MicroRNA biogenesis: isolation and characterization of the microprocessor complex / Richard K. Gregory, Thimmaiah P. Chendrimada, and Ramin Shiekhatter -- Recognition and cleavange of primary microRNA transcripts / Yan Zeng and Bryan R. Cullen -- Mouse embryonic stem cells as a model genetic system to dissect and exploit the RNA interference machinery / Stefan A. Muljo and Chryssa Kanellopoulou -- MicroRNAs and messenger RNA turnover / Julia A. Chekanova and Dmitry A. Belostotsky -- Prediction of microRNA targets / Marc Rehmsmeier -- Prediction of human microRNA targets / Bino John, Chris Sander, and Debora S. Marks -- Complications in mammalian microRNA target prediction / Neil R. Smalheiser and Vetle I. Torvik -- miRBase: the microRNA sequence database / Sam Griffiths-Jones.
Methodologies for high-throughput expression profiling of microRNAs / Paz Einat -- In situ hybridization as a tool to study the role of microRNAs in plant development / Catherine Kidner and Marja Timmermans -- Usefulness of the luciferase reporter system to test in efficacy of siRNA / Fengfeng Zhuang and Yi-Hsin Liu -- Cloning microRNAs from mammalian tissues / Michael Z. Michael -- Methods for analyzing microRNA expression and function during hematopoietic lineage differentiation / Hyeyoung Min and Chang-Zheng Chen -- Identifying microRNNA regulators of cell death in Drosophila / Chun-Hong Chen, Ming Guo, and Bruce A. Hay -- MicroRNAs in human immunodeficiency virus-1 infection / Yamina Bennasser [and others] -- Cloning and detection of HIV-1-encoded microRNA / Shinya Omoto and Yoichi R. Fujii.
Identification of messenger RNAs and microRNAs associated with fragile X mental retardation protein / Ranhui Duan and Peng Jin -- In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates / Akira Ishizuka [and others] -- Downregulation of human Cdc6 protein using a lentivirus RNA interference expression vector / Feng Luo [and others] -- Gene silencing in vitro and in vivo using intronic microRNAs / Shi-Lung Lin, donald C. Chang, and Shao-Yao Ying -- Transgene-link animal models using intronic microRNAs / Shi-Lung Lin, Shin-Ju E. chang, and Shao-Yao Ying -- Evolution of microRNAs / Andrea Tanzer and Peter F. Stadler -- Perspectives / Shao-Yao Ying [and others].
Summary: It is now known that microRNA (miRNA), once thought to be junk, can suppress the expression of other genes and may be involved in numerous cellular processes, including cell proliferation, apoptosis, cell differentiation, and development. In MicroRNA Protocols, expert miRNA researchers explain its basic concepts and introduce the most advanced technologies and techniques for predicting screening, isolating, and assaying it. The authors provide diverse, novel, and useful descriptions of miRNAs in several species-including plants, worms, flies, fish, chicks, mice, and humans-and show how they have been employed to develop miRNA as a potential drug design tool. Highlights include miRNA and their target prediction by computer analysis, functional assays of miRNA in vitro and in vivo, and transgenic animal models using miRNA technologies. Additional chapters cover gene regulation, small RNA function, RNA interference mechanisms, and transgenetics. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Eminently practical and cutting-edge, MicroRNA Protocols affords biomedical researchers readily reproducible techniques to understand and study the molecular pathogenesis of disease and design new therapeutic strategies.
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Includes bibliographical references and index.

The microRNA: overview of the RNA gene that modulates gene functions / Shao-Yao [and others] -- Structure analysis of microRNA precursors / Jacek Krol and Wlodzimierz J. Krzyzosiak -- MicroRNA biogenesis: isolation and characterization of the microprocessor complex / Richard K. Gregory, Thimmaiah P. Chendrimada, and Ramin Shiekhatter -- Recognition and cleavange of primary microRNA transcripts / Yan Zeng and Bryan R. Cullen -- Mouse embryonic stem cells as a model genetic system to dissect and exploit the RNA interference machinery / Stefan A. Muljo and Chryssa Kanellopoulou -- MicroRNAs and messenger RNA turnover / Julia A. Chekanova and Dmitry A. Belostotsky -- Prediction of microRNA targets / Marc Rehmsmeier -- Prediction of human microRNA targets / Bino John, Chris Sander, and Debora S. Marks -- Complications in mammalian microRNA target prediction / Neil R. Smalheiser and Vetle I. Torvik -- miRBase: the microRNA sequence database / Sam Griffiths-Jones.

Methodologies for high-throughput expression profiling of microRNAs / Paz Einat -- In situ hybridization as a tool to study the role of microRNAs in plant development / Catherine Kidner and Marja Timmermans -- Usefulness of the luciferase reporter system to test in efficacy of siRNA / Fengfeng Zhuang and Yi-Hsin Liu -- Cloning microRNAs from mammalian tissues / Michael Z. Michael -- Methods for analyzing microRNA expression and function during hematopoietic lineage differentiation / Hyeyoung Min and Chang-Zheng Chen -- Identifying microRNNA regulators of cell death in Drosophila / Chun-Hong Chen, Ming Guo, and Bruce A. Hay -- MicroRNAs in human immunodeficiency virus-1 infection / Yamina Bennasser [and others] -- Cloning and detection of HIV-1-encoded microRNA / Shinya Omoto and Yoichi R. Fujii.

Identification of messenger RNAs and microRNAs associated with fragile X mental retardation protein / Ranhui Duan and Peng Jin -- In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates / Akira Ishizuka [and others] -- Downregulation of human Cdc6 protein using a lentivirus RNA interference expression vector / Feng Luo [and others] -- Gene silencing in vitro and in vivo using intronic microRNAs / Shi-Lung Lin, donald C. Chang, and Shao-Yao Ying -- Transgene-link animal models using intronic microRNAs / Shi-Lung Lin, Shin-Ju E. chang, and Shao-Yao Ying -- Evolution of microRNAs / Andrea Tanzer and Peter F. Stadler -- Perspectives / Shao-Yao Ying [and others].

Print version record.

It is now known that microRNA (miRNA), once thought to be junk, can suppress the expression of other genes and may be involved in numerous cellular processes, including cell proliferation, apoptosis, cell differentiation, and development. In MicroRNA Protocols, expert miRNA researchers explain its basic concepts and introduce the most advanced technologies and techniques for predicting screening, isolating, and assaying it. The authors provide diverse, novel, and useful descriptions of miRNAs in several species-including plants, worms, flies, fish, chicks, mice, and humans-and show how they have been employed to develop miRNA as a potential drug design tool. Highlights include miRNA and their target prediction by computer analysis, functional assays of miRNA in vitro and in vivo, and transgenic animal models using miRNA technologies. Additional chapters cover gene regulation, small RNA function, RNA interference mechanisms, and transgenetics. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Eminently practical and cutting-edge, MicroRNA Protocols affords biomedical researchers readily reproducible techniques to understand and study the molecular pathogenesis of disease and design new therapeutic strategies.

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