Gene knockout protocols.

Contributor(s): Kühn, Ralf | Wurst, Wolfgang, 1955-
Material type: TextTextSeries: Methods in molecular biology (Clifton, N.J.): v. 530.; Springer protocols (Series): Publisher: New York, NY : Humana Press, ©2009Edition: 2nd ed. / edited by Ralf Khün, Wolfgang WurstDescription: 1 online resource (xvi, 516 pages, 4 unnumbered pages of plates) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9781597454711; 1597454710Subject(s): Gene targeting | Transgenic mice | Gene Targeting -- methods | Mice, Knockout | Mice, Transgenic | Gene targeting | Transgenic mice | Souris transgéniques | Ciblage gène | Mutante | Transgene Tiere | Gen-Targeting | Aufsatzsammlung | Génétique | Maus | Gen | genen | genes | mutagenese | mutagenesis | embryonale stamcellen | embryonic stem cells | inactivering van genen | gene knock-out | gene discovery | Molecular Biology (General) | Moleculaire biologie (algemeen)Genre/Form: Laboratory Manual. | Electronic books. Additional physical formats: Print version:: Gene knockout protocols.DDC classification: 572.8/77 LOC classification: QH442.3Online resources: Click here to access online
Contents:
Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst -- Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu -- Gene-trap vectors and mutagenesis / Silke De-Zolt [and others] -- Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley -- Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts [and others] -- Generation of shRNA transgenic mice / Christiane Hitz [and others] -- Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti -- Gene targeting in mouse embryonic stem cells / Lino Tessarollo [and others] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder [and others] -- Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama -- Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo -- Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka [and others] -- VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara [and others] -- Generation of Cre recombinase-expressing transgenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz -- Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil -- Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts -- Transposon mutagenesis in mice / David A. Largaespada -- Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann -- Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis -- Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman -- Pathologic phenotyping of mutant mice / Roderick T. Bronson -- Systemic first-line phenotyping / Valérie Gailus-Durner [and others].
Abstract: The volume provides a comprehensive collection of step-by-step protocols of use not only for the beginner in the field but also for experienced scientists. This edition particularly emphasizes the range of new mutagenesis techniques developed over the last seven years, but also covers the basic methods relevant to researchers performing classical gene targeting experiments.
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Includes bibliographical references and index.

Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst -- Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu -- Gene-trap vectors and mutagenesis / Silke De-Zolt [and others] -- Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley -- Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts [and others] -- Generation of shRNA transgenic mice / Christiane Hitz [and others] -- Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti -- Gene targeting in mouse embryonic stem cells / Lino Tessarollo [and others] -- Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo -- ES cell line establishment / Heidrun Kern and Branko Zevnik -- Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele -- Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder [and others] -- Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama -- Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo -- Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka [and others] -- VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara [and others] -- Generation of Cre recombinase-expressing transgenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz -- Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil -- Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts -- Transposon mutagenesis in mice / David A. Largaespada -- Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann -- Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis -- Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman -- Pathologic phenotyping of mutant mice / Roderick T. Bronson -- Systemic first-line phenotyping / Valérie Gailus-Durner [and others].

Print version record.

The volume provides a comprehensive collection of step-by-step protocols of use not only for the beginner in the field but also for experienced scientists. This edition particularly emphasizes the range of new mutagenesis techniques developed over the last seven years, but also covers the basic methods relevant to researchers performing classical gene targeting experiments.

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