Electroporation protocols : preclinical and clinical gene medicine / Shulin Li, editor.

Contributor(s): Li, Shulin, 1961-
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 423.Publisher: Totowa, N.J. : Humana Press, ©2008Description: 1 online resource (xxi, 524 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781597451949; 1597451940Subject(s): Electroporation | Gene therapy | Mobile genetic elements | Transfection | Genetic Therapy | Electrochemotherapy | Immunotherapy | Methods | Transfection | Electroporation | Neoplasms | Laboratory Manuals | Cytological Techniques | Electrochemical Techniques | Investigative Techniques | Recombination, Genetic | Biological Therapy | Drug Therapy | Immunomodulation | Clinical Laboratory Techniques | Disease | Genetic Engineering | Publication Formats | Gene Transfer Techniques | Genetic Phenomena | Genetic Techniques | Therapeutics | Publication Characteristics | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Phenomena and Processes | Electroporation | Gene therapy | Mobile genetic elements | Transfection | electroporatie | electroporation | gentherapie | gene therapy | geneesmiddeltoedieningssystemen | drug delivery systems | Pharmacology | FarmacologieGenre/Form: Electronic books. Additional physical formats: Print version:: Electroporation protocols.DDC classification: 571.96481 LOC classification: QH585.5.E48Online resources: Click here to access online
Contents:
pt. I. Basic concepts of DNA transfer via electroporation. Application of electroporation gene therapy: past, current, and future / Lluis M. Mir -- Mechanism by which electroporation mediates DNA migration and entry into cells and targeted tissues / Marie-Pierre Rols -- Applicator and electrode design for in vivo DNA delivery by electroporation / Dietmar Rabussay -- Electrode assemblies used for electroporation of cultured cells / Leda Raptis and Kevin L. Firth -- Formulations for DNA delivery via electroporation in vivo / Khursheed Anwer -- Overview of drug delivery and alternative methods to electroporation / Sek-Wen Hui -- Nanoelectroporation: a first look / Raji Sundararajan -- pt. II. In vitro targeted gene delivery via electroporation. Nucleofection of human embryonic stem cells / Henrike Siemen [and others] -- Delivery of whole tumor lysate into dendritic cells for cancer vaccination / Linda N. Liu [and others] -- Delivery of tumor-antigen-encoding mRNA into dendritic cells for vaccination / Annelies Michiels [and others].
Delivery of DNA into natural killer cells for immunotherapy / Kathrin Schoenberg, Hans-Ingo Trompeter, and Markus Uhrberg -- Electroporation of adherent cells in situ for the study of signal transduction and gap junctional communication / Leda Raptis [and others] -- Delivery of DNA into adipocytes within adipose tissue / James G. Granneman -- pt. III. In vivo targeted gene delivery via electroporation. Delivery of DNA into muscle for treating systemic diseases: advantages and challenges / Capucine Trollet, Daniel Scherman, and Pascal Bigey -- Delivery of DNA into skeletal muscle in large animals / Patricia A. Brown, Amir S. Khan, and Ruxandra Draghia-Akli -- Delivery of DNA into skin via electroporation / Babu M. Medi and Jagdish Singh -- Electroporation-mediated gene delivery to the lungs / Rui Zhou, James E. Norton, and David A. Dean -- Delivery of DNA into bladder via electroporation / Masaki Yoshida [and others] -- Analysis of gene function in the retina / Takahiko Matsuda and Constance L. Cepko -- Optical in vivo imaging of electrically mediated delivery of siRNA into muscle for gene function analysis / Muriel Golzio and Justin Teissie -- Electroporation of adult zebrafish / N. Madhusudhana Rao, K. Murali Rambabu, and S. Harinarayana Rao.
pt. IV. Treatment of cancer via electroporation gene therapy. Flow electroporation with pulsed electric fields for purging tumor cells / Abie Craiu and David Scadden -- Delivery of DNA into tumors / Shulin Li -- Intraturmoral bieomycin and IL-12 electrochemogenetherapy for treating head and neck tumors in dogs / Jeffry Cutrera [and others] -- Systemic IL-12 gene therapy for treating malignancy via intramuscular electroporation / Shiguo Zhu and Shulin Li -- Treatment of SCCVII tumors with systemic chemotherapy and interleukin-12 gene therapy combination / Marina Torrero and Shulin Li -- Electroporation for drug and gene delivery in the cliic: doctors go electric / Julie Gehl -- IL-2 plasmid electroporation: from preclinical studies in phase I clinical trial / Holly M. Horton, Peggy A. Lalor, and Alain P. Rolland -- pt. V. Treatment of other diseases via electroporation gene therapy. Factor IX gene therapy for hemophilia / James G. Fewell -- KGF-1 for wound healing in animal models / Guy P. Marti [and others] -- Hapatocyte growth factor gene therapy for hypertension / Kazuo Komamura [and others] -- Electroporation of corrective nucleic acids (CNA) in vivo to promote gene correction in dstrophic muscle / Robert M.I. Kapsa, Sharon H.A. Wong, and Anita F. Quigley.
Gene delivery to dystrophic muscle / Kim E. Wells [and others] -- Effect of electroporation on cardiac electrophysiology / Vadim V. Fedorov, Vladimir P. Nikolski, and Igor R. Efimov -- Muscle and fat mass modulation in different clinical models / Ruxandra Draghia-Akli and Amir S. Khan -- pt. VI. Applications of electroporation for DNA vaccination. DNA vaccination for prostate cancer / Anna-Karin Roos, Alan King, and Pavel Pisa -- HER2/neu DNA vaccination for breast tumors / Arianna Smorlesi [and others] -- Electroporation-mediated HBV DNA vaccination in primate models / Yong-Gang Zhao and Yuhong Xu -- Taking electroporation-based delivery of DNA vaccination into humans: a generic clinical protocol / Torunn Elisabeth Tjelle [and others] -- Production of monoclonal antibody by DNA immunization with electroporation / Kaw Yan Chua, John D.A. Ramos, and Nge Cheong.
Summary: At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinical and Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology. Ideal for beginners and essential for the more knowledgable, Electroporation Protocols: Preclinical and Clinical Gene Medicine collects a comprehensive set of the newest discoveries and applications in a revolutionary field that offers so much to our fight against disease.
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Includes bibliographical references and index.

pt. I. Basic concepts of DNA transfer via electroporation. Application of electroporation gene therapy: past, current, and future / Lluis M. Mir -- Mechanism by which electroporation mediates DNA migration and entry into cells and targeted tissues / Marie-Pierre Rols -- Applicator and electrode design for in vivo DNA delivery by electroporation / Dietmar Rabussay -- Electrode assemblies used for electroporation of cultured cells / Leda Raptis and Kevin L. Firth -- Formulations for DNA delivery via electroporation in vivo / Khursheed Anwer -- Overview of drug delivery and alternative methods to electroporation / Sek-Wen Hui -- Nanoelectroporation: a first look / Raji Sundararajan -- pt. II. In vitro targeted gene delivery via electroporation. Nucleofection of human embryonic stem cells / Henrike Siemen [and others] -- Delivery of whole tumor lysate into dendritic cells for cancer vaccination / Linda N. Liu [and others] -- Delivery of tumor-antigen-encoding mRNA into dendritic cells for vaccination / Annelies Michiels [and others].

Delivery of DNA into natural killer cells for immunotherapy / Kathrin Schoenberg, Hans-Ingo Trompeter, and Markus Uhrberg -- Electroporation of adherent cells in situ for the study of signal transduction and gap junctional communication / Leda Raptis [and others] -- Delivery of DNA into adipocytes within adipose tissue / James G. Granneman -- pt. III. In vivo targeted gene delivery via electroporation. Delivery of DNA into muscle for treating systemic diseases: advantages and challenges / Capucine Trollet, Daniel Scherman, and Pascal Bigey -- Delivery of DNA into skeletal muscle in large animals / Patricia A. Brown, Amir S. Khan, and Ruxandra Draghia-Akli -- Delivery of DNA into skin via electroporation / Babu M. Medi and Jagdish Singh -- Electroporation-mediated gene delivery to the lungs / Rui Zhou, James E. Norton, and David A. Dean -- Delivery of DNA into bladder via electroporation / Masaki Yoshida [and others] -- Analysis of gene function in the retina / Takahiko Matsuda and Constance L. Cepko -- Optical in vivo imaging of electrically mediated delivery of siRNA into muscle for gene function analysis / Muriel Golzio and Justin Teissie -- Electroporation of adult zebrafish / N. Madhusudhana Rao, K. Murali Rambabu, and S. Harinarayana Rao.

pt. IV. Treatment of cancer via electroporation gene therapy. Flow electroporation with pulsed electric fields for purging tumor cells / Abie Craiu and David Scadden -- Delivery of DNA into tumors / Shulin Li -- Intraturmoral bieomycin and IL-12 electrochemogenetherapy for treating head and neck tumors in dogs / Jeffry Cutrera [and others] -- Systemic IL-12 gene therapy for treating malignancy via intramuscular electroporation / Shiguo Zhu and Shulin Li -- Treatment of SCCVII tumors with systemic chemotherapy and interleukin-12 gene therapy combination / Marina Torrero and Shulin Li -- Electroporation for drug and gene delivery in the cliic: doctors go electric / Julie Gehl -- IL-2 plasmid electroporation: from preclinical studies in phase I clinical trial / Holly M. Horton, Peggy A. Lalor, and Alain P. Rolland -- pt. V. Treatment of other diseases via electroporation gene therapy. Factor IX gene therapy for hemophilia / James G. Fewell -- KGF-1 for wound healing in animal models / Guy P. Marti [and others] -- Hapatocyte growth factor gene therapy for hypertension / Kazuo Komamura [and others] -- Electroporation of corrective nucleic acids (CNA) in vivo to promote gene correction in dstrophic muscle / Robert M.I. Kapsa, Sharon H.A. Wong, and Anita F. Quigley.

Gene delivery to dystrophic muscle / Kim E. Wells [and others] -- Effect of electroporation on cardiac electrophysiology / Vadim V. Fedorov, Vladimir P. Nikolski, and Igor R. Efimov -- Muscle and fat mass modulation in different clinical models / Ruxandra Draghia-Akli and Amir S. Khan -- pt. VI. Applications of electroporation for DNA vaccination. DNA vaccination for prostate cancer / Anna-Karin Roos, Alan King, and Pavel Pisa -- HER2/neu DNA vaccination for breast tumors / Arianna Smorlesi [and others] -- Electroporation-mediated HBV DNA vaccination in primate models / Yong-Gang Zhao and Yuhong Xu -- Taking electroporation-based delivery of DNA vaccination into humans: a generic clinical protocol / Torunn Elisabeth Tjelle [and others] -- Production of monoclonal antibody by DNA immunization with electroporation / Kaw Yan Chua, John D.A. Ramos, and Nge Cheong.

Print version record.

At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinical and Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology. Ideal for beginners and essential for the more knowledgable, Electroporation Protocols: Preclinical and Clinical Gene Medicine collects a comprehensive set of the newest discoveries and applications in a revolutionary field that offers so much to our fight against disease.

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