Protein sequencing protocols / edited by Bryan John Smith.

Contributor(s): Smith, Bryan John
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 64.Publisher: Totowa, N.J. : Humana Press, ©1997Description: 1 online resource (xiv, 375 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592595501; 1592595502; 1280836717; 9781280836718; 9786610836710; 661083671XSubject(s): Nucleotide sequence -- Laboratory manuals | Base Sequence | Sequence Analysis | Genetic Structures | Molecular Structure | Genetic Techniques | Molecular Sequence Data | Genetic Phenomena | Investigative Techniques | Biochemical Phenomena | Documentation | Chemical Phenomena | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Information Services | Phenomena and Processes | Information Science | Nucleotide sequence | Eiwitten | Aminozuren | Sequenties | Wetenschappelijke technieken | eiwitten | proteins | aminozuursequenties | amino acid sequences | Proteins and Enzymes | Eiwitten en enzymenGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Protein sequencing protocols.DDC classification: 574.87/328 LOC classification: QP625.N89 | P76 1997Other classification: 35.75 | 35.71 Online resources: Click here to access online
Contents:
Strategies for handling polypeptides on a microscale / Bryan John Smith, Paul Tempst -- SDS polyacrylamide gel electrophoresis for N-terminal protein sequencing / Bryan John Smith -- Two-dimensional polyacrylamide gel electrophoresis for the separation of proteins for chemical characterization / Michael J. Dunn -- Electroblotting of proteins from polyacrylamide gels for chemical characterization / Michael J. Dunn -- Enzymatic cleavage of polypeptides / Bryan John Smith, Colin Wheeler -- Chemical cleavage of polypeptides / Bryan John Smith -- Preparation of glycopeptides / Chris W. Sutton, Jacqui A. O'Neill -- Selective isolation of the carboxy terminal peptide from a protein / Tomoko Hayashi Akiyama, Tatsuru Sasagawa -- Selective isolation of the amino-terminal peptide from [alpha]-amino blocked protein / Tomoko Hayashi Akiyama, Tatsuru Sasagawa -- Analytical and micropreparative capillary electrophoresis of peptides / Alan J. Smith -- Reverse-phase HPLC purification of peptides from natural sources for structural analysis / Chris Shaw -- High-performance liquid chromatography on-line derivative spectroscopy for the characterization of peptides with aromatic amino acid residues / Christoph W. Turck -- Hydrolysis of samples for amino acid analysis / Ian Davidson -- Amino acid analysis : precolumn derivatization methods / G. Brent Irvine -- Postcolumn amino acid analysis / Alan J. Smith -- Fast-atom bombardment and electrospray mass spectrometry of peptides, proteins, and glycoproteins / Fiona M. Greer, Howard R. Morris -- Peptide mass fingerprinting using MALDI-TOF mass spectrometry / Darryl J. Pappin -- N-terminal protein ladder sequencing / Rong Wang, Brian T. Chait -- The Dansyl-Edman method for manual peptide sequencing / John M. Walker -- The Dansyl method for identifying N-terminal amino acids / John M. Walker -- Identification of PTH-amino acids by high-performance liquid chromatography / Gregory A. Grant, Mark W. Crankshaw -- Protein sequencer maintenance and troubleshooting / Bryan Dunbar -- Identifying the carboxy terminal amino acid of a protein / Tomoko Hayashi Akiyama, Tatsuru Sasagawa -- Enzymatic and chemical methods for manual C-terminal peptide sequencing / Franca Casagranda, John F. Wilshire -- Automated methods for C-terminal protein sequencing / Jerome M. Bailey, Chad G. Miller -- Chemical modification of proteins for sequence analysis / Alex F. Carne, Sally U -- On-membrane deblocking of proteins / Hisashi Hirano, Setsuko Komatsu, Susumu Tsunasawa -- Analysis of sites of protein phosphorylation / Alastair Aitken, Michèle Learmonth -- Identification of sites of glycosylation / Chris W. Sutton, Jacqui A. O'Neill -- Quantification and location of disulfide bonds in proteins / Alastair Aitken, Michèle Learmonth.
Action note: digitized 2010 committed to preserveSummary: Bryan Smith draws together in Protein Sequencing Protocols a timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on masters of the procedures. These well-tested methods convey valuable lessons and insights into using automated sequencers and/or mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acid by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. They also deal with such troublesome problems as identifying sites of modification and handling very small samples. Protein Sequencing Protocols offers self-contained, step-by-step recipes that ensure easily reproducible results for those already doing sequencing, and for those who are still learning the methods, as well as for those already in one area but wishing to learn associated techniques. Its multiplicity of proven protocols will guide the challenged experimenter to successful results.
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Includes bibliographical references and index.

Strategies for handling polypeptides on a microscale / Bryan John Smith, Paul Tempst -- SDS polyacrylamide gel electrophoresis for N-terminal protein sequencing / Bryan John Smith -- Two-dimensional polyacrylamide gel electrophoresis for the separation of proteins for chemical characterization / Michael J. Dunn -- Electroblotting of proteins from polyacrylamide gels for chemical characterization / Michael J. Dunn -- Enzymatic cleavage of polypeptides / Bryan John Smith, Colin Wheeler -- Chemical cleavage of polypeptides / Bryan John Smith -- Preparation of glycopeptides / Chris W. Sutton, Jacqui A. O'Neill -- Selective isolation of the carboxy terminal peptide from a protein / Tomoko Hayashi Akiyama, Tatsuru Sasagawa -- Selective isolation of the amino-terminal peptide from [alpha]-amino blocked protein / Tomoko Hayashi Akiyama, Tatsuru Sasagawa -- Analytical and micropreparative capillary electrophoresis of peptides / Alan J. Smith -- Reverse-phase HPLC purification of peptides from natural sources for structural analysis / Chris Shaw -- High-performance liquid chromatography on-line derivative spectroscopy for the characterization of peptides with aromatic amino acid residues / Christoph W. Turck -- Hydrolysis of samples for amino acid analysis / Ian Davidson -- Amino acid analysis : precolumn derivatization methods / G. Brent Irvine -- Postcolumn amino acid analysis / Alan J. Smith -- Fast-atom bombardment and electrospray mass spectrometry of peptides, proteins, and glycoproteins / Fiona M. Greer, Howard R. Morris -- Peptide mass fingerprinting using MALDI-TOF mass spectrometry / Darryl J. Pappin -- N-terminal protein ladder sequencing / Rong Wang, Brian T. Chait -- The Dansyl-Edman method for manual peptide sequencing / John M. Walker -- The Dansyl method for identifying N-terminal amino acids / John M. Walker -- Identification of PTH-amino acids by high-performance liquid chromatography / Gregory A. Grant, Mark W. Crankshaw -- Protein sequencer maintenance and troubleshooting / Bryan Dunbar -- Identifying the carboxy terminal amino acid of a protein / Tomoko Hayashi Akiyama, Tatsuru Sasagawa -- Enzymatic and chemical methods for manual C-terminal peptide sequencing / Franca Casagranda, John F. Wilshire -- Automated methods for C-terminal protein sequencing / Jerome M. Bailey, Chad G. Miller -- Chemical modification of proteins for sequence analysis / Alex F. Carne, Sally U -- On-membrane deblocking of proteins / Hisashi Hirano, Setsuko Komatsu, Susumu Tsunasawa -- Analysis of sites of protein phosphorylation / Alastair Aitken, Michèle Learmonth -- Identification of sites of glycosylation / Chris W. Sutton, Jacqui A. O'Neill -- Quantification and location of disulfide bonds in proteins / Alastair Aitken, Michèle Learmonth.

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Bryan Smith draws together in Protein Sequencing Protocols a timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on masters of the procedures. These well-tested methods convey valuable lessons and insights into using automated sequencers and/or mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acid by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. They also deal with such troublesome problems as identifying sites of modification and handling very small samples. Protein Sequencing Protocols offers self-contained, step-by-step recipes that ensure easily reproducible results for those already doing sequencing, and for those who are still learning the methods, as well as for those already in one area but wishing to learn associated techniques. Its multiplicity of proven protocols will guide the challenged experimenter to successful results.

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