PCR cloning protocols : from molecular cloning to genetic engineering / edited by Bruce A. White.

Contributor(s): White, Bruce Alan
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 67.Publisher: Totowa, N.J. : Humana Press, ©1997Description: 1 online resource (xiv, 490 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592595532; 1592595537; 0896034836; 9780896034839Subject(s): Molecular cloning -- Laboratory manuals | Polymerase chain reaction -- Laboratory manuals | Cloning, Molecular | Polymerase Chain Reaction | Molecular cloning | Polymerase chain reaction | polymerase chain reaction | dna cloning | genetische modificatie | genetic engineering | biologische technieken | biological techniques | protocollen | protocols | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: PCR cloning protocols : from molecular cloning to genetic engineering.DDC classification: 574.87/3282 LOC classification: QH442.2 | .P37 1997Online resources: Click here to access online
Contents:
PCR : basic principles and routine practice / Lori A. Kolmodin, J. Fenton Williams -- XL PCR amplification of long targets from genomic DNA / Suzanne Cheng, Lori A. Kolmodin -- Amplification of DNA sequences up to 5 kb from small amounts of genomic DNA using tub DNA polymerase / Helen B. Forrester, Ian R. Radford -- One-step optimization using touchdown and stepdown PCR / Kenneth H. Roux, Karl H. Hecker -- GC-rich template amplification by inverse PCR : DNA polymerase and solvent effects / Alain Moreau, Colette Duez, Jean Dusart -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments / Jyrki T. Aatsinki -- Using T4 DNA polymerase to generate clonable PCR products / Kai Wang -- Rapid (ligase-free) subcloning of PCR products / Alan R. Shuldiner, Keith Tanner -- Cloning PCR products utilizing the T/A overhang and a kit / Melissa Lail-Trecker -- Cloning unmodified PCR products using engineered Xcml restriction sites in a portable cassette / Alessandro Testori, Paul Sollitti -- A T-linker strategy for modification and directional cloning of PCR products / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Recovery of DNA amplification products from silver-stained polyacrylamide gels : applications in nucleic acid fingerprinting and genetic mapping / Gustavo Caetano-Anollés, Robert N. Trigiano -- Recombination and site-directed mutagenesis using recombination PCR / Douglas H. Jones, Stanley C. Winistorfer -- In vitro recombination and mutagenesis of DNA : SOEing together tailor-made genes / Robert M. Horton -- In-frame cloning of synthetic genes using PCR inserts / James C. Pierce -- Creation of chimeric junctions, deletions, and insertions by PCR / Geneviève Pont-Kingdon -- Mutagenesis and gene fusion by megaprimer PCR / Sailen Barik -- Rapid and efficient one-tube PCR-based mutagenesis method / Veronique Picard, Susan Clark Bock -- Thermostable ligase-mediated incorporation of mutagenic oligonucleotides during PCR amplification / Scott F. Michael -- Linker scanning mutagenesis by three-step PCR / Judith T. Schanke -- Sequence inversion by flip-PCR / Judith T. Schanke -- PCR site-directed mutagenesis using pyrococcus sp GB-D polymerase coupled to a rapid screening procedure : application to a [beta]-glucanase gene / Jaume Pons, Antoni Planas, Miquel Juncosa, Enrique Querol -- Using the SELEX combinatorial chemistry process to find high affinity nucleic acid ligands to target molecules / Craig Tuerk -- Rapid amplification of cDNA ends / David Bertioli -- Amplification of gene-regulating regions with single-sided specificity / Mei-Zhong Luo, Rino Cella -- An end-trimming method and its application to amplify adjacent cDNA and genomic DNA fragments by PCR / Hiroyuki Iwahana, Mitsuo Itakura -- Anchoring a defined sequence to the 5' ends of mRNAs : the bolt to clone rare full-length mRNAs / Jean Baptiste Edwards, Olivier Valdenaire, Jacques Mallet -- Rapid directional walk within DNA clones by step-out PCR / Umadevi V. Wesley, Cedric S. Wesley -- Inverse PCR : an efficient approach to cloning cDNA ends / Sheng-He Huang -- Rapid amplification of gene ends (RAGE) from gene libraries by anchored PCR / Sheng-He Huang, Ambrose Y. Jong -- Isolation of coding sequences from yeast artificial chromosome (Yac) : clones by exon amplification / Fernando Gibson, Steve D. Brown -- cDNA libraries from a low amount of cells / Philippe Ravassard, Christine Icard-Liepkalns, Jacques Mallet, Jean Baptiste Edwards -- Rapid and nonradioactive screening of recombinant libraries by PCR / Michael W. King -- Use of PCR for cDNA library screening / Toru Takumi -- Generation and PCR screening of bacteriophage [lambda] sublibraries enriched for rare clones (the "sublibrary method") / Michael Lardelli -- Normalization of cDNA sequence representation by molecular selection / Thierry G. Coche -- Subtractive cDNA cloning using magnetic beads and PCR / Thierry G. Coche -- Generation of a PCR-renewable source of subtractive cDNA / W. Michael Kuehl, James Battey -- The use of PCR for differential screening of cDNA libraries / Mark G. Thomas, Sarah A. Hesse, Yvonne J. Foss, Farzin Farzaneh -- Identification and cloning of differentially expressed genes by DDRT-PCR / Mikkel Rohde, Rene Hummel, Niels Pallisgaard, Tino Podstufka, Heidi Riedel, Henrik Leffers, Michael Strauss -- Cloning gene family members using PCR with degenerate oligonucleotide primers / Gregory M. Preston -- Amplification using degenerate primers with multiple inosines to isolate genes with minimal sequence similarity / Simona Bartl -- Designing PCR primers to amplify specific members or subgroups of multigene families / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Screening gene family-enriched cDNA sublibraries with an unamplified cDNA probe : focusing on moderately to abundantly expressed clones / Meimei Hu, Bruce A. White.
Summary: Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs.
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Includes bibliographical references and index.

PCR : basic principles and routine practice / Lori A. Kolmodin, J. Fenton Williams -- XL PCR amplification of long targets from genomic DNA / Suzanne Cheng, Lori A. Kolmodin -- Amplification of DNA sequences up to 5 kb from small amounts of genomic DNA using tub DNA polymerase / Helen B. Forrester, Ian R. Radford -- One-step optimization using touchdown and stepdown PCR / Kenneth H. Roux, Karl H. Hecker -- GC-rich template amplification by inverse PCR : DNA polymerase and solvent effects / Alain Moreau, Colette Duez, Jean Dusart -- Coupled one-step reverse transcription and polymerase chain reaction procedure for cloning large cDNA fragments / Jyrki T. Aatsinki -- Using T4 DNA polymerase to generate clonable PCR products / Kai Wang -- Rapid (ligase-free) subcloning of PCR products / Alan R. Shuldiner, Keith Tanner -- Cloning PCR products utilizing the T/A overhang and a kit / Melissa Lail-Trecker -- Cloning unmodified PCR products using engineered Xcml restriction sites in a portable cassette / Alessandro Testori, Paul Sollitti -- A T-linker strategy for modification and directional cloning of PCR products / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Recovery of DNA amplification products from silver-stained polyacrylamide gels : applications in nucleic acid fingerprinting and genetic mapping / Gustavo Caetano-Anollés, Robert N. Trigiano -- Recombination and site-directed mutagenesis using recombination PCR / Douglas H. Jones, Stanley C. Winistorfer -- In vitro recombination and mutagenesis of DNA : SOEing together tailor-made genes / Robert M. Horton -- In-frame cloning of synthetic genes using PCR inserts / James C. Pierce -- Creation of chimeric junctions, deletions, and insertions by PCR / Geneviève Pont-Kingdon -- Mutagenesis and gene fusion by megaprimer PCR / Sailen Barik -- Rapid and efficient one-tube PCR-based mutagenesis method / Veronique Picard, Susan Clark Bock -- Thermostable ligase-mediated incorporation of mutagenic oligonucleotides during PCR amplification / Scott F. Michael -- Linker scanning mutagenesis by three-step PCR / Judith T. Schanke -- Sequence inversion by flip-PCR / Judith T. Schanke -- PCR site-directed mutagenesis using pyrococcus sp GB-D polymerase coupled to a rapid screening procedure : application to a [beta]-glucanase gene / Jaume Pons, Antoni Planas, Miquel Juncosa, Enrique Querol -- Using the SELEX combinatorial chemistry process to find high affinity nucleic acid ligands to target molecules / Craig Tuerk -- Rapid amplification of cDNA ends / David Bertioli -- Amplification of gene-regulating regions with single-sided specificity / Mei-Zhong Luo, Rino Cella -- An end-trimming method and its application to amplify adjacent cDNA and genomic DNA fragments by PCR / Hiroyuki Iwahana, Mitsuo Itakura -- Anchoring a defined sequence to the 5' ends of mRNAs : the bolt to clone rare full-length mRNAs / Jean Baptiste Edwards, Olivier Valdenaire, Jacques Mallet -- Rapid directional walk within DNA clones by step-out PCR / Umadevi V. Wesley, Cedric S. Wesley -- Inverse PCR : an efficient approach to cloning cDNA ends / Sheng-He Huang -- Rapid amplification of gene ends (RAGE) from gene libraries by anchored PCR / Sheng-He Huang, Ambrose Y. Jong -- Isolation of coding sequences from yeast artificial chromosome (Yac) : clones by exon amplification / Fernando Gibson, Steve D. Brown -- cDNA libraries from a low amount of cells / Philippe Ravassard, Christine Icard-Liepkalns, Jacques Mallet, Jean Baptiste Edwards -- Rapid and nonradioactive screening of recombinant libraries by PCR / Michael W. King -- Use of PCR for cDNA library screening / Toru Takumi -- Generation and PCR screening of bacteriophage [lambda] sublibraries enriched for rare clones (the "sublibrary method") / Michael Lardelli -- Normalization of cDNA sequence representation by molecular selection / Thierry G. Coche -- Subtractive cDNA cloning using magnetic beads and PCR / Thierry G. Coche -- Generation of a PCR-renewable source of subtractive cDNA / W. Michael Kuehl, James Battey -- The use of PCR for differential screening of cDNA libraries / Mark G. Thomas, Sarah A. Hesse, Yvonne J. Foss, Farzin Farzaneh -- Identification and cloning of differentially expressed genes by DDRT-PCR / Mikkel Rohde, Rene Hummel, Niels Pallisgaard, Tino Podstufka, Heidi Riedel, Henrik Leffers, Michael Strauss -- Cloning gene family members using PCR with degenerate oligonucleotide primers / Gregory M. Preston -- Amplification using degenerate primers with multiple inosines to isolate genes with minimal sequence similarity / Simona Bartl -- Designing PCR primers to amplify specific members or subgroups of multigene families / Robert M. Horton, Raghavanpillai Raju, Bianca M. Conti-Fine -- Screening gene family-enriched cDNA sublibraries with an unamplified cDNA probe : focusing on moderately to abundantly expressed clones / Meimei Hu, Bruce A. White.

Print version record.

Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs.

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