Neuroprotection methods and protocols / edited by Tiziana Borsello.

Contributor(s): Borsello, Tiziana
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 399.Publisher: Totowa, N.J. : Humana Press, ©2007Description: 1 online resource (xii, 239 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781597455046; 1597455040Subject(s): Nervous system -- Degeneration | Nervous system -- Diseases | Cellular signal transduction | Neurodegenerative Diseases -- prevention & control | Biomedical Research -- methods | Cytoprotection | Neurodegenerative Diseases -- physiopathology | Neuroprotective Agents | Nervous system -- Degeneration | Nervous system -- Diseases | Cellular signal transduction | Cellular signal transduction | Nervous system -- Degeneration | Nervous system -- Diseases | neurologie | neurology | zenuwstelselziekten | nervous system diseases | moleculaire biologie | molecular biology | biologische technieken | biological techniques | neurobiologie | neurobiology | protocollen | protocols | Cellular Biology | Molecular Biology (General) | Celbiologie | Moleculaire biologie (algemeen)Genre/Form: Electronic books. Additional physical formats: Print version:: Neuroprotection methods and protocols.DDC classification: 616.8047 LOC classification: RC365Other classification: R741. 02 Online resources: Click here to access online
Contents:
Neuronal death and neuroprotection : a review / Mariaelena Repici, Jean Mariani, and Tiziana Borsello -- The use of propidium iodide to assess excitotoxic neuronal death in primary mixed cortical cultures / Anthony C. Lau, Hong Cui, and Michael Tymianski -- A modified silver technique (de Olmos Stain) for assessment of neuronal and axonal degeneration / Tatyana I. Tenkova and Mark P. Goldberg -- Model of acute injury to study neuroprotection / Michal Schwartz and Jonathan Kipnis -- Organotypic entorhino-hippocampal slice cultures : a tool to study the molecular and cellular regulation of axonal regeneration and collateral sprouting in vitro / Domenico Del Turco and Thomas Deller -- Role of Nrf2-dependent ARE-driven antioxidant pathway in neuroprotection / Jiang Li [and others] -- Biochemical methods to assess the coupling of brain energy metabolism in control and disease states / Jennifer Zechel, W. David Lust, and Michele Puchowicz -- A method for isolating prosurvival targets of NF-[kappa]B/rel transcription factors / Christian Kuntzen [and others] -- Functional cloning of genes regulating apoptosis in neuronal cells / Roberta Visconti and Luciano D'Adamio -- Characterization of mRNA expression in single neurons / David M. Lin [and others] -- Brain on a chip : a method to detect novel neuroprotective candidate targets / Yang Tang and Myriam Bernaudin -- Intracerebral infusion of neurotrophic factors / Theo Hagg -- Synthesis of cell-penetrating peptides and their application in neurobiology / Gunnar P.H. Dietz and Mathias Bähr -- The stem cells as a potential treatment for neurodegeneration / Ferrari Daniela, Angelo Luigi Vescovi, and Daniele Bottai -- In vivo tomographic imaging studies of neurodegeneration and neuroprotection : a review / Rikki N. Waterhouse and Jun Zhao.
Summary: Due to an aging population, the incidence of age-related neurodegenerative diseases is expected to rise dramatically. In Neuroprotection Methods and Protocols, a team of skilled, renowned researchers provide step-by-step laboratory protocols intended to slow or prevent the progression of such diseases. Focusing on neuroprotective approaches, this volume describes basic scientific discoveries and the application of such research within different laboratories in a clear and systematic way in order to orient scientists in this complicated field. The chapters follow the Methods in Molecular Biology series format, consisting of readily reproducible methods, lists of the necessary materials, and a Notes section which aids the researcher in solving and avoiding technical problems. Comprehensive and easy to use, Neuroprotection Methods and Protocols is the perfect reference for basic neuroscientists hoping to move forward the ever-important field of neuroprotection and neuronal death research.
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Includes bibliographical references and index.

Neuronal death and neuroprotection : a review / Mariaelena Repici, Jean Mariani, and Tiziana Borsello -- The use of propidium iodide to assess excitotoxic neuronal death in primary mixed cortical cultures / Anthony C. Lau, Hong Cui, and Michael Tymianski -- A modified silver technique (de Olmos Stain) for assessment of neuronal and axonal degeneration / Tatyana I. Tenkova and Mark P. Goldberg -- Model of acute injury to study neuroprotection / Michal Schwartz and Jonathan Kipnis -- Organotypic entorhino-hippocampal slice cultures : a tool to study the molecular and cellular regulation of axonal regeneration and collateral sprouting in vitro / Domenico Del Turco and Thomas Deller -- Role of Nrf2-dependent ARE-driven antioxidant pathway in neuroprotection / Jiang Li [and others] -- Biochemical methods to assess the coupling of brain energy metabolism in control and disease states / Jennifer Zechel, W. David Lust, and Michele Puchowicz -- A method for isolating prosurvival targets of NF-[kappa]B/rel transcription factors / Christian Kuntzen [and others] -- Functional cloning of genes regulating apoptosis in neuronal cells / Roberta Visconti and Luciano D'Adamio -- Characterization of mRNA expression in single neurons / David M. Lin [and others] -- Brain on a chip : a method to detect novel neuroprotective candidate targets / Yang Tang and Myriam Bernaudin -- Intracerebral infusion of neurotrophic factors / Theo Hagg -- Synthesis of cell-penetrating peptides and their application in neurobiology / Gunnar P.H. Dietz and Mathias Bähr -- The stem cells as a potential treatment for neurodegeneration / Ferrari Daniela, Angelo Luigi Vescovi, and Daniele Bottai -- In vivo tomographic imaging studies of neurodegeneration and neuroprotection : a review / Rikki N. Waterhouse and Jun Zhao.

Print version record.

Due to an aging population, the incidence of age-related neurodegenerative diseases is expected to rise dramatically. In Neuroprotection Methods and Protocols, a team of skilled, renowned researchers provide step-by-step laboratory protocols intended to slow or prevent the progression of such diseases. Focusing on neuroprotective approaches, this volume describes basic scientific discoveries and the application of such research within different laboratories in a clear and systematic way in order to orient scientists in this complicated field. The chapters follow the Methods in Molecular Biology series format, consisting of readily reproducible methods, lists of the necessary materials, and a Notes section which aids the researcher in solving and avoiding technical problems. Comprehensive and easy to use, Neuroprotection Methods and Protocols is the perfect reference for basic neuroscientists hoping to move forward the ever-important field of neuroprotection and neuronal death research.

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