Osteoporosis : methods and protocols / Jennifer J. Westendorf, editor.

Contributor(s): Westendorf, Jennifer J
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 455.Publisher: Totowa, N.J. : Humana Press, ©2008Description: 1 online resource (xix, 354 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781597451048; 1597451045Subject(s): Osteoporosis -- Molecular aspects -- Laboratory manuals | Osteoporosis -- Genetic aspects -- Laboratory manuals | Bones -- Cytology -- Laboratory manuals | Bone and Bones -- pathology | Osteoporosis -- genetics | Musculoskeletal System Diseases | Biophysics | Medicine | Biology | Health & Biological Sciences | botontkalking | osteoporosis | botziekten | bone diseases | moleculaire biologie | molecular biology | laboratoriummethoden | laboratory methods | biologische technieken | biological techniques | protocollen | protocols | genexpressieanalyse | genomics | Medicine (General) | Biological Techniques | Geneeskunde (algemeen) | Biologische techniekenGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: Osteoporosis.DDC classification: 616.7/1 LOC classification: RC931.O73 | O88 2008Online resources: Click here to access online
Contents:
In vitro models. Isolation and culture of rodent osteoprogenitor cells / Thomas A. Owen and Lydia C. Pan. Osteoclast culture and resorption assays / Elizabeth W. Bradley and Merry Jo Oursler. Assessing new bone formation in neonatal calvarial organ cultures / Khalid S. Mohammad, John M. Chirgwin, and Theresa A. Guise. Detection of apoptosis of bone cells in vitro / Teresita Bellido and Lilian I. Plotkin -- In vivo rodent models. In vivo parathyroid hormone treatments and RNA isolation and analysis / Xin Li, Ling Qin, and Nicola C. Partridge. Assessment of bone formation capacity using in vivo transplantation assays : procedure and tissue analysis / Basem M. Abdallah, Nicholas Ditzel, and Moustapha Kassem. Ossicle and vossicle implant model systems / Glenda J. Pettway and Laurie K. McCauley. Utility of the ovariectomized rat as a model for human osteoporosis in drug discovery / Yogendra P. Kharode, Michael C. Sharp, and Peter V.N. Bodine. Orchidectomy models of osteoporosis / Stéphane Blouin [and others] -- Methods of gene delivery. Gene delivery by adenoviruses / Renny T. Franceschi and Chunxi Ge. Lentivirus delivery of shRNA constructs into osteoblasts / Marc N. Wein, Dallas C. Jones, and Laurie H. Glimcher. Gene delivery by retroviruses / Valerie Deregowski and Ernesto Canalis -- Molecular and genetic techniques. Chromatin immunoprecipitation assays : application of ChIP-on-chip for defining dynamic transcriptional mechanisms in bone cells / Margaretha van der Deen [and others] Identification of transcription factor target genes by ChIP display / Artem Barski, Steven Pregizer, and Baruch Frenkel. Application of the laser capture microdissection technique for molecular definition of skeletal cell differentiation in vivo / Dafna Benayahu, Rina Socher, and Irena Shur. Quantitative trait loci mapping / Dong-Hai Xiong [and others] -- Imaging techniques. In situ nuclear organization of regulatory machinery / Shirwin M. Pockwinse [and others] Bioluminescent imaging in bone / Yoram Zilberman [and others] Micro-computed tomography : a method for the non-destructive evaluation of the three-dimensional structure of biological specimens / Martin Stauber and Ralph Müller. Fourier transform-infrared microspectrosopy and microscopic imaging / Samuel Gourion-Arsiquaud, Paul A. West, and Adele L. Boskey. Mechanical testing. Assessment of the in vivo adaptive response to mechanical loading / Leanne Kaye Saxon and Lance Edward Lanyon. Determination of bone's mechanical matrix properties by nanoindentation / Engin Ozcivici [and others] Fluid flow assays / Ryan C. Riddle, Amanda F. Taylor and Henry J. Donahue.
Summary: In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more. As a volume in the highly successful Methods in Molecular Biology series, each chapter provides a brief introduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Osteoporosis: Methods and Protocols is the perfect guide for both new and experienced experimentalists attempting to study the devastating silent disease known as osteoporosis.
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Includes bibliographical references and index.

In vitro models. Isolation and culture of rodent osteoprogenitor cells / Thomas A. Owen and Lydia C. Pan. Osteoclast culture and resorption assays / Elizabeth W. Bradley and Merry Jo Oursler. Assessing new bone formation in neonatal calvarial organ cultures / Khalid S. Mohammad, John M. Chirgwin, and Theresa A. Guise. Detection of apoptosis of bone cells in vitro / Teresita Bellido and Lilian I. Plotkin -- In vivo rodent models. In vivo parathyroid hormone treatments and RNA isolation and analysis / Xin Li, Ling Qin, and Nicola C. Partridge. Assessment of bone formation capacity using in vivo transplantation assays : procedure and tissue analysis / Basem M. Abdallah, Nicholas Ditzel, and Moustapha Kassem. Ossicle and vossicle implant model systems / Glenda J. Pettway and Laurie K. McCauley. Utility of the ovariectomized rat as a model for human osteoporosis in drug discovery / Yogendra P. Kharode, Michael C. Sharp, and Peter V.N. Bodine. Orchidectomy models of osteoporosis / Stéphane Blouin [and others] -- Methods of gene delivery. Gene delivery by adenoviruses / Renny T. Franceschi and Chunxi Ge. Lentivirus delivery of shRNA constructs into osteoblasts / Marc N. Wein, Dallas C. Jones, and Laurie H. Glimcher. Gene delivery by retroviruses / Valerie Deregowski and Ernesto Canalis -- Molecular and genetic techniques. Chromatin immunoprecipitation assays : application of ChIP-on-chip for defining dynamic transcriptional mechanisms in bone cells / Margaretha van der Deen [and others] Identification of transcription factor target genes by ChIP display / Artem Barski, Steven Pregizer, and Baruch Frenkel. Application of the laser capture microdissection technique for molecular definition of skeletal cell differentiation in vivo / Dafna Benayahu, Rina Socher, and Irena Shur. Quantitative trait loci mapping / Dong-Hai Xiong [and others] -- Imaging techniques. In situ nuclear organization of regulatory machinery / Shirwin M. Pockwinse [and others] Bioluminescent imaging in bone / Yoram Zilberman [and others] Micro-computed tomography : a method for the non-destructive evaluation of the three-dimensional structure of biological specimens / Martin Stauber and Ralph Müller. Fourier transform-infrared microspectrosopy and microscopic imaging / Samuel Gourion-Arsiquaud, Paul A. West, and Adele L. Boskey. Mechanical testing. Assessment of the in vivo adaptive response to mechanical loading / Leanne Kaye Saxon and Lance Edward Lanyon. Determination of bone's mechanical matrix properties by nanoindentation / Engin Ozcivici [and others] Fluid flow assays / Ryan C. Riddle, Amanda F. Taylor and Henry J. Donahue.

Print version record.

In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more. As a volume in the highly successful Methods in Molecular Biology series, each chapter provides a brief introduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Osteoporosis: Methods and Protocols is the perfect guide for both new and experienced experimentalists attempting to study the devastating silent disease known as osteoporosis.

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