Protocols in molecular neurobiology / edited by Alan Longstaff, Patricia Revest.

Contributor(s): Longstaff, Alan | Revest, Patricia
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 13.Publisher: Totowa, N.J. : Humana Press, ©1992Description: 1 online resource (xvii, 373 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781592595006; 1592595006; 0896031993; 9780896031999; 9781489940193; 1489940197Subject(s): Molecular neurobiology -- Methodology | Neurobiology -- methods | Neurobiologie moléculaire -- Méthodologie | Molecular neurobiology -- Methodology | Moleculaire biologie | Zenuwstelsel | Wetenschappelijke techniekenGenre/Form: Laboratory Manual. | Fulltext. | Internet Resource. | Laboratory Manuals. Additional physical formats: Print version:: Protocols in molecular neurobiology.DDC classification: 599/.0188 LOC classification: QP356.2 | .P76 1992Other classification: 44.90 Online resources: Click here to access online
Contents:
Separation of large DNA molecules by pulsed-field gel electrophoresis / Duncan J. Shaw -- Isolation of genomic DNA from invertebrates / Erno Vreugdenhil and Mark G. Darlison -- Analysis of mitochondrial DNA mutations / Masashi Tanaka and Takayuki Ozawa -- Design and use of oligonucleotides / Alan N. Bateson and Mark G. Darlison -- Use of degenerate oligonucleotides for polymerase chain-reaction-based isolation of related DNA sequences / Tania E. Webb and Alan N. Bateson -- Isolation of photoreceptor cell-specific MEKA cDNA by differential hybridization / Che-Hui Kuo -- Generation of isoform-specific antisera from cloned cDNAs: application to multiple forms of the Na/K-ATPase expressed in rat brain / Andrew W. Shyjan and Robert Levenson -- Determination of transcriptional intitiation sites and their usage in the nervous system / Gary Weisinger, Joseph D. DeCristofaro and Edmund F. La Gamma -- Mapping of trans-acting factor binding in the nervous system / Gary Weisinger and Edmund F. La Gamma -- In situ hybridization to brain tissue sections using labeled single-strand complementary RNA probes / Giulio M. Pasinetti -- In situ hybridization histochemistry using alkaline phosphatase-labeled oligodeoxynucleotide probe / Hiroshi Kiyama, Piers C. Emson and Masaya Tohyama -- Identification of neuropeptide gene regulatory elements in transgenic mice / Malcolm J. Low -- Expression of exogenous ion channels and neurotransmitter receptors in RNA-injected Xenopus oocytes / Nathan Dascal and Ilana Lotan -- Analysis of insulin and insulin-like growth factor-I receptors in neural tissues / Martin L. Adamo [and others] -- Use of affinity chromatography in purification of A₁ adenosine receptors from rat brain membranes / Hiroyasu Nakata -- Purification and structure of L-type calcium channels / Toni Schneider [and others] -- Purification and reconstitution of the ryanodine-sensitive Ca² release channel complex from muscle sarcoplasmic reticulum / F. Anthony Lai and Gerhard Meisner -- Identification of a lignad-gated ion channel by photoaffinity labeling and microsequencing / Ferdinand Hucho -- Voltage-gated ion channels: electrophysiological approaches / Nicholas B. Standen and Peter R. Stanfield -- Electrophysiological approach to the regulation of neuronal voltage-activated calcium channels by guanine nucleotide binding proteins / Roderick H. Scott and Annette C. Dolphin.
Action note: digitized 2010 committed to preserveSummary: Neurobiologists are bound to differ in their perceptions of what the discipline of molecular neurobiology should encompass. We have taken the view that molecular neurobiology should cover any aspect of brain science that uses the techniques of modern molecular biology, though we accept the fact that classification of a technique as a "biochemical* or "molecular biological* one is in itself somewhat arbitrary. Each chapter of this volume sets out to identify a clear problem in neurobiology and to place it in its context within the literature- i.e., indicating how the solution of the problem will advance knowledge in the field. The core of the chapter then details the approaches taken to solve the problem, in sufficient detail that the reader can appreciate exactly why a specific strategy was adopted and how it was executed. Each chapter also includes detailed protocols providing all the information necessary to reproduce the technique and its results in any appropriately equipped laboratory.
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Includes bibliographical references and index.

Separation of large DNA molecules by pulsed-field gel electrophoresis / Duncan J. Shaw -- Isolation of genomic DNA from invertebrates / Erno Vreugdenhil and Mark G. Darlison -- Analysis of mitochondrial DNA mutations / Masashi Tanaka and Takayuki Ozawa -- Design and use of oligonucleotides / Alan N. Bateson and Mark G. Darlison -- Use of degenerate oligonucleotides for polymerase chain-reaction-based isolation of related DNA sequences / Tania E. Webb and Alan N. Bateson -- Isolation of photoreceptor cell-specific MEKA cDNA by differential hybridization / Che-Hui Kuo -- Generation of isoform-specific antisera from cloned cDNAs: application to multiple forms of the Na/K-ATPase expressed in rat brain / Andrew W. Shyjan and Robert Levenson -- Determination of transcriptional intitiation sites and their usage in the nervous system / Gary Weisinger, Joseph D. DeCristofaro and Edmund F. La Gamma -- Mapping of trans-acting factor binding in the nervous system / Gary Weisinger and Edmund F. La Gamma -- In situ hybridization to brain tissue sections using labeled single-strand complementary RNA probes / Giulio M. Pasinetti -- In situ hybridization histochemistry using alkaline phosphatase-labeled oligodeoxynucleotide probe / Hiroshi Kiyama, Piers C. Emson and Masaya Tohyama -- Identification of neuropeptide gene regulatory elements in transgenic mice / Malcolm J. Low -- Expression of exogenous ion channels and neurotransmitter receptors in RNA-injected Xenopus oocytes / Nathan Dascal and Ilana Lotan -- Analysis of insulin and insulin-like growth factor-I receptors in neural tissues / Martin L. Adamo [and others] -- Use of affinity chromatography in purification of A₁ adenosine receptors from rat brain membranes / Hiroyasu Nakata -- Purification and structure of L-type calcium channels / Toni Schneider [and others] -- Purification and reconstitution of the ryanodine-sensitive Ca² release channel complex from muscle sarcoplasmic reticulum / F. Anthony Lai and Gerhard Meisner -- Identification of a lignad-gated ion channel by photoaffinity labeling and microsequencing / Ferdinand Hucho -- Voltage-gated ion channels: electrophysiological approaches / Nicholas B. Standen and Peter R. Stanfield -- Electrophysiological approach to the regulation of neuronal voltage-activated calcium channels by guanine nucleotide binding proteins / Roderick H. Scott and Annette C. Dolphin.

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English.

Neurobiologists are bound to differ in their perceptions of what the discipline of molecular neurobiology should encompass. We have taken the view that molecular neurobiology should cover any aspect of brain science that uses the techniques of modern molecular biology, though we accept the fact that classification of a technique as a "biochemical* or "molecular biological* one is in itself somewhat arbitrary. Each chapter of this volume sets out to identify a clear problem in neurobiology and to place it in its context within the literature- i.e., indicating how the solution of the problem will advance knowledge in the field. The core of the chapter then details the approaches taken to solve the problem, in sufficient detail that the reader can appreciate exactly why a specific strategy was adopted and how it was executed. Each chapter also includes detailed protocols providing all the information necessary to reproduce the technique and its results in any appropriately equipped laboratory.

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