Mesenchymal stem cells : methods and protocols / edited by Darwin J. Prockop, Donald G. Phinney, Bruce A. Bunnell.

Contributor(s): Prockop, Darwin J | Phinney, Donald G | Bunnell, Bruce A
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 449.Publisher: Totowa, N.J. : Humana Press, ©2008Description: 1 online resource (xvi, 192 pages) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781603271691; 1603271694Subject(s): Stem cells -- Laboratory manuals | Mesenchymal Stem Cells | Cytological Techniques | Stem cells | Cytology | Biology | Health & Biological Sciences | stamcellen | stem cells | beenmergcellen | bone marrow cells | celdifferentiatie | cell differentiation | Cellular Biology | CelbiologieGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Mesenchymal stem cells.DDC classification: 616/.02774 LOC classification: QH588.S83 | M47 2008Online resources: Click here to access online
Contents:
pt. I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe [and others] ; Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger ; A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C.W. Zannettino ; Adipose-derived stem cells / John K. Fraser [and others] ; Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois [and others] -- pt. II. Manipulation of human multipotential stromal cells ; Colony forming unit assays for MSCs / Radhika Pochampally ; Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe ; Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe ; Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi ; Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prockop ; Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- pt. III. Isolation and characterization of murine multipotential stromal cells from bone marrow ; Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney.
In: Springer ProtocolsSummary: For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In Mesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study. Written to the specifications of the highly successful Methods in Molecular Biology series format, each chapter contains step-by-step laboratory protocols, a list of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls. Cutting edge and easy to use, Mesenchymal Stem Cells: Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.
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Includes bibliographical references and index.

pt. I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe [and others] ; Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger ; A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C.W. Zannettino ; Adipose-derived stem cells / John K. Fraser [and others] ; Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois [and others] -- pt. II. Manipulation of human multipotential stromal cells ; Colony forming unit assays for MSCs / Radhika Pochampally ; Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe ; Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe ; Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi ; Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prockop ; Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- pt. III. Isolation and characterization of murine multipotential stromal cells from bone marrow ; Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney.

Print version record.

For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In Mesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study. Written to the specifications of the highly successful Methods in Molecular Biology series format, each chapter contains step-by-step laboratory protocols, a list of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls. Cutting edge and easy to use, Mesenchymal Stem Cells: Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.

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