Neural stem cells : methods and protocols / edited by Leslie P. Weiner.
Contributor(s): Weiner, Leslie PMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 438.Publisher: Totowa, N.J. : Humana Press, ©2008Edition: 2nd edDescription: 1 online resource (xv, 406 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781597451338; 1597451339Subject(s): Neurons -- Laboratory manuals | Stem cells -- Laboratory manuals | Neurons -- cytology | Stem Cells -- cytology | Cytological Techniques | Stem Cells -- physiology | Neurons | Stem cells | neurologie | neurology | moleculaire biologie | molecular biology | celdifferentiatie | cell differentiation | neurobiologie | neurobiology | stamcellen | stem cells | Neurophysiology | Molecular Biology (General) | Neurofysiologie | Moleculaire biologie (algemeen)Genre/Form: Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Neural stem cells.DDC classification: 611/.0188 LOC classification: QP357 | .N48 2008Online resources: Click here to access online
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Includes bibliographical references and index.
Definitions and criteria for stem cells / Leslie P. Weiner -- Neural stem cells and the future treatment of neurological diseases : raising the standard / Jaime Imitola and Samia J. Khoury -- Neural differentiation of human embryonic stem cells / Mirella Dottori and Martin F. Pera -- Generating neurons from stem cells / Andreas Androutsellis-Theotokis [and others] -- Isolation of neural stem and precursor cells from rodent tissue / Yuanyuan Wu [and others] -- Adult rodent spinal cord-derived neural stem cells : isolation and characterization / Lamya S. Shihabuddin -- Adult neural stem cells / Rossella Galli, Angela Gritti, and Angelo L. Vescovi -- Olfactory ensheathing cells : isolation and culture from the neonatal olfactory bulb / Susan C. Barnett and A. Jane Roskams -- Culturing olfactory ensheathing cells from the mouse olfactory epithelium / Miranda Richter, Kathryn Westendorf, and A. Jane Roskams -- Production and characterization of immortal human neural stem cell line with multipotent differentiation property / Seung U. Kim [and others] -- Preparation of neural progenitors from bone marrow and umbilical cord blood / Shijie Song and Juan Sanchez-Ramos.
Production of neurospheres from CNS tissue / Gregory P. Marshall II [and others] -- Nuclear transfer to study the nuclear reprogramming of human stem cells / Shigeo Saito [and others] -- Clonal analyses and cryopreservation of neural stem cell cultures / Angela Gritti, Rossella Galli, and Angelo L. Vescovi -- Telomere neurobiology / Mark P. Mattson, Peisu Zhang, and Aiwu Cheng -- Chromosomal mosaicism in neural stem cells / Suzanne E. Peterson [and others] -- In vitro assays for neural stem cell differentiation : induction of dopaminergic phenotype / Marcel M. Daadi -- In vitro migration assays of neural stem cells / Pascale Durbec . [and others] -- Measuring apoptosis in neural stem cells / Brett T. Lund and Eve E. Kelland -- Markers of adult neural stem cells / Eric Wexler -- Differential gene expression in ES-derived neural stem cells by using RT-PCR / Nicole Slawny, Crystal Pacut, and Theresa E. Gratsch -- Microfluidic devices for high-throughput gene expression profiling of single hESC-derived neural stem cells / Yan Chen and Jiang F. Zhong.
Electroporation strategies for genetic manipulation and cell labeling / Terence J. Van Raay, Rhonda T. Lassiter, and Michael R. Stark -- Novel and immortalization-based protocols for the generation of neural CNS stem cell lines for gene therapy approaches / Luciano Conti and Elena Cattaneo -- Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo / Sanjay S. Magavi and Jeffrey D. Macklis -- Immunocytochemical analysis of neuronal differentiation / Sanjay S. Magavi and Jeffrey D. Macklis -- Neuroanatomical tracing of neuronal projections with Fluoro-Gold / Lisa A. Catapano, Sanjay S. Magavi, and Jeffrey D. Macklis -- Labeling stem cells in vitro for identification of their differentiated phenotypes after grafting into the CNS / Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore -- Optimizing stem cell grafting into the CNS / Scott R. Whittemore [and others] -- Routes of stem cell administration in the adult rodent / Alison E. Willing [and others].
Print version record.
Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.