Membrane proteomics : methods and protocols / edited by Matthew J. Peirce and Robin Wait.
Contributor(s): Peirce, Matthew J | Wait, RobinMaterial type: TextSeries: Methods in molecular biology (Clifton, N.J.): v. 528.; Springer protocols (Series): Publisher: New York, NY : Humana Press, ©2009Description: 1 online resource (xiii, 342 pages) : illustrationsContent type: text Media type: computer Carrier type: online resourceISBN: 9781603273091; 1603273093; 9781603273107; 1603273107Subject(s): Membrane proteins | Proteomics | Membrane Proteins -- analysis | Proteomics -- methods | Membrane proteins | Proteomics | Protéines membranaires -- analyse | Biochemistry | Animal Biochemistry | Chemistry | Human Anatomy & Physiology | Health & Biological Sciences | Physical Sciences & MathematicsGenre/Form: Electronic books. | Elektronisches Buch -- Formschlagwort. Additional physical formats: Print version:: Membrane proteomics.DDC classification: 572/.696 LOC classification: QP552.M44 | M483 2009Online resources: Click here to access online
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Includes bibliographical references and index.
Online tools for predicting integral membrane proteins / H. Bigelow and B. Rost -- In silico identification of novel G protein coupled receptors / M.N. Davies and D.R. Flower -- Transcriptome-based identification of candidate membrane proteins / E.J. Evans [and others] -- Separation of thylakoid membrane proteins by sucrose gradient ultracentrifugation or blue native-SDS-PAGE two-dimensional electrophoresis / G.M. D'Amici, C.G. Huber and L. Zolla -- Extraction of yeast mitochondrial membrane proteins by solubilization and detergent/polymer aqueous two-phase partitioning / H. Everberg, N. Gustavsson and F. Tjerneld -- 16-BAC/SDS-PAGE analysis of membrane proteins of yeast mitochondria purified by free flow electrophoresis / R.J. Braun [and others] -- Sequential detergent extraction prior to mass spectrometry analysis / F.M. McCarthy, A.M. Cooksey and S.C. Burgess -- Enrichment of brain plasma membranes by affinity two-phase partitioning / J. Schindler and H.G. Nothwang -- Protocol to enrich and analyze plasma membrane proteins / R.J. Wisniewski -- Proteomic analysis of the lymphocyte plasma membrane using cell surface biotinylation and solution-phase isoelectric focusing / M.J. Peirce, A.P. Cope and R. Wait -- Identification of target membrane proteins as detected by phage antibodies / C.A. Geuijen, A.Q. Bakker and J. de Kruif -- Membrane protease degradomics: proteomic identification and quantification of cell surface protease substrates / G.S. Butler [and others] -- Purification of basolateral integral membrane proteins by cationic colloidal silica-based apical membrane subtraction / R.J. Goode and R.J. Simpson -- Moving closer to the lipid raft proteome using quantitative proteomics / L.J. Foster -- Use of sequential chemical extractions to purify nuclear membrane proteins for proteomics identification / N. Korfali [and others] -- Isolation of extracellular membranous vesicles for proteomic analysis / R.A. Mathias [and others] -- Enrichment of human platelet membranes for proteomic analysis / D.W. Greening [and others] -- Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis / T. Rabilloud -- Two-dimensional separation of membrane proteins by 16-BAC-SDS-PAGE / H.G. Nothwang and J. Schindler -- MudPIT analysis: application to human heart tissue / K.G. Kline and C.C. Wu -- Liquid chromatography MALDI MS/MS for membrane proteome analysis / N. Wang, J.B. Young and L. Li -- Cysteinyl-tagging of integral membrane proteins for proteomic analysis using liquid chromatography-tandem mass spectrometry / S.K. Mitra and M.B. Goshe -- Quantitative proteomics analysis of pancreatic zymogen granule membrane proteins / X. Chen and P.C. Andrews.
Print version record.
With the great cellular and therapeutic importance of plasma membrane proteins, unbiased technologies such as proteomics become even more vital as they have the power to define patterns of membrane protein expression characteristic of distinct states of cellular development, differentiation or disease, and thereby identify novel markers of, or targets for intervention in, disease. In Membrane Proteomics: Methods and Protocols, leading experts in the field compile a laboratory bench resource which provides a comprehensive toolkit of proven methods. The volume delves into various modifications to standard two-dimensional gel electrophoresis protocols as well as liquid chromatographic methods, protocols for the enrichment of diverse classes of plasma membrane proteins, in silico approaches, and cutting-edge techniques for quantitative membrane protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-use, Membrane Proteomics: Methods and Protocols is an ideal guide for investigators wishing to apply state of the art membrane proteomic methodologies in their own research programs.