HIV protocols / edited by Vinayaka R. Prasad and Ganjam V. Kalpana.

Contributor(s): Prasad, Vinayaka R | Kalpana, Ganjam V
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 485.; Springer protocols: Publisher: New York, NY : Humana Press, ©2009Edition: 2nd edDescription: 1 online resource (xiv, 457 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781597451703; 1597451703Subject(s): AIDS (Disease) -- Laboratory manuals | HIV (Viruses) -- Laboratory manuals | HIV infections -- Laboratory manuals | Clinical Protocols | HIV Infections -- prevention & control | Immunologic Techniques | Molecular Biology -- methods | Virology -- methods | AIDS (Disease) | HIV infections | HIV (Viruses) | Microbiology & Immunology | Biophysics | Biology | Health & Biological Sciences | human immunodeficiency viruses | acquired immune deficiency syndrome | retrovirale vectoren | retroviral vectors | laboratoriummethoden | laboratory methods | protocollen | protocols | Virology | Immunology | Human Pathology | Virologie | Immunologie | Humane pathologieGenre/Form: Laboratory Manual. | Electronic books. | Laboratory manuals. Additional physical formats: Print version:: HIV protocols.DDC classification: 616.079 LOC classification: QR201.A37Online resources: Click here to access online
Contents:
Methods for viral RNA isolation and PCR amplification for sequencing of near full-length HIV-1 genomes / K.S. Kemal [and others]-Purification of HIV-1 virions by subtilisin digestion or CD45 immunoaffinity depletion for biochemical studies / D.E. Ott-Calculating HIV-1 infectious titre using a virtual TCID50 method / Y. Gao [and others]-Cell-free assays for HIV-1 uncoating / C. Aiken-Real-time pcr analysis of HIV-1 replication post-entry events / J.L. Mbisa [and others]-Analysis of 2-LTR circle junctions of viral DNA in infected cells / D. Mandal and V.R. Prasad-HIV-1 recombination: An experimental assay and a phylogenetic approach / M.D. Moore, M.P. Chin and W.S. Hu-Methods of preparation and analysis of intracellular reverse transcription complexes / A. Fassati-Analysis of viral and cellular proteins in HIV-1 reverse transcription complexes by co-immunoprecipitation / S.N. Iordanskiy and M.I. Bukrinsky-Isolation and analysis of HIV-1 preintegration complexes / A. Engelman-Bisarsenical labeling of HIV-1 for real-time fluorescence microscopy / N.J. Arhel and P. Charneau-Methods for the study of HIV-1 assembly / A.A. Waheed, A. Ono and E.O. Freed-Assembly of immature HIV-1 capsids using a cell-free system / J.R. Lingappa and B.K. Thielen-Preparation of recombinant HIV-1 gag protein and assembly of virus-like particles in vitro / S.A. Datta and A. Rein-Methods for the analysis of HIV-1 nucleocapsid protein interactions with oligonucleotides / A.G. Stephen and R.J. Fisher-Methods for analysis of incorporation and annealing methods for analysis of tRNA(lys) in HIV-1 / S. Cen, F. Guo and L. Kleiman-Somatic cell genetic analyses to identify HIV-1 host restriction factors / S.T. Valente and S.P. Goff-Rapid, controlled and intensive lentiviral vector-based RNAi / M. Llano, N. Gaznick and E.M. Poeschla-Reverse two-hybrid screening to analyze protein-protein interaction of HIV-1 viral and cellular proteins / S. Das and G.V. Kalpana-Methods to study monocyte migration induced by HIV-infected cells / V.R. Rao [and others]-Novel mouse models for understanding HIV-1 pathogenesis / A. Joseph, K. Sango and H. Goldstein-Mucosal antibody responses to HIV / Z. Moldoveanu and J. Mestecky-Isolating mucosal lymphocytes from biopsy tissue for cellular immunology assays / B.L. Shacklett, J.W. Critchfield and D. Lemongello-Quantifying HIV-1-specific CD8 (+) T-Cell responses using elispot and cytokine flow cytometry / B.L. Shacklett, J.W. Critchfield and D. Lemongello-Multiparameter flow cytometry monitoring of t cell responses / H.T. Maecker-Measuring HIV neutralization in a luciferase reporter gene assay / D.C. Montefiori-Assessing the antiviral activity of HIV-1-specific cytotoxic t lymphocytes / O.O. Yang-Methods for quantitating antigen-specific t cell responses using functional assays in rhesus macaques / R.R. Amara-Isolation of drug-resistant mutant HIV variants using tissue culture drug selection / M. Oliveira, B.G. Brenner and M.A. Wainberg-Virus evolution as a tool to study HIV-1 biology / B. Berkhout and A.T. Das.
Summary: Despite major advances in the treatment of HIV, there are still many areas requiring additional study. These areas include ways to create efficacious, potent antiretrovirals for suppressing viral loads completely without toxic side effects, the definition of unknown drug targets while fine-tuning known targets, and the ability to better understand the interplay between viral and host factors. In HIV Protocols, Second Edition, expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, HIV Protocols, Second Edition is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1.
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Includes bibliographical references and index.

Methods for viral RNA isolation and PCR amplification for sequencing of near full-length HIV-1 genomes / K.S. Kemal [and others]-Purification of HIV-1 virions by subtilisin digestion or CD45 immunoaffinity depletion for biochemical studies / D.E. Ott-Calculating HIV-1 infectious titre using a virtual TCID50 method / Y. Gao [and others]-Cell-free assays for HIV-1 uncoating / C. Aiken-Real-time pcr analysis of HIV-1 replication post-entry events / J.L. Mbisa [and others]-Analysis of 2-LTR circle junctions of viral DNA in infected cells / D. Mandal and V.R. Prasad-HIV-1 recombination: An experimental assay and a phylogenetic approach / M.D. Moore, M.P. Chin and W.S. Hu-Methods of preparation and analysis of intracellular reverse transcription complexes / A. Fassati-Analysis of viral and cellular proteins in HIV-1 reverse transcription complexes by co-immunoprecipitation / S.N. Iordanskiy and M.I. Bukrinsky-Isolation and analysis of HIV-1 preintegration complexes / A. Engelman-Bisarsenical labeling of HIV-1 for real-time fluorescence microscopy / N.J. Arhel and P. Charneau-Methods for the study of HIV-1 assembly / A.A. Waheed, A. Ono and E.O. Freed-Assembly of immature HIV-1 capsids using a cell-free system / J.R. Lingappa and B.K. Thielen-Preparation of recombinant HIV-1 gag protein and assembly of virus-like particles in vitro / S.A. Datta and A. Rein-Methods for the analysis of HIV-1 nucleocapsid protein interactions with oligonucleotides / A.G. Stephen and R.J. Fisher-Methods for analysis of incorporation and annealing methods for analysis of tRNA(lys) in HIV-1 / S. Cen, F. Guo and L. Kleiman-Somatic cell genetic analyses to identify HIV-1 host restriction factors / S.T. Valente and S.P. Goff-Rapid, controlled and intensive lentiviral vector-based RNAi / M. Llano, N. Gaznick and E.M. Poeschla-Reverse two-hybrid screening to analyze protein-protein interaction of HIV-1 viral and cellular proteins / S. Das and G.V. Kalpana-Methods to study monocyte migration induced by HIV-infected cells / V.R. Rao [and others]-Novel mouse models for understanding HIV-1 pathogenesis / A. Joseph, K. Sango and H. Goldstein-Mucosal antibody responses to HIV / Z. Moldoveanu and J. Mestecky-Isolating mucosal lymphocytes from biopsy tissue for cellular immunology assays / B.L. Shacklett, J.W. Critchfield and D. Lemongello-Quantifying HIV-1-specific CD8 (+) T-Cell responses using elispot and cytokine flow cytometry / B.L. Shacklett, J.W. Critchfield and D. Lemongello-Multiparameter flow cytometry monitoring of t cell responses / H.T. Maecker-Measuring HIV neutralization in a luciferase reporter gene assay / D.C. Montefiori-Assessing the antiviral activity of HIV-1-specific cytotoxic t lymphocytes / O.O. Yang-Methods for quantitating antigen-specific t cell responses using functional assays in rhesus macaques / R.R. Amara-Isolation of drug-resistant mutant HIV variants using tissue culture drug selection / M. Oliveira, B.G. Brenner and M.A. Wainberg-Virus evolution as a tool to study HIV-1 biology / B. Berkhout and A.T. Das.

Print version record.

Despite major advances in the treatment of HIV, there are still many areas requiring additional study. These areas include ways to create efficacious, potent antiretrovirals for suppressing viral loads completely without toxic side effects, the definition of unknown drug targets while fine-tuning known targets, and the ability to better understand the interplay between viral and host factors. In HIV Protocols, Second Edition, expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, HIV Protocols, Second Edition is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1.

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