Immobilization of enzymes and cells / edited by Gordon F. Bickerstaff.

Includes bibliographical references and index.

Print version record.

This collection of today's most significant immobilization methods reproduces those procedures currently and widely used in both industrial and research laboratories. These unfailingly reproducible techniques are so crafted that users can easily incorporate them into their existing methods and processes. Extensive notes provide additional information that helps extend the flexibility of the method.

English.

Immobilization of Enzymes and Cells: Some Practical Considerations / Gordon F. Bickerstaff -- Immobilization of Enzymes by Selective Adsorption on Biotinylaminopropyl Celite or Glass / Harold E. Swaisgood, Xiaolin L. Huang, Marie K. Walsh -- Immobilization of Proteins on Thionyl Chloride-Activated Controlled-Pore Glass / Violeta G. Janolino, Harold E. Swaisgood -- Enzyme Immobilization on Nylon / Pedro Lozano, José L. Iborra -- Visual Assessment of Enzyme Immobilization / Arturo Manjón, José L. Iborra -- Immobilization in Carrageenans / José L. Iborra, Arturo Manjón, Manuel Cánovas -- Entrapment in Calcium Alginate / Jane E. Fraser, Gordon F. Bickerstaff -- Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports / Maria Cantarella, Francesco Alfani, Laura Cantarella, Alberto Gallifuoco -- Activation of Rayon/Polyester Cloth for Protein Immobilization / Hiroshi Yamazaki, C. Scott Boyd -- Immobilization of Enzymes on Microelectrodes Using Chemical Crosslinking / Milena Koudelka-Hep, Nico F. Rooij, David J. Strike -- Photolithographic Patterning of Enzyme Membranes for the Modification of Microelectrodes / Milena Koudelka-Hep, Nico F. Rooij, David J. Strike -- Electrochemical-Based Immobilization of Enzymes / David J. Strike, Nico F. Rooij, Milena Koudelka-Hep -- Immobilization of Enzymes on Thermo-Responsive Polymers / Kazuhiro Hoshino, Setuko Akakabe, Shoichi Morohashi, Toshisuke Sasakura -- Immobilization of Photosynthetic Membranes in an Albumin-Glutaraldehyde Crosslinked Matrix / Robert Carpentier -- Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization / Guy Fortier, Nicole Demers, Jacques Jean-Fran çois, Jean-Charles Gayet, Edith M. D'Urso -- Poly(Carbamoyl Sulfonate) Hydrogels / Andreas Muscat, Klaus-Dieter Vorlop -- Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles / Gilbert Bardeletti -- Immobilization of Enzymes and Proteins on Red Blood Cells / Laura Chiarantini, Mauro Magnani -- Cellulose Paper Support for Immobilization / Marion Paterson, John F. Kennedy -- Immobilization of Cells Using Electrostatic Droplet Generation / Mattheus F. Goosen, Eltag S. Mahmud, Abdullah S. Al-Ghafri, Hamad A. Al-Hajri, Yousuf S. Al-Sinani, Branko Bugarski -- Hepatocyte Immobilization in Agarose and Functional Integrity Testing / Hassan Farghali, Sixtus Hynie -- Immobilized Hepatocytes in Xenobiotic Biotransformation Studies / Sixtus Hynie, Ludmila Kamenikova, Hassan Farghali -- Immobilization of Liposomes and Proteoliposomes in Gel Beads / Eggert Brekkan, Qing Yang, Gerhard Viel, Per Lundahl -- Cell Immobilization with Phosphorylated Polyvinyl Alcohol (PVA) Gel / Kuo-Cheng Chen, Jer-Yiing Houng -- Covalent Immobilization of Enzymes to Graphitic Particles / Marco F. Cardosi -- Enzyme Immobilization Using Chitosan-Xanthan Complexes / Severian Dumitriu, Pierre Vidal, Esteban Chornet -- Calcium Alginate Film Formed on a Stainless Steel Mesh / Harold E. Swaisgood, Flavia M. Passos -- Preparation of Immobilized Subunits of a Multisubunit Enzyme / Gordon F. Bickerstaff -- Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes / Gordon F. Bickerstaff -- Immobilization of Enzymes Acting on Macromolecular Substrates: Reduction of Steric Problems / José M. Guisán, Guadalupe Penzol, Pilar Armisen, Agatha Bastida, Rosa M. Blanco, Roberto Fernandez-Lafuente, Eduardo García-Junceda -- Immobilization of Enzymes on Glyoxyl Agarose: Strategies for Enzyme Stabilization by Multipoint Attachment / José M. Guisán, Agatha Bastida, Rosa M. Blanco, Roberto Fernández-Lafuente, Eduardo García-Junceda -- Stabilization of Immobilized Enzymes by Chemical Modification with Polyfunctional Macromolecules / José M. Guisán, Verónica Rodriguez, Cristina M. Rosell, Gloria Soler, Agatha Bastida, Rosa M. Blanco, Roberto Fernández-Lafuente, Eduardo García-Junceda -- Covalent Immobilization of Enzymes Using Commercially Available CDI-Activated Agarose / George J. Piazza, Marjorie B. Medina -- Immobilization of Cells in Polyelectrolyte Complexes / Johanna Mansfeld, Horst Dautzenberg -- Coimmobilization of Enzymes and Cells / Johanna Mansfeld, Horst Dautzenberg -- Adsorption of Lipase on Inorganic Supports / José V. Sinisterra -- Immobilization of Enzymes on Inorganic Supports by Covalent Methods / José V. Sinisterra -- Use of Divalent Metal Ions Chelated to Agarose Derivatives for Reversible Immobilization of Proteins / Robert R. Beitle, Mohammad M. Ataai -- Transition Metal Methods for Immobilization of Enzymes and Cells / John F. Kennedy, Joaquim M. Cabral, Maria R. Kosseva, Marion Paterson.