Herpes simplex virus protocols / edited by S. Moira Brown and Alasdair R. MacLean.

Contributor(s): Brown, S. Moira | MacLean, Alasdair R
Material type: TextTextSeries: Methods in molecular medicine: 10.Publisher: Totowa, N.J. : Humana Press, ©1998Description: 1 online resource (xi, 419 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592595945; 1592595944Subject(s): Herpes simplex virus -- Laboratory manuals | Herpes Simplex -- diagnosis | Simplexvirus -- physiology | Herpes simplex virus | Herpes simplex virus | Wetenschappelijke technieken | Aufsatzsammlung | Herpes-simplex-Virus | Microbiology & Immunology | Biology | Health & Biological SciencesGenre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Herpes simplex virus protocols.DDC classification: 616.5/22 | 616.9/101 LOC classification: QR400.2.H47 | H47 1998Other classification: 44.43 | XD 7400 Online resources: Click here to access online
Contents:
HSV growth, preparation, and assay / J. Harland and S.M. Brown-HSV entry and spread / C.A. Maclean-Preparation of HSV-DNA and production of infectious virus / A.R. Maclean-HSV mutagenesis / R.S. Coffin-Saturating mutagenesis and characterization of a herpesvirus genome using in vivo reconstitution of virus from cloned subgenomic regions / N. de Wind, M. van Zijl and A. Berns-Construction and use of cell lines expressing HSV genes / C. Entwisle-Analysis of HSV polypeptides / L. Haarr and N. Langeland-Protein purification / J. Conner-Expression and purification of secreted forms of HSV glycoproteins from baculovirus-infected insect cells / S.H. Willis [and others]-Crystallization of macromolecules for three-dimensional structure determination / B. Luisi, M. Anderson and G. Hope-Direct immunogold labeling of herpesvirus suspensions / L.M. Stannard-Expression of HSV proteins in bacteria / E.A. McKie-In vitro systems to analyze HSV transcript processing / A. Phelan and J.B. Clements-Analysis of HSV-1 transcripts by RNA-pcr / J.G. Spivack-Transient assays for HSV origin and replication protein function / N.D. Stow-HSV amplicons in gene therapy / N. Frenkel and R. Sarid-Analyses of HSV proteins for posttranslational modifications and enzyme functions / J.A. Blaho and B. Roizman-HSV-cellular protein interactions / D.S. Latchman-Models of recurrent infection with HSV in the skin and eye of the mouse / T.J. Hill and C. Shimeld-Pathogenesis and molecular biology of HSV latency and ocular reactivation in the rabbit / J.M. Hill, R. Wen and W.P. Halford-HSV latency in vitro : In situ hybridization methods / C.L. Wilcox and R.L. Smith-Assessing cell-mediated immune responses to HSV in murine systems / T.A. Banks, M.J. Hariharan and B.T. Rouse-Assays for HSV gene expression during establishment and maintenance of latent infection / P. Speck and S. Efstathiou-Analysis of HSV-DNA and RNA using the polymerase chain reaction / R. Ramakrishnan, D.J. Fink and M. Levine-HSV vectors for gene therapy / D.C. Bloom-Investigation of the anti-HSV activity of candidate antiviral agents / D.J. Dargan.
Action note: digitized 2010 committed to preserve In: Springer ProtocolsSummary: An outstanding collection of state-of-the-art experimental methods for herpes virology. These procedures range from the more biological in vivo maneuvers to the purely molecular, and are described in detail by selected experts to ensure reproducibility. Most of the protocols rely on the herpes simplex virus as prototype, so that it then becomes relatively easy to extrapolate and make the necessary modifications required for application to other herpesviruses, especially members of the alpha group, such as PRV and EHV.
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Includes bibliographical references and index.

HSV growth, preparation, and assay / J. Harland and S.M. Brown-HSV entry and spread / C.A. Maclean-Preparation of HSV-DNA and production of infectious virus / A.R. Maclean-HSV mutagenesis / R.S. Coffin-Saturating mutagenesis and characterization of a herpesvirus genome using in vivo reconstitution of virus from cloned subgenomic regions / N. de Wind, M. van Zijl and A. Berns-Construction and use of cell lines expressing HSV genes / C. Entwisle-Analysis of HSV polypeptides / L. Haarr and N. Langeland-Protein purification / J. Conner-Expression and purification of secreted forms of HSV glycoproteins from baculovirus-infected insect cells / S.H. Willis [and others]-Crystallization of macromolecules for three-dimensional structure determination / B. Luisi, M. Anderson and G. Hope-Direct immunogold labeling of herpesvirus suspensions / L.M. Stannard-Expression of HSV proteins in bacteria / E.A. McKie-In vitro systems to analyze HSV transcript processing / A. Phelan and J.B. Clements-Analysis of HSV-1 transcripts by RNA-pcr / J.G. Spivack-Transient assays for HSV origin and replication protein function / N.D. Stow-HSV amplicons in gene therapy / N. Frenkel and R. Sarid-Analyses of HSV proteins for posttranslational modifications and enzyme functions / J.A. Blaho and B. Roizman-HSV-cellular protein interactions / D.S. Latchman-Models of recurrent infection with HSV in the skin and eye of the mouse / T.J. Hill and C. Shimeld-Pathogenesis and molecular biology of HSV latency and ocular reactivation in the rabbit / J.M. Hill, R. Wen and W.P. Halford-HSV latency in vitro : In situ hybridization methods / C.L. Wilcox and R.L. Smith-Assessing cell-mediated immune responses to HSV in murine systems / T.A. Banks, M.J. Hariharan and B.T. Rouse-Assays for HSV gene expression during establishment and maintenance of latent infection / P. Speck and S. Efstathiou-Analysis of HSV-DNA and RNA using the polymerase chain reaction / R. Ramakrishnan, D.J. Fink and M. Levine-HSV vectors for gene therapy / D.C. Bloom-Investigation of the anti-HSV activity of candidate antiviral agents / D.J. Dargan.

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An outstanding collection of state-of-the-art experimental methods for herpes virology. These procedures range from the more biological in vivo maneuvers to the purely molecular, and are described in detail by selected experts to ensure reproducibility. Most of the protocols rely on the herpes simplex virus as prototype, so that it then becomes relatively easy to extrapolate and make the necessary modifications required for application to other herpesviruses, especially members of the alpha group, such as PRV and EHV.

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