Molecular biomethods handbook / edited by John M. Walker, Ralph Rapley.

Contributor(s): Walker, John M, 1948- | Rapley, Ralph
Material type: TextTextPublisher: Totowa, NJ : Humana Press, ©2008Edition: 2nd edDescription: 1 online resource (xx, 1124 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781603273756; 1603273751Subject(s): Molecular biology -- Methodology | Molecular diagnosis -- Methodology | Chemistry Techniques, Analytical | Biochemistry | Clinical Laboratory Techniques | Genetics | Investigative Techniques | Analytical, Diagnostic and Therapeutic Techniques and Equipment | Chemistry | Biological Science Disciplines | Biology | Natural Science Disciplines | Disciplines and Occupations | Molecular Biology | Genetic Techniques | Chemistry Techniques, Analytical -- methods | Cytological Techniques | Biologie moléculaire | Biologie moléculaire -- Technique | SCIENCE -- Life Sciences -- Molecular Biology | Biomédecine | Sciences de la vie | Molecular biology -- Methodology | Biochemie | Labortechnik | Methode | Molekularbiologie | moleculaire biologie | molecular biology | biologische technieken | biological techniques | methodologie | methodology | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Electronic books. | Handbooks and manuals. | Laboratory manuals. Additional physical formats: Print version:: Molecular biomethods handbook.DDC classification: 572.8 LOC classification: QH506 | .M6642 2008Online resources: Click here to access online
Contents:
Nucleic acid methods: The manipulation of nucleic acids : basic tools and techniques / Gayle Corkill and Ralph Rapley. Restriction enzymes : tools in clinical research / Gareth J.S. Jenkins. Principles and medical applications of the polymerase chain reaction / Bimal D.M. Theophilus. Probe design, production, and applications / Marilena Aquino de Muro. Southern blotting as a diagnostic method / Bronwen M. Harvey and Pirkko Soundy. Capillary electrophoresis of DNA : biomedical applications / Beatriz Sanchez-Vega. Denaturing high-performance liquid chromatography (DHPLC) for nucleic acid analysis / Kim Hung Leung and Shea Ping Yip. Denaturing gradient gel electrophoresis (DGGE) / Jeroen H. Roelfsema and Dorien J.M. Peters. Single strand conformation polymorphism (SSCP) analysis / Kim Hung Leung and Shea Ping Yip. Randomly amplified polymorphic DNA (RAPD) : a useful tool for genomic characterization of different organisms / Lúcia Maria da Cunha Galvão and Eliane Lages-Silva. Quantification of mRNA using real time RT-PCR : the SYBR solution / David Sugden and Patricia de Winter. Quantitative analysis of DNA sequences by PCR and solid-phase minisequencing / Anu Suomalainen and Ann-Christine Syvänen. Multiplex amplifiable probe hybridization (MAPH) / Carolina Sismani, Ludmila Kousoulidou, and Philippos C. Patsalis. Gene expression profiling / Arnis Druka, Robbie Waugh, and Pete Hedley. Comparative genomic hybridization in clinical and medical research / Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin. Subtractive hybridization / Craig Winstanley. Fluorescence in situ hybridization / Jane Bayani and Jeremy A. Squire. Quantitative trait locus mapping to identify genes for complex traits in mice / Jonathan D. Smith. cDNA microarrays / Phillip G. Febbo. Mapping techniques / Simon G. Gregory. Single nucleotide polymorphisms / Nameeta Shah. Gene/protein sequence analysis : a compilation of bioinformatic tools / Bernd H.A. Rehm and Frank Reinecke.
Protein and cell methods: Protein electrophoresis / David Sheehan and Siobhan O'Sullivan. Protein blotting / Patricia Gravel. Capillary electrophoresis of proteins / Mark Strege. Autoradiograph and fluorography / Bronwen M. Harvey. Mass spectrometry of proteins and peptides / Kenneth G. Standing. Post-translational modifications of proteins / Christoph Kannich and Birte Fuchs. Protein microarray technology / Charlotte H. Clarke and Eric T. Fung. Protein-protein interactions / Hae Ryoun Park [and others]. Glycoprotein analysis / Terry D. Butters and David C.A. Neville. Solid-phase peptide synthesis / Mare Cudic and Gregg B. Fields. Monoclonal antibodies / Zhong J. Zhang and Maher Albitar. Antibody phage display / Rob Aitken. Protein engineering / Thomas Willemsen [and others]. Directed protein evolution / Sabine C. Stebel [and others]. Enzyme linked immunosorbent assay (ELISA) / John Crowther. Epitope mapping : identification of antibody-binding sites on protein antigens / Glenn E. Morris. Quantum dots / Charles Z. Hotz. Ion-exchange chromatography / David Sheehan. Size-exclusion chromatography / Paul Cutler. Hydrophobic interaction chromatography / Paul A. O'Farrell. Affinity chromatography / Adam Charlton and Michael Zachariou. High performance liquid chromatography (HPLC) of peptides and proteins / Tzong-Hsien Lee and Marie-Isabel Aguilar. Amino acid analysis / Ian Davidson and Paula O'Connor. Surface plasmon resonance / K. Scott Phillips and Quan Jason Cheng. Macromolecular crystallography / Bernhard Rupp and Katherine A. Kantardjieff. Microchip devices for bioanalysis / Anna C. Kinsella and Shelley D. Minteer. Mammalian cell culture / Simon P. Langdon. Plant tissue culture / Victor M. Loyola-Vargas [and others]. Stem cells and regenerative medicine / Mohan C. Vemuri and Chellu S. Chetty. Cryopreservatioin : conservation of bioresources at ultra low temperatures / John G. Day [and others]. Magnetic resonance imaging / Pottumarthi V. Prasad and Pippa Storey. Electron microscopy / John Kuo. Confocal microscopy / Guy Cox. Laser microdissection / Graeme I. Murray. Biomedical uses of flow cytometry / James L. Weaver and Maryalice Stetler-Stevenson. Immunomicroscopy / Constance Oliver. In situ hybridization / Ian A. Darby and Tim D. Hewitson. High throughput screening / William P. Janzen.
Summary: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR.
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Includes bibliographical references and index.

Nucleic acid methods: The manipulation of nucleic acids : basic tools and techniques / Gayle Corkill and Ralph Rapley. Restriction enzymes : tools in clinical research / Gareth J.S. Jenkins. Principles and medical applications of the polymerase chain reaction / Bimal D.M. Theophilus. Probe design, production, and applications / Marilena Aquino de Muro. Southern blotting as a diagnostic method / Bronwen M. Harvey and Pirkko Soundy. Capillary electrophoresis of DNA : biomedical applications / Beatriz Sanchez-Vega. Denaturing high-performance liquid chromatography (DHPLC) for nucleic acid analysis / Kim Hung Leung and Shea Ping Yip. Denaturing gradient gel electrophoresis (DGGE) / Jeroen H. Roelfsema and Dorien J.M. Peters. Single strand conformation polymorphism (SSCP) analysis / Kim Hung Leung and Shea Ping Yip. Randomly amplified polymorphic DNA (RAPD) : a useful tool for genomic characterization of different organisms / Lúcia Maria da Cunha Galvão and Eliane Lages-Silva. Quantification of mRNA using real time RT-PCR : the SYBR solution / David Sugden and Patricia de Winter. Quantitative analysis of DNA sequences by PCR and solid-phase minisequencing / Anu Suomalainen and Ann-Christine Syvänen. Multiplex amplifiable probe hybridization (MAPH) / Carolina Sismani, Ludmila Kousoulidou, and Philippos C. Patsalis. Gene expression profiling / Arnis Druka, Robbie Waugh, and Pete Hedley. Comparative genomic hybridization in clinical and medical research / Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin. Subtractive hybridization / Craig Winstanley. Fluorescence in situ hybridization / Jane Bayani and Jeremy A. Squire. Quantitative trait locus mapping to identify genes for complex traits in mice / Jonathan D. Smith. cDNA microarrays / Phillip G. Febbo. Mapping techniques / Simon G. Gregory. Single nucleotide polymorphisms / Nameeta Shah. Gene/protein sequence analysis : a compilation of bioinformatic tools / Bernd H.A. Rehm and Frank Reinecke.

Protein and cell methods: Protein electrophoresis / David Sheehan and Siobhan O'Sullivan. Protein blotting / Patricia Gravel. Capillary electrophoresis of proteins / Mark Strege. Autoradiograph and fluorography / Bronwen M. Harvey. Mass spectrometry of proteins and peptides / Kenneth G. Standing. Post-translational modifications of proteins / Christoph Kannich and Birte Fuchs. Protein microarray technology / Charlotte H. Clarke and Eric T. Fung. Protein-protein interactions / Hae Ryoun Park [and others]. Glycoprotein analysis / Terry D. Butters and David C.A. Neville. Solid-phase peptide synthesis / Mare Cudic and Gregg B. Fields. Monoclonal antibodies / Zhong J. Zhang and Maher Albitar. Antibody phage display / Rob Aitken. Protein engineering / Thomas Willemsen [and others]. Directed protein evolution / Sabine C. Stebel [and others]. Enzyme linked immunosorbent assay (ELISA) / John Crowther. Epitope mapping : identification of antibody-binding sites on protein antigens / Glenn E. Morris. Quantum dots / Charles Z. Hotz. Ion-exchange chromatography / David Sheehan. Size-exclusion chromatography / Paul Cutler. Hydrophobic interaction chromatography / Paul A. O'Farrell. Affinity chromatography / Adam Charlton and Michael Zachariou. High performance liquid chromatography (HPLC) of peptides and proteins / Tzong-Hsien Lee and Marie-Isabel Aguilar. Amino acid analysis / Ian Davidson and Paula O'Connor. Surface plasmon resonance / K. Scott Phillips and Quan Jason Cheng. Macromolecular crystallography / Bernhard Rupp and Katherine A. Kantardjieff. Microchip devices for bioanalysis / Anna C. Kinsella and Shelley D. Minteer. Mammalian cell culture / Simon P. Langdon. Plant tissue culture / Victor M. Loyola-Vargas [and others]. Stem cells and regenerative medicine / Mohan C. Vemuri and Chellu S. Chetty. Cryopreservatioin : conservation of bioresources at ultra low temperatures / John G. Day [and others]. Magnetic resonance imaging / Pottumarthi V. Prasad and Pippa Storey. Electron microscopy / John Kuo. Confocal microscopy / Guy Cox. Laser microdissection / Graeme I. Murray. Biomedical uses of flow cytometry / James L. Weaver and Maryalice Stetler-Stevenson. Immunomicroscopy / Constance Oliver. In situ hybridization / Ian A. Darby and Tim D. Hewitson. High throughput screening / William P. Janzen.

Print version record.

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR.

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