DNA sequencing protocols / edited by Hugh G. Griffin and Annette M. Griffin.

Contributor(s): Griffin, Hugh G | Griffin, Annette M
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 23.Publisher: Totowa, N.J. : Humana Press, ©1993Description: 1 online resource (xii, 392 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592595105; 1592595103; 9781489939968; 1489939962Subject(s): Nucleotide sequence | Sequence Analysis, DNA | Nucleotide sequenceGenre/Form: Electronic books. Additional physical formats: Print version:: DNA sequencing protocols.DDC classification: 574.87/3282 LOC classification: QP624 | .D634 1993Online resources: Click here to access online
Contents:
DNA sequencing / Hugh G. Griffin and Annette M. Griffin -- M13 cloning vehicles : their contribution to DNA sequencing / Joachim Messing -- Cloning into M13 / Quinzhong Yu -- Transfection of E. coli with M13 DNA / Fiona M. Tomley -- M13 phage growth and single-strand DNA preparation / Fiona M. Tomley -- M13 phage growth and DNA purification using 96 well microtiter trays / Alan T. Bankier -- Generation of random fragments by sonication / Alan T. Bankier -- Generation of a nested set of deletions using exonuclease III / George Murphy -- Sequential deletions of single-stranded DNA : cyclone sequencing / George Murphy -- Subcloning for DNA sequencing / Gary M. Studnicka [and others] -- Sequencing using custom designed oligonucleotides / Ulrike Gerischer and Peter Durre -- Dideoxy sequencing reactions using Klenow fragment DNA polymerase I / Alan T. Bankier -- Dideoxy sequencing reactions using T7 polymerase / Peter Dürre and Ulrike Gerischer -- Dideoxy sequencing reactions using sequenase version 2.0 / Hugh G. Griffin and Annette M. Griffin -- Dideoxy sequencing reactions using Taq polymerase / Fabrizio Arigoni and P. Alexandre Kaminski -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Plasmid sequencing / Hugh G. Griffin and Annette M. Griffin -- Plasmid sequencing / George Murphy -- Direct sequencing of inserts cloned into lambda vectors / Afshan N. Malik -- Cosmid sequencing / Molly Craxton -- Genomic sequencing / Gerd P. Pfeifer and Arthur D. Riggs -- Sequencing of double-stranded PCR products / Susannah Gal -- Sequencing double-stranded linear DNA with sequenase and [[alpha]-³⁵S] dATP / Jean-Laurent Casanova -- Solid phase PCR sequencing of biotinylated products / Andrew Green and Mark Vaudin -- Cycle sequencing / Robert W. Blakesley -- Direct blotting electrophoresis / Stephan Beck -- Multiplex DNA sequencing / Stephan Beck -- DNA sequencing by chemiluminescent detection / Stephan Beck -- Reverse sequencing of M13 cloned DNA / Alan T. Bankier -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- DNA sequencing by chemical degradation using one, two, and four different fluorophores / André Rosenthal -- Linear amplification sequencing with dye terminators / André Rosenthal and D. Stephen Charnock-Jones -- Sequencing reactions for the Applied Biosystems 373A automated DNA sequencer / Nicolette Halloran, Zijin Du and Richard K. Wilson -- Sequencing reactions for ALF (EMBL) automated DNA sequencer / Wilhelm Ansorge [and others] -- Sequencing using the DuPont Genesis 2000 DNA analysis system / Len Hall -- Use of robotic workstations in DNA sequencing / Alan T. Bankier.
Summary: DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting electrophoresis, sequencing of chemiluminescence, and automated sequencing.
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Includes bibliographical references and index.

DNA sequencing / Hugh G. Griffin and Annette M. Griffin -- M13 cloning vehicles : their contribution to DNA sequencing / Joachim Messing -- Cloning into M13 / Quinzhong Yu -- Transfection of E. coli with M13 DNA / Fiona M. Tomley -- M13 phage growth and single-strand DNA preparation / Fiona M. Tomley -- M13 phage growth and DNA purification using 96 well microtiter trays / Alan T. Bankier -- Generation of random fragments by sonication / Alan T. Bankier -- Generation of a nested set of deletions using exonuclease III / George Murphy -- Sequential deletions of single-stranded DNA : cyclone sequencing / George Murphy -- Subcloning for DNA sequencing / Gary M. Studnicka [and others] -- Sequencing using custom designed oligonucleotides / Ulrike Gerischer and Peter Durre -- Dideoxy sequencing reactions using Klenow fragment DNA polymerase I / Alan T. Bankier -- Dideoxy sequencing reactions using T7 polymerase / Peter Dürre and Ulrike Gerischer -- Dideoxy sequencing reactions using sequenase version 2.0 / Hugh G. Griffin and Annette M. Griffin -- Dideoxy sequencing reactions using Taq polymerase / Fabrizio Arigoni and P. Alexandre Kaminski -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Plasmid sequencing / Hugh G. Griffin and Annette M. Griffin -- Plasmid sequencing / George Murphy -- Direct sequencing of inserts cloned into lambda vectors / Afshan N. Malik -- Cosmid sequencing / Molly Craxton -- Genomic sequencing / Gerd P. Pfeifer and Arthur D. Riggs -- Sequencing of double-stranded PCR products / Susannah Gal -- Sequencing double-stranded linear DNA with sequenase and [[alpha]-³⁵S] dATP / Jean-Laurent Casanova -- Solid phase PCR sequencing of biotinylated products / Andrew Green and Mark Vaudin -- Cycle sequencing / Robert W. Blakesley -- Direct blotting electrophoresis / Stephan Beck -- Multiplex DNA sequencing / Stephan Beck -- DNA sequencing by chemiluminescent detection / Stephan Beck -- Reverse sequencing of M13 cloned DNA / Alan T. Bankier -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- DNA sequencing by chemical degradation using one, two, and four different fluorophores / André Rosenthal -- Linear amplification sequencing with dye terminators / André Rosenthal and D. Stephen Charnock-Jones -- Sequencing reactions for the Applied Biosystems 373A automated DNA sequencer / Nicolette Halloran, Zijin Du and Richard K. Wilson -- Sequencing reactions for ALF (EMBL) automated DNA sequencer / Wilhelm Ansorge [and others] -- Sequencing using the DuPont Genesis 2000 DNA analysis system / Len Hall -- Use of robotic workstations in DNA sequencing / Alan T. Bankier.

Print version record.

DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting electrophoresis, sequencing of chemiluminescence, and automated sequencing.

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